Isolation of Peripheral Blood Plasma Cells and Co-Culture with Bone Marrow Stromal Cells in Order to Long-Term Culture
Background: Single B cell antibody technology is a novel strategy to make human monoclonal antibody (mAb). Its main part is long-term culture of primary plasma cells (PC) to secrete antibody. PC survival in bone marrow is related to environmental signals, which should supply using a feeder cell mono...
Main Authors: | , , , |
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Format: | Article |
Language: | fas |
Published: |
Isfahan University of Medical Sciences
2014-06-01
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Series: | مجله دانشکده پزشکی اصفهان |
Subjects: | |
Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/3644 |