Determination of the Degree of Unsaturation in Essential Oils
Being widely used, medicinal products based on vegetable oils require strict regulation and evaluation of quality attributes, determination of shelf-life periods, and monitoring of storage conditions. The most common testing method for unsaturated compounds in vegetable oils is the iodine value dete...
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| Format: | Article |
| Language: | Russian |
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NEICON ISP LLC
2023-03-01
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| Series: | Ведомости Научного центра экспертизы средств медицинского применения |
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| Online Access: | https://www.vedomostincesmp.ru/jour/article/view/492 |
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| author | A. M. Agafonov I. P. Remezova V. V. Kosenko N. D. Bunyatyan L. S. Anosovа V. A. Evteev |
| author_facet | A. M. Agafonov I. P. Remezova V. V. Kosenko N. D. Bunyatyan L. S. Anosovа V. A. Evteev |
| author_sort | A. M. Agafonov |
| collection | DOAJ |
| description | Being widely used, medicinal products based on vegetable oils require strict regulation and evaluation of quality attributes, determination of shelf-life periods, and monitoring of storage conditions. The most common testing method for unsaturated compounds in vegetable oils is the iodine value determination, which has a range of limitations. An alternative method for determining the degree of unsaturation is based on epoxidation.The aim of the study was to evaluate the possibility of determining the degree of unsaturation of terpenoids and essential oils using peroxycarboxylic acids.Materials and methods. The authors performed epoxidation of linalool, myrcene, and lemon oil with peroxydecanoic and peroxyoctanoic acids, followed by iodometric titration of the excess acid.Results. The study demonstrated the possibility of measuring the degree of unsaturation of the selected essential oil and terpenoids using peracid epoxidation. The authors developed a procedure for determining the iodine value of essential oils and calculated the iodine values of linalool, myrcene, and lemon oil. Epoxidation proceeded as a second-order reaction. The authors obtained the following reaction rate constants: 3.9 L×mol–1×min–1 for linalool (298 К), 1.76 L×mol–1×min–1 for myrcene converting to monoxide (298 К), 0.044 L×mol–1×min–1 for myrcene epoxide converting to diepoxide (298 К), and 3.9 L×mol–1×min–1 for lemon oil (297 К).Conclusions. The suggested procedure involving peracid titration for rapid and efficient determination of the degree of unsaturation (iodine value) provides a potential basis for developing a quantification method for total unsaturated bioactive compounds in essential oils. |
| first_indexed | 2024-03-07T19:16:53Z |
| format | Article |
| id | doaj.art-316cb81bce8f42a9989df1539ea68de2 |
| institution | Directory Open Access Journal |
| issn | 1991-2919 2619-1172 |
| language | Russian |
| last_indexed | 2024-04-24T07:08:23Z |
| publishDate | 2023-03-01 |
| publisher | NEICON ISP LLC |
| record_format | Article |
| series | Ведомости Научного центра экспертизы средств медицинского применения |
| spelling | doaj.art-316cb81bce8f42a9989df1539ea68de22024-04-21T11:46:35ZrusNEICON ISP LLCВедомости Научного центра экспертизы средств медицинского применения1991-29192619-11722023-03-0113111212010.30895/1991-2919-2023-492374Determination of the Degree of Unsaturation in Essential OilsA. M. Agafonov0I. P. Remezova1V. V. Kosenko2N. D. Bunyatyan3L. S. Anosovа4V. A. Evteev5M. Gorky Donetsk National Medical UniversityPyatigorsk Medical and Pharmaceutical Institute, branch of the Volgograd State Medical UniversityScientific Centre for Expert Evaluation of Medicinal ProductsScientific Centre for Expert Evaluation of Medicinal Products; I.M. Sechenov First Moscow State Medical University (Sechenov University)M. Gorky Donetsk National Medical UniversityScientific Centre for Expert Evaluation of Medicinal ProductsBeing widely used, medicinal products based on vegetable oils require strict regulation and evaluation of quality attributes, determination of shelf-life periods, and monitoring of storage conditions. The most common testing method for unsaturated compounds in vegetable oils is the iodine value determination, which has a range of limitations. An alternative method for determining the degree of unsaturation is based on epoxidation.The aim of the study was to evaluate the possibility of determining the degree of unsaturation of terpenoids and essential oils using peroxycarboxylic acids.Materials and methods. The authors performed epoxidation of linalool, myrcene, and lemon oil with peroxydecanoic and peroxyoctanoic acids, followed by iodometric titration of the excess acid.Results. The study demonstrated the possibility of measuring the degree of unsaturation of the selected essential oil and terpenoids using peracid epoxidation. The authors developed a procedure for determining the iodine value of essential oils and calculated the iodine values of linalool, myrcene, and lemon oil. Epoxidation proceeded as a second-order reaction. The authors obtained the following reaction rate constants: 3.9 L×mol–1×min–1 for linalool (298 К), 1.76 L×mol–1×min–1 for myrcene converting to monoxide (298 К), 0.044 L×mol–1×min–1 for myrcene epoxide converting to diepoxide (298 К), and 3.9 L×mol–1×min–1 for lemon oil (297 К).Conclusions. The suggested procedure involving peracid titration for rapid and efficient determination of the degree of unsaturation (iodine value) provides a potential basis for developing a quantification method for total unsaturated bioactive compounds in essential oils.https://www.vedomostincesmp.ru/jour/article/view/492terpenoidsepoxidationperoxy acidlinaloolmyrcenelemon oilunsaturation testtitrimetryiodine valueessential oils |
| spellingShingle | A. M. Agafonov I. P. Remezova V. V. Kosenko N. D. Bunyatyan L. S. Anosovа V. A. Evteev Determination of the Degree of Unsaturation in Essential Oils Ведомости Научного центра экспертизы средств медицинского применения terpenoids epoxidation peroxy acid linalool myrcene lemon oil unsaturation test titrimetry iodine value essential oils |
| title | Determination of the Degree of Unsaturation in Essential Oils |
| title_full | Determination of the Degree of Unsaturation in Essential Oils |
| title_fullStr | Determination of the Degree of Unsaturation in Essential Oils |
| title_full_unstemmed | Determination of the Degree of Unsaturation in Essential Oils |
| title_short | Determination of the Degree of Unsaturation in Essential Oils |
| title_sort | determination of the degree of unsaturation in essential oils |
| topic | terpenoids epoxidation peroxy acid linalool myrcene lemon oil unsaturation test titrimetry iodine value essential oils |
| url | https://www.vedomostincesmp.ru/jour/article/view/492 |
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