Sampling and detection of airborne influenza virus towards point-of-care applications.
Airborne transmission of the influenza virus contributes significantly to the spread of this infectious pathogen, particularly over large distances when carried by aerosol droplets with long survival times. Efficient sampling of virus-loaded aerosol in combination with a low limit of detection of th...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5369763?pdf=render |
| _version_ | 1811213330694537216 |
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| author | Laila Ladhani Gaspard Pardon Hanne Meeuws Liesbeth van Wesenbeeck Kristiane Schmidt Lieven Stuyver Wouter van der Wijngaart |
| author_facet | Laila Ladhani Gaspard Pardon Hanne Meeuws Liesbeth van Wesenbeeck Kristiane Schmidt Lieven Stuyver Wouter van der Wijngaart |
| author_sort | Laila Ladhani |
| collection | DOAJ |
| description | Airborne transmission of the influenza virus contributes significantly to the spread of this infectious pathogen, particularly over large distances when carried by aerosol droplets with long survival times. Efficient sampling of virus-loaded aerosol in combination with a low limit of detection of the collected virus could enable rapid and early detection of airborne influenza virus at the point-of-care setting. Here, we demonstrate a successful sampling and detection of airborne influenza virus using a system specifically developed for such applications. Our system consists of a custom-made electrostatic precipitation (ESP)-based bioaerosol sampler that is coupled with downstream quantitative polymerase chain reaction (qPCR) analysis. Aerosolized viruses are sampled directly into a miniaturized collector with liquid volume of 150 μL, which constitutes a simple and direct interface with subsequent biological assays. This approach reduces sample dilution by at least one order of magnitude when compared to other liquid-based aerosol bio-samplers. Performance of our ESP-based sampler was evaluated using influenza virus-loaded sub-micron aerosols generated from both cultured and clinical samples. Despite the miniaturized collection volume, we demonstrate a collection efficiency of at least 10% and sensitive detection of a minimum of 3721 RNA copies. Furthermore, we show that an improved extraction protocol can allow viral recovery of down to 303 RNA copies and a maximum sampler collection efficiency of 47%. A device with such a performance would reduce sampling times dramatically, from a few hours with current sampling methods down to a couple of minutes with our ESP-based bioaerosol sampler. |
| first_indexed | 2024-04-12T05:43:58Z |
| format | Article |
| id | doaj.art-316cc61f2f224a51b7d0b9dc41e6b407 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T05:43:58Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-316cc61f2f224a51b7d0b9dc41e6b4072022-12-22T03:45:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01123e017431410.1371/journal.pone.0174314Sampling and detection of airborne influenza virus towards point-of-care applications.Laila LadhaniGaspard PardonHanne MeeuwsLiesbeth van WesenbeeckKristiane SchmidtLieven StuyverWouter van der WijngaartAirborne transmission of the influenza virus contributes significantly to the spread of this infectious pathogen, particularly over large distances when carried by aerosol droplets with long survival times. Efficient sampling of virus-loaded aerosol in combination with a low limit of detection of the collected virus could enable rapid and early detection of airborne influenza virus at the point-of-care setting. Here, we demonstrate a successful sampling and detection of airborne influenza virus using a system specifically developed for such applications. Our system consists of a custom-made electrostatic precipitation (ESP)-based bioaerosol sampler that is coupled with downstream quantitative polymerase chain reaction (qPCR) analysis. Aerosolized viruses are sampled directly into a miniaturized collector with liquid volume of 150 μL, which constitutes a simple and direct interface with subsequent biological assays. This approach reduces sample dilution by at least one order of magnitude when compared to other liquid-based aerosol bio-samplers. Performance of our ESP-based sampler was evaluated using influenza virus-loaded sub-micron aerosols generated from both cultured and clinical samples. Despite the miniaturized collection volume, we demonstrate a collection efficiency of at least 10% and sensitive detection of a minimum of 3721 RNA copies. Furthermore, we show that an improved extraction protocol can allow viral recovery of down to 303 RNA copies and a maximum sampler collection efficiency of 47%. A device with such a performance would reduce sampling times dramatically, from a few hours with current sampling methods down to a couple of minutes with our ESP-based bioaerosol sampler.http://europepmc.org/articles/PMC5369763?pdf=render |
| spellingShingle | Laila Ladhani Gaspard Pardon Hanne Meeuws Liesbeth van Wesenbeeck Kristiane Schmidt Lieven Stuyver Wouter van der Wijngaart Sampling and detection of airborne influenza virus towards point-of-care applications. PLoS ONE |
| title | Sampling and detection of airborne influenza virus towards point-of-care applications. |
| title_full | Sampling and detection of airborne influenza virus towards point-of-care applications. |
| title_fullStr | Sampling and detection of airborne influenza virus towards point-of-care applications. |
| title_full_unstemmed | Sampling and detection of airborne influenza virus towards point-of-care applications. |
| title_short | Sampling and detection of airborne influenza virus towards point-of-care applications. |
| title_sort | sampling and detection of airborne influenza virus towards point of care applications |
| url | http://europepmc.org/articles/PMC5369763?pdf=render |
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