Transcriptome Analysis and Intraspecific Variation in Spanish Fir (<i>Abies pinsapo</i> Boiss.)
Spanish fir (<i>Abies pinsapo</i> Boiss.) is an endemic, endangered tree that has been scarcely investigated at the molecular level. In this work, the transcriptome of Spanish fir was assembled, providing a large catalog of expressed genes (22,769), within which a high proportion were fu...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/9351 |
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| author | Francisco Ortigosa Concepción Ávila Lourdes Rubio Lucía Álvarez-Garrido José A. Carreira Rafael A. Cañas Francisco M. Cánovas |
| author_facet | Francisco Ortigosa Concepción Ávila Lourdes Rubio Lucía Álvarez-Garrido José A. Carreira Rafael A. Cañas Francisco M. Cánovas |
| author_sort | Francisco Ortigosa |
| collection | DOAJ |
| description | Spanish fir (<i>Abies pinsapo</i> Boiss.) is an endemic, endangered tree that has been scarcely investigated at the molecular level. In this work, the transcriptome of Spanish fir was assembled, providing a large catalog of expressed genes (22,769), within which a high proportion were full-length transcripts (12,545). This resource is valuable for functional genomics studies and genome annotation in this relict conifer species. Two intraspecific variations of <i>A. pinsapo</i> can be found within its largest population at the <i>Sierra de las Nieves</i> National Park: one with standard green needles and another with bluish-green needles. To elucidate the causes of both phenotypes, we studied different physiological and molecular markers and transcriptome profiles in the needles. “Green” trees showed higher electron transport efficiency and enhanced levels of chlorophyll, protein, and total nitrogen in the needles. In contrast, needles from “bluish” trees exhibited higher contents of carotenoids and cellulose. These results agreed with the differential transcriptomic profiles, suggesting an imbalance in the nitrogen status of “bluish” trees. Additionally, gene expression analyses suggested that these differences could be associated with different epigenomic profiles. Taken together, the reported data provide new transcriptome resources and a better understanding of the natural variation in this tree species, which can help improve guidelines for its conservation and the implementation of adaptive management strategies under climatic change. |
| first_indexed | 2024-03-09T13:15:17Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T13:15:17Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-316e5c063f8e4a1aae1fc5069ab6d8b92023-11-30T21:36:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316935110.3390/ijms23169351Transcriptome Analysis and Intraspecific Variation in Spanish Fir (<i>Abies pinsapo</i> Boiss.)Francisco Ortigosa0Concepción Ávila1Lourdes Rubio2Lucía Álvarez-Garrido3José A. Carreira4Rafael A. Cañas5Francisco M. Cánovas6Grupo de Biología Molecular y Biotecnología, Departamento de Biología Molecular y Bioquímica, Universidad de Málaga, Campus Universitario de Teatinos, 29071 Málaga, SpainGrupo de Biología Molecular y Biotecnología, Departamento de Biología Molecular y Bioquímica, Universidad de Málaga, Campus Universitario de Teatinos, 29071 Málaga, SpainDepartamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Campus Universitario de Teatinos, 29071 Málaga, SpainDepartment of Ecology, Universidad de Jaén, Campus Las Lagunillas s/n., 23009 Jaén, SpainDepartment of Ecology, Universidad de Jaén, Campus Las Lagunillas s/n., 23009 Jaén, SpainIntegrative Molecular Biology Lab, Universidad de Málaga, Campus Universitario de Teatinos, 29071 Málaga, SpainGrupo de Biología Molecular y Biotecnología, Departamento de Biología Molecular y Bioquímica, Universidad de Málaga, Campus Universitario de Teatinos, 29071 Málaga, SpainSpanish fir (<i>Abies pinsapo</i> Boiss.) is an endemic, endangered tree that has been scarcely investigated at the molecular level. In this work, the transcriptome of Spanish fir was assembled, providing a large catalog of expressed genes (22,769), within which a high proportion were full-length transcripts (12,545). This resource is valuable for functional genomics studies and genome annotation in this relict conifer species. Two intraspecific variations of <i>A. pinsapo</i> can be found within its largest population at the <i>Sierra de las Nieves</i> National Park: one with standard green needles and another with bluish-green needles. To elucidate the causes of both phenotypes, we studied different physiological and molecular markers and transcriptome profiles in the needles. “Green” trees showed higher electron transport efficiency and enhanced levels of chlorophyll, protein, and total nitrogen in the needles. In contrast, needles from “bluish” trees exhibited higher contents of carotenoids and cellulose. These results agreed with the differential transcriptomic profiles, suggesting an imbalance in the nitrogen status of “bluish” trees. Additionally, gene expression analyses suggested that these differences could be associated with different epigenomic profiles. Taken together, the reported data provide new transcriptome resources and a better understanding of the natural variation in this tree species, which can help improve guidelines for its conservation and the implementation of adaptive management strategies under climatic change.https://www.mdpi.com/1422-0067/23/16/9351conifer<i>Abies pinsapo</i>transcriptomeintraspecific variationchlorophyll fluorescencenitrogen status |
| spellingShingle | Francisco Ortigosa Concepción Ávila Lourdes Rubio Lucía Álvarez-Garrido José A. Carreira Rafael A. Cañas Francisco M. Cánovas Transcriptome Analysis and Intraspecific Variation in Spanish Fir (<i>Abies pinsapo</i> Boiss.) International Journal of Molecular Sciences conifer <i>Abies pinsapo</i> transcriptome intraspecific variation chlorophyll fluorescence nitrogen status |
| title | Transcriptome Analysis and Intraspecific Variation in Spanish Fir (<i>Abies pinsapo</i> Boiss.) |
| title_full | Transcriptome Analysis and Intraspecific Variation in Spanish Fir (<i>Abies pinsapo</i> Boiss.) |
| title_fullStr | Transcriptome Analysis and Intraspecific Variation in Spanish Fir (<i>Abies pinsapo</i> Boiss.) |
| title_full_unstemmed | Transcriptome Analysis and Intraspecific Variation in Spanish Fir (<i>Abies pinsapo</i> Boiss.) |
| title_short | Transcriptome Analysis and Intraspecific Variation in Spanish Fir (<i>Abies pinsapo</i> Boiss.) |
| title_sort | transcriptome analysis and intraspecific variation in spanish fir i abies pinsapo i boiss |
| topic | conifer <i>Abies pinsapo</i> transcriptome intraspecific variation chlorophyll fluorescence nitrogen status |
| url | https://www.mdpi.com/1422-0067/23/16/9351 |
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