Bioprospects of Endophytic Bacteria in Plant Growth Promotion and Ag-Nanoparticle Biosynthesis
In this study, five endophytic bacterial strains, namely <i>Rhizobium pusense</i> (MS-1), <i>Bacillus cereus</i> MS-2, <i>Bacillus flexus</i> (MS-3), <i>Methylophilus flavus</i> (MS-4), and <i>Pseudomonas aeruginosa</i> (MS-5), were used to...
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MDPI AG
2022-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/14/1787 |
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| author | Monika Singh Kamal A. Qureshi Mariusz Jaremko Minakshi Rajput Sandeep Kumar Singh Kaushalendra Kapil D. Pandey Luiz Fernando Romanholo Ferreira Ajay Kumar |
| author_facet | Monika Singh Kamal A. Qureshi Mariusz Jaremko Minakshi Rajput Sandeep Kumar Singh Kaushalendra Kapil D. Pandey Luiz Fernando Romanholo Ferreira Ajay Kumar |
| author_sort | Monika Singh |
| collection | DOAJ |
| description | In this study, five endophytic bacterial strains, namely <i>Rhizobium pusense</i> (MS-1), <i>Bacillus cereus</i> MS-2, <i>Bacillus flexus</i> (MS-3), <i>Methylophilus flavus</i> (MS-4), and <i>Pseudomonas aeruginosa</i> (MS-5), were used to investigate their potential role in the enhancement of growth yields of two types of tomato varieties, viz. hybrid and local, and in the biosynthesis of silver nanoparticles (AgNPs). The inoculation of bacterial strains enhanced the root and shoot length, biomass, and leaf chlorophyll contents. The fruit weight of the tomato (kg/plant) was also higher in the bacteria inoculated plants of both hybrid and local varieties than in the control (untreated). A significant increase was recorded in the fruit yield (g/plant) in all the treatments, whereas <i>Methylophilus flavus</i> (MS-4) inoculated plants yielded nearly 2.5 times more fruit weight compared to the control in the hybrid variety and two times higher in the local variety. The response to <i>M. flavus</i> as a microbial inoculant was greater than to the other strains. Biosynthesis of Ag nanoparticles was also carried out using all five endophytic bacterial strains. The weakest producers of AgNPs were <i>Rhizobium pusense</i> (MS-1) and <i>Methylophilus flavus</i> (MS-4), while <i>Bacillus cereus</i> MS-2, <i>Bacillus flexus</i> (MS-3), and <i>Pseudomonas aeruginosa</i> (MS-5) were strong producers of AgNPs. Nanoparticles were further characterized using high-resolution scanning electron microscopy (HR-SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR), UV-Vis spectrophotometry, and X-ray diffraction (XRD) analysis, and revealed cuboidal shaped AgNPs in the <i>Bacillus cereus</i> MS-2 strain. In addition, the biosynthesized AgNPs showed antibacterial activity against various pathogenic and endophytic bacterial strains. |
| first_indexed | 2024-03-09T10:13:13Z |
| format | Article |
| id | doaj.art-316e6482f0284c99bc88da887754c355 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T10:13:13Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-316e6482f0284c99bc88da887754c3552023-12-01T22:35:31ZengMDPI AGPlants2223-77472022-07-011114178710.3390/plants11141787Bioprospects of Endophytic Bacteria in Plant Growth Promotion and Ag-Nanoparticle BiosynthesisMonika Singh0Kamal A. Qureshi1Mariusz Jaremko2Minakshi Rajput3Sandeep Kumar Singh4Kaushalendra5Kapil D. Pandey6Luiz Fernando Romanholo Ferreira7Ajay Kumar8Department of Biotechnology, School of Applied and Life Sciences, Uttaranchal University, Dehradun 248007, IndiaDepartment of Pharmaceutics, Unaizah College of Pharmacy, Qassim University, Unaizah 51911, Saudi ArabiaSmart-Health Initiative (SHI) and Red Sea Research Center (RSRC), Division of Biological and Environment Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi ArabiaDepartment of Biotechnology, School of Applied and Life Sciences, Uttaranchal University, Dehradun 248007, IndiaDivision of Microbiology, Indian Agricultural Research Institute, Pusa, New Delhi 110012, IndiaDepartment of Zoology, Pachhunga University College Campus, Mizoram University (a Central University), Aizawl 796001, IndiaCentre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, IndiaInstitute for Technology and Research (ITP), Tiradentes University-UNIT, Aracaju 49032-490, SE, BrazilCentre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, IndiaIn this study, five endophytic bacterial strains, namely <i>Rhizobium pusense</i> (MS-1), <i>Bacillus cereus</i> MS-2, <i>Bacillus flexus</i> (MS-3), <i>Methylophilus flavus</i> (MS-4), and <i>Pseudomonas aeruginosa</i> (MS-5), were used to investigate their potential role in the enhancement of growth yields of two types of tomato varieties, viz. hybrid and local, and in the biosynthesis of silver nanoparticles (AgNPs). The inoculation of bacterial strains enhanced the root and shoot length, biomass, and leaf chlorophyll contents. The fruit weight of the tomato (kg/plant) was also higher in the bacteria inoculated plants of both hybrid and local varieties than in the control (untreated). A significant increase was recorded in the fruit yield (g/plant) in all the treatments, whereas <i>Methylophilus flavus</i> (MS-4) inoculated plants yielded nearly 2.5 times more fruit weight compared to the control in the hybrid variety and two times higher in the local variety. The response to <i>M. flavus</i> as a microbial inoculant was greater than to the other strains. Biosynthesis of Ag nanoparticles was also carried out using all five endophytic bacterial strains. The weakest producers of AgNPs were <i>Rhizobium pusense</i> (MS-1) and <i>Methylophilus flavus</i> (MS-4), while <i>Bacillus cereus</i> MS-2, <i>Bacillus flexus</i> (MS-3), and <i>Pseudomonas aeruginosa</i> (MS-5) were strong producers of AgNPs. Nanoparticles were further characterized using high-resolution scanning electron microscopy (HR-SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR), UV-Vis spectrophotometry, and X-ray diffraction (XRD) analysis, and revealed cuboidal shaped AgNPs in the <i>Bacillus cereus</i> MS-2 strain. In addition, the biosynthesized AgNPs showed antibacterial activity against various pathogenic and endophytic bacterial strains.https://www.mdpi.com/2223-7747/11/14/1787endophytic bacteriaplant growth promotiontomatoAg-nanoparticlesHR-SEMTEM |
| spellingShingle | Monika Singh Kamal A. Qureshi Mariusz Jaremko Minakshi Rajput Sandeep Kumar Singh Kaushalendra Kapil D. Pandey Luiz Fernando Romanholo Ferreira Ajay Kumar Bioprospects of Endophytic Bacteria in Plant Growth Promotion and Ag-Nanoparticle Biosynthesis Plants endophytic bacteria plant growth promotion tomato Ag-nanoparticles HR-SEM TEM |
| title | Bioprospects of Endophytic Bacteria in Plant Growth Promotion and Ag-Nanoparticle Biosynthesis |
| title_full | Bioprospects of Endophytic Bacteria in Plant Growth Promotion and Ag-Nanoparticle Biosynthesis |
| title_fullStr | Bioprospects of Endophytic Bacteria in Plant Growth Promotion and Ag-Nanoparticle Biosynthesis |
| title_full_unstemmed | Bioprospects of Endophytic Bacteria in Plant Growth Promotion and Ag-Nanoparticle Biosynthesis |
| title_short | Bioprospects of Endophytic Bacteria in Plant Growth Promotion and Ag-Nanoparticle Biosynthesis |
| title_sort | bioprospects of endophytic bacteria in plant growth promotion and ag nanoparticle biosynthesis |
| topic | endophytic bacteria plant growth promotion tomato Ag-nanoparticles HR-SEM TEM |
| url | https://www.mdpi.com/2223-7747/11/14/1787 |
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