Utilization of immunochemical methods to detect Phytophthora fraga-riae - a quarantine pathogen of strawberry
Polyclonal antibodies anti-PfP IgG and anti-PfM IgG and monoclonal antibody MAb29 were prepared to detect a quarantine pathogen of strawberry, Phytophthora fragariae. Laboratory rabbits and mice were immunized using purified and unpurified protein extracts from the mycelial mass of the pathogen. All...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2001-06-01
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| Series: | Plant Protection Science |
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| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-200102-0002_utilization-of-immunochemical-methods-to-detect-phytophthora-fraga-riae-a-quarantine-pathogen-of-strawberry.php |
| _version_ | 1797898617037520896 |
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| author | Blanka Pekárová Jiřina Krátká Jakub Slováček |
| author_facet | Blanka Pekárová Jiřina Krátká Jakub Slováček |
| author_sort | Blanka Pekárová |
| collection | DOAJ |
| description | Polyclonal antibodies anti-PfP IgG and anti-PfM IgG and monoclonal antibody MAb29 were prepared to detect a quarantine pathogen of strawberry, Phytophthora fragariae. Laboratory rabbits and mice were immunized using purified and unpurified protein extracts from the mycelial mass of the pathogen. All prepared antibodies were genus-specific, therefore Phytophthora cactorum was also detected. Except for Pythium ultimum, the antibodies did not cross-react with other pathogenic fungi, such as Botrytis cinerea, Colletotrichum acutatum, Fusarium sp., Verticillium albo-atrum. PTA-ELISA was used to test the antibodies. P. fragariae was detected in artificially infected strawberries (cultivars Elsanta, Kama and Vanda) by means of PTA-ELISA, immunoprinting and dot blot. Detection of the pathogen was optimal in undamaged roots or roots with necrotic tips only. At a later stage of infection, when whole roots were necrotic, the crown was more suitable for successful detection. To detect P. fragariae at the early stages of infection it is recommended to use at least two of the three mentioned immunotechniques. |
| first_indexed | 2024-04-10T08:17:05Z |
| format | Article |
| id | doaj.art-3176e663096e4e73a47649c74bb79769 |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:17:05Z |
| publishDate | 2001-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-3176e663096e4e73a47649c74bb797692023-02-23T03:43:23ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412001-06-01372576510.17221/8366-PPSpps-200102-0002Utilization of immunochemical methods to detect Phytophthora fraga-riae - a quarantine pathogen of strawberryBlanka PekárováJiřina KrátkáJakub SlováčekPolyclonal antibodies anti-PfP IgG and anti-PfM IgG and monoclonal antibody MAb29 were prepared to detect a quarantine pathogen of strawberry, Phytophthora fragariae. Laboratory rabbits and mice were immunized using purified and unpurified protein extracts from the mycelial mass of the pathogen. All prepared antibodies were genus-specific, therefore Phytophthora cactorum was also detected. Except for Pythium ultimum, the antibodies did not cross-react with other pathogenic fungi, such as Botrytis cinerea, Colletotrichum acutatum, Fusarium sp., Verticillium albo-atrum. PTA-ELISA was used to test the antibodies. P. fragariae was detected in artificially infected strawberries (cultivars Elsanta, Kama and Vanda) by means of PTA-ELISA, immunoprinting and dot blot. Detection of the pathogen was optimal in undamaged roots or roots with necrotic tips only. At a later stage of infection, when whole roots were necrotic, the crown was more suitable for successful detection. To detect P. fragariae at the early stages of infection it is recommended to use at least two of the three mentioned immunotechniques.https://pps.agriculturejournals.cz/artkey/pps-200102-0002_utilization-of-immunochemical-methods-to-detect-phytophthora-fraga-riae-a-quarantine-pathogen-of-strawberry.phpphytophthora fragariaepolyclonal and monoclonal antibodiespta-elisaimmunoprintingdot blotstrawberry |
| spellingShingle | Blanka Pekárová Jiřina Krátká Jakub Slováček Utilization of immunochemical methods to detect Phytophthora fraga-riae - a quarantine pathogen of strawberry Plant Protection Science phytophthora fragariae polyclonal and monoclonal antibodies pta-elisa immunoprinting dot blot strawberry |
| title | Utilization of immunochemical methods to detect Phytophthora fraga-riae - a quarantine pathogen of strawberry |
| title_full | Utilization of immunochemical methods to detect Phytophthora fraga-riae - a quarantine pathogen of strawberry |
| title_fullStr | Utilization of immunochemical methods to detect Phytophthora fraga-riae - a quarantine pathogen of strawberry |
| title_full_unstemmed | Utilization of immunochemical methods to detect Phytophthora fraga-riae - a quarantine pathogen of strawberry |
| title_short | Utilization of immunochemical methods to detect Phytophthora fraga-riae - a quarantine pathogen of strawberry |
| title_sort | utilization of immunochemical methods to detect phytophthora fraga riae a quarantine pathogen of strawberry |
| topic | phytophthora fragariae polyclonal and monoclonal antibodies pta-elisa immunoprinting dot blot strawberry |
| url | https://pps.agriculturejournals.cz/artkey/pps-200102-0002_utilization-of-immunochemical-methods-to-detect-phytophthora-fraga-riae-a-quarantine-pathogen-of-strawberry.php |
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