Soybean Root Transcriptomics: Insights into Sucrose Signaling at the Crossroads of Nutrient Deficiency and Biotic Stress Responses
Soybean (<i>Glycine max</i>) is an important agricultural crop, but nutrient deficiencies frequently limit soybean production. While research has advanced our understanding of plant responses to long-term nutrient deficiencies, less is known about the signaling pathways and immediate res...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/11/2117 |
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| author | Leela Chandra Manozna Nidumolu Kristina Mae Lorilla Indrani Chakravarty Claudia Uhde-Stone |
| author_facet | Leela Chandra Manozna Nidumolu Kristina Mae Lorilla Indrani Chakravarty Claudia Uhde-Stone |
| author_sort | Leela Chandra Manozna Nidumolu |
| collection | DOAJ |
| description | Soybean (<i>Glycine max</i>) is an important agricultural crop, but nutrient deficiencies frequently limit soybean production. While research has advanced our understanding of plant responses to long-term nutrient deficiencies, less is known about the signaling pathways and immediate responses to certain nutrient deficiencies, such as P<sub>i</sub> and Fe deficiencies. Recent studies have shown that sucrose acts as a long-distance signal that is sent in increased concentrations from the shoot to the root in response to various nutrient deficiencies. Here, we mimicked nutrient deficiency-induced sucrose signaling by adding sucrose directly to the roots. To unravel transcriptomic responses to sucrose acting as a signal, we performed Illumina RNA-sequencing of soybean roots treated with sucrose for 20 min and 40 min, compared to non-sucrose-treated controls. We obtained a total of 260 million paired-end reads, mapping to 61,675 soybean genes, some of which are novel (not yet annotated) transcripts. Of these, 358 genes were upregulated after 20 min, and 2416 were upregulated after 40 min of sucrose exposure. GO (gene ontology) analysis revealed a high proportion of sucrose-induced genes involved in signal transduction, particularly hormone, ROS (reactive oxygen species), and calcium signaling, in addition to regulation of transcription. In addition, GO enrichment analysis indicates that sucrose triggers crosstalk between biotic and abiotic stress responses. |
| first_indexed | 2024-03-11T02:59:23Z |
| format | Article |
| id | doaj.art-3177ff9955f0423da2d08e36bdd5a914 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T02:59:23Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-3177ff9955f0423da2d08e36bdd5a9142023-11-18T08:23:36ZengMDPI AGPlants2223-77472023-05-011211211710.3390/plants12112117Soybean Root Transcriptomics: Insights into Sucrose Signaling at the Crossroads of Nutrient Deficiency and Biotic Stress ResponsesLeela Chandra Manozna Nidumolu0Kristina Mae Lorilla1Indrani Chakravarty2Claudia Uhde-Stone3Department of Biological Sciences, California State University, East Bay, Hayward, CA 94542, USADepartment of Biological Sciences, California State University, East Bay, Hayward, CA 94542, USADepartment of Biological Sciences, California State University, East Bay, Hayward, CA 94542, USADepartment of Biological Sciences, California State University, East Bay, Hayward, CA 94542, USASoybean (<i>Glycine max</i>) is an important agricultural crop, but nutrient deficiencies frequently limit soybean production. While research has advanced our understanding of plant responses to long-term nutrient deficiencies, less is known about the signaling pathways and immediate responses to certain nutrient deficiencies, such as P<sub>i</sub> and Fe deficiencies. Recent studies have shown that sucrose acts as a long-distance signal that is sent in increased concentrations from the shoot to the root in response to various nutrient deficiencies. Here, we mimicked nutrient deficiency-induced sucrose signaling by adding sucrose directly to the roots. To unravel transcriptomic responses to sucrose acting as a signal, we performed Illumina RNA-sequencing of soybean roots treated with sucrose for 20 min and 40 min, compared to non-sucrose-treated controls. We obtained a total of 260 million paired-end reads, mapping to 61,675 soybean genes, some of which are novel (not yet annotated) transcripts. Of these, 358 genes were upregulated after 20 min, and 2416 were upregulated after 40 min of sucrose exposure. GO (gene ontology) analysis revealed a high proportion of sucrose-induced genes involved in signal transduction, particularly hormone, ROS (reactive oxygen species), and calcium signaling, in addition to regulation of transcription. In addition, GO enrichment analysis indicates that sucrose triggers crosstalk between biotic and abiotic stress responses.https://www.mdpi.com/2223-7747/12/11/2117crosstalknutrient deficiencysucrose signalingtranscriptome |
| spellingShingle | Leela Chandra Manozna Nidumolu Kristina Mae Lorilla Indrani Chakravarty Claudia Uhde-Stone Soybean Root Transcriptomics: Insights into Sucrose Signaling at the Crossroads of Nutrient Deficiency and Biotic Stress Responses Plants crosstalk nutrient deficiency sucrose signaling transcriptome |
| title | Soybean Root Transcriptomics: Insights into Sucrose Signaling at the Crossroads of Nutrient Deficiency and Biotic Stress Responses |
| title_full | Soybean Root Transcriptomics: Insights into Sucrose Signaling at the Crossroads of Nutrient Deficiency and Biotic Stress Responses |
| title_fullStr | Soybean Root Transcriptomics: Insights into Sucrose Signaling at the Crossroads of Nutrient Deficiency and Biotic Stress Responses |
| title_full_unstemmed | Soybean Root Transcriptomics: Insights into Sucrose Signaling at the Crossroads of Nutrient Deficiency and Biotic Stress Responses |
| title_short | Soybean Root Transcriptomics: Insights into Sucrose Signaling at the Crossroads of Nutrient Deficiency and Biotic Stress Responses |
| title_sort | soybean root transcriptomics insights into sucrose signaling at the crossroads of nutrient deficiency and biotic stress responses |
| topic | crosstalk nutrient deficiency sucrose signaling transcriptome |
| url | https://www.mdpi.com/2223-7747/12/11/2117 |
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