Protective Effect of <em>Lactiplantibacillus plantarum</em> subsp. <em>plantarum</em> SC-5 on Dextran Sulfate Sodium—Induced Colitis in Mice
Inflammatory bowel disease (IBD) is a specific immune-associated intestinal disease. At present, the conventional treatment for patients is not ideal. Probiotics are widely used in the treatment of IBD patients due to their ability to restore the function of the intestinal mucosal barrier effectivel...
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MDPI AG
2023-02-01
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| श्रृंखला: | Foods |
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| ऑनलाइन पहुंच: | https://www.mdpi.com/2304-8158/12/4/897 |
| _version_ | 1827757366847733760 |
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| author | Ruoran Shi Fazheng Yu Xueyu Hu Yan Liu Yuanyuan Jin Honglin Ren Shiying Lu Jian Guo Jiang Chang Yansong Li Zengshan Liu Xiaoxu Wang Pan Hu |
| author_facet | Ruoran Shi Fazheng Yu Xueyu Hu Yan Liu Yuanyuan Jin Honglin Ren Shiying Lu Jian Guo Jiang Chang Yansong Li Zengshan Liu Xiaoxu Wang Pan Hu |
| author_sort | Ruoran Shi |
| collection | DOAJ |
| description | Inflammatory bowel disease (IBD) is a specific immune-associated intestinal disease. At present, the conventional treatment for patients is not ideal. Probiotics are widely used in the treatment of IBD patients due to their ability to restore the function of the intestinal mucosal barrier effectively and safely. <i>Lactiplantibacillus plantarum</i> subsp. <i>plantarum</i> is a kind of probiotic that exists in the intestines of hosts and is considered to have good probiotic properties. In this study, we evaluated the therapeutic effect of <i>Lactiplantibacillus plantarum</i> subsp. <i>plantarum</i> SC-5 (SC-5) on dextran sulfate sodium (DSS)-induced colitis in C57BL/6J mice. We estimated the effect of SC-5 on the clinical symptoms of mice through a body weight change, colon length, and DAI score. The inhibitory effects of SC-5 on the levels of cytokine IL-1β, IL-6, and TNF-α were determined by ELISA. The protein expression levels of NF-κB, MAPK signaling pathway, and the tight junction proteins occludin, claudin-3, and ZO-1 were verified using Western Blot and immunofluorescence. 16S rRNA was used to verify the modulatory effect of SC-5 on the structure of intestinal microbiota in DSS-induced colitis mice. The results showed that SC-5 could alleviate the clinical symptoms of DSS-induced colitis mice, and significantly reduce the expression of pro-inflammatory cytokines in the colon tissue. It also attenuated the inflammatory response by inhibiting the protein expression of NF-κB and MAPK signaling pathways. SC-5 improved the integrity of the intestinal mucosal barrier by strengthening tight junction proteins. In addition, 16S rRNA sequencing demonstrated that SC-5 was effective in restoring intestinal flora balance, as well as in increasing the relative abundance and diversity of beneficial microbiota. These results indicated that SC-5 has the potential to be developed as a new probiotic candidate that prevents or alleviates IBD. |
| first_indexed | 2024-03-11T08:49:39Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T08:49:39Z |
| publishDate | 2023-02-01 |
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| series | Foods |
| spelling | doaj.art-3178f01a03a64b51a2d30004233360982023-11-16T20:32:04ZengMDPI AGFoods2304-81582023-02-0112489710.3390/foods12040897Protective Effect of <em>Lactiplantibacillus plantarum</em> subsp. <em>plantarum</em> SC-5 on Dextran Sulfate Sodium—Induced Colitis in MiceRuoran Shi0Fazheng Yu1Xueyu Hu2Yan Liu3Yuanyuan Jin4Honglin Ren5Shiying Lu6Jian Guo7Jiang Chang8Yansong Li9Zengshan Liu10Xiaoxu Wang11Pan Hu12State Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaInstitute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, ChinaState Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaInflammatory bowel disease (IBD) is a specific immune-associated intestinal disease. At present, the conventional treatment for patients is not ideal. Probiotics are widely used in the treatment of IBD patients due to their ability to restore the function of the intestinal mucosal barrier effectively and safely. <i>Lactiplantibacillus plantarum</i> subsp. <i>plantarum</i> is a kind of probiotic that exists in the intestines of hosts and is considered to have good probiotic properties. In this study, we evaluated the therapeutic effect of <i>Lactiplantibacillus plantarum</i> subsp. <i>plantarum</i> SC-5 (SC-5) on dextran sulfate sodium (DSS)-induced colitis in C57BL/6J mice. We estimated the effect of SC-5 on the clinical symptoms of mice through a body weight change, colon length, and DAI score. The inhibitory effects of SC-5 on the levels of cytokine IL-1β, IL-6, and TNF-α were determined by ELISA. The protein expression levels of NF-κB, MAPK signaling pathway, and the tight junction proteins occludin, claudin-3, and ZO-1 were verified using Western Blot and immunofluorescence. 16S rRNA was used to verify the modulatory effect of SC-5 on the structure of intestinal microbiota in DSS-induced colitis mice. The results showed that SC-5 could alleviate the clinical symptoms of DSS-induced colitis mice, and significantly reduce the expression of pro-inflammatory cytokines in the colon tissue. It also attenuated the inflammatory response by inhibiting the protein expression of NF-κB and MAPK signaling pathways. SC-5 improved the integrity of the intestinal mucosal barrier by strengthening tight junction proteins. In addition, 16S rRNA sequencing demonstrated that SC-5 was effective in restoring intestinal flora balance, as well as in increasing the relative abundance and diversity of beneficial microbiota. These results indicated that SC-5 has the potential to be developed as a new probiotic candidate that prevents or alleviates IBD.https://www.mdpi.com/2304-8158/12/4/897inflammatory bowel diseasedextran sulfate sodium<i>Lactiplantibacillus plantarum</i> subsp. <i>plantarum</i> SC-5NF-κBMAPKtight junction protein |
| spellingShingle | Ruoran Shi Fazheng Yu Xueyu Hu Yan Liu Yuanyuan Jin Honglin Ren Shiying Lu Jian Guo Jiang Chang Yansong Li Zengshan Liu Xiaoxu Wang Pan Hu Protective Effect of <em>Lactiplantibacillus plantarum</em> subsp. <em>plantarum</em> SC-5 on Dextran Sulfate Sodium—Induced Colitis in Mice Foods inflammatory bowel disease dextran sulfate sodium <i>Lactiplantibacillus plantarum</i> subsp. <i>plantarum</i> SC-5 NF-κB MAPK tight junction protein |
| title | Protective Effect of <em>Lactiplantibacillus plantarum</em> subsp. <em>plantarum</em> SC-5 on Dextran Sulfate Sodium—Induced Colitis in Mice |
| title_full | Protective Effect of <em>Lactiplantibacillus plantarum</em> subsp. <em>plantarum</em> SC-5 on Dextran Sulfate Sodium—Induced Colitis in Mice |
| title_fullStr | Protective Effect of <em>Lactiplantibacillus plantarum</em> subsp. <em>plantarum</em> SC-5 on Dextran Sulfate Sodium—Induced Colitis in Mice |
| title_full_unstemmed | Protective Effect of <em>Lactiplantibacillus plantarum</em> subsp. <em>plantarum</em> SC-5 on Dextran Sulfate Sodium—Induced Colitis in Mice |
| title_short | Protective Effect of <em>Lactiplantibacillus plantarum</em> subsp. <em>plantarum</em> SC-5 on Dextran Sulfate Sodium—Induced Colitis in Mice |
| title_sort | protective effect of em lactiplantibacillus plantarum em subsp em plantarum em sc 5 on dextran sulfate sodium induced colitis in mice |
| topic | inflammatory bowel disease dextran sulfate sodium <i>Lactiplantibacillus plantarum</i> subsp. <i>plantarum</i> SC-5 NF-κB MAPK tight junction protein |
| url | https://www.mdpi.com/2304-8158/12/4/897 |
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