Longitudinal Investigation of the Native Grass Hay from Storage to Market Reveals Mycotoxin-Associated Fungi
This study aimed to characterize the fungal diversity and mycotoxin concentrations of native grass hay in various storage periods. In the present study, the native grass hay samples were collected when stored for 0 d (D0 group), 30 d (D30 group), and 150 d (D150 group), respectively. Here, mycotoxin...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/6/1154 |
| _version_ | 1797484250418642944 |
|---|---|
| author | Shuai Du Sihan You Xiaowei Jiang Yuyu Li Yushan Jia |
| author_facet | Shuai Du Sihan You Xiaowei Jiang Yuyu Li Yushan Jia |
| author_sort | Shuai Du |
| collection | DOAJ |
| description | This study aimed to characterize the fungal diversity and mycotoxin concentrations of native grass hay in various storage periods. In the present study, the native grass hay samples were collected when stored for 0 d (D0 group), 30 d (D30 group), and 150 d (D150 group), respectively. Here, mycotoxin analyses combined with ITS gene sequence were performed to reveal the changes in response to the storage period. There were notable differences in deoxynivalenol and aflatoxin B<sub>1</sub> concentrations among the three groups. Compared to the D150 group, the diversity of the fungal community was higher in the D0 and D30 groups, which indicating the diversity was significantly influenced by the storage period. No significant (<i>p</i> > 0.05) difference was observed among the three groups on the dominant phyla. Interestingly, a significant (<i>p</i> < 0.05) difference was also observed in <i>Chactomella</i> and <i>Aspergillus</i> among the three groups, the abundance of the <i>Chactomella</i> was significantly (<i>p</i> < 0.05) decreased and the abundance of <i>Aspergillus</i> was statistically (<i>p</i> < 0.05) increased in the D150 group. Correlation analysis of the association of fungi with mycotoxin could provide a comprehensive understanding of the structure and function of the fungal community. These results indicated that the good practices of storage are essential for the prevention of mycotoxin. The information contained in the present study is vital for the further development of strategies for hay storage with high quality in the harsh Mongolian Plateau ecosystem. |
| first_indexed | 2024-03-09T22:59:50Z |
| format | Article |
| id | doaj.art-317995fd03f44b2da0a68b9145a5251f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T22:59:50Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-317995fd03f44b2da0a68b9145a5251f2023-11-23T18:03:52ZengMDPI AGMicroorganisms2076-26072022-06-01106115410.3390/microorganisms10061154Longitudinal Investigation of the Native Grass Hay from Storage to Market Reveals Mycotoxin-Associated FungiShuai Du0Sihan You1Xiaowei Jiang2Yuyu Li3Yushan Jia4National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, Key Laboratory of Molecular Nutrition, Ministry of Education, Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Nutrition and Feed Science of Zhejiang Province, Institute of Feed Science, Zhejiang University, Hangzhou 310058, ChinaKey Laboratory of Forage Cultivation, Processing and High Efficient Utilization, Ministry of Agriculture, Key Laboratory of Grassland Resources, Ministry of Education, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010019, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010020, ChinaKey Laboratory of Forage Cultivation, Processing and High Efficient Utilization, Ministry of Agriculture, Key Laboratory of Grassland Resources, Ministry of Education, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010019, ChinaKey Laboratory of Forage Cultivation, Processing and High Efficient Utilization, Ministry of Agriculture, Key Laboratory of Grassland Resources, Ministry of Education, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010019, ChinaThis study aimed to characterize the fungal diversity and mycotoxin concentrations of native grass hay in various storage periods. In the present study, the native grass hay samples were collected when stored for 0 d (D0 group), 30 d (D30 group), and 150 d (D150 group), respectively. Here, mycotoxin analyses combined with ITS gene sequence were performed to reveal the changes in response to the storage period. There were notable differences in deoxynivalenol and aflatoxin B<sub>1</sub> concentrations among the three groups. Compared to the D150 group, the diversity of the fungal community was higher in the D0 and D30 groups, which indicating the diversity was significantly influenced by the storage period. No significant (<i>p</i> > 0.05) difference was observed among the three groups on the dominant phyla. Interestingly, a significant (<i>p</i> < 0.05) difference was also observed in <i>Chactomella</i> and <i>Aspergillus</i> among the three groups, the abundance of the <i>Chactomella</i> was significantly (<i>p</i> < 0.05) decreased and the abundance of <i>Aspergillus</i> was statistically (<i>p</i> < 0.05) increased in the D150 group. Correlation analysis of the association of fungi with mycotoxin could provide a comprehensive understanding of the structure and function of the fungal community. These results indicated that the good practices of storage are essential for the prevention of mycotoxin. The information contained in the present study is vital for the further development of strategies for hay storage with high quality in the harsh Mongolian Plateau ecosystem.https://www.mdpi.com/2076-2607/10/6/1154native grass haymycotoxinfungal diversitystorage period |
| spellingShingle | Shuai Du Sihan You Xiaowei Jiang Yuyu Li Yushan Jia Longitudinal Investigation of the Native Grass Hay from Storage to Market Reveals Mycotoxin-Associated Fungi Microorganisms native grass hay mycotoxin fungal diversity storage period |
| title | Longitudinal Investigation of the Native Grass Hay from Storage to Market Reveals Mycotoxin-Associated Fungi |
| title_full | Longitudinal Investigation of the Native Grass Hay from Storage to Market Reveals Mycotoxin-Associated Fungi |
| title_fullStr | Longitudinal Investigation of the Native Grass Hay from Storage to Market Reveals Mycotoxin-Associated Fungi |
| title_full_unstemmed | Longitudinal Investigation of the Native Grass Hay from Storage to Market Reveals Mycotoxin-Associated Fungi |
| title_short | Longitudinal Investigation of the Native Grass Hay from Storage to Market Reveals Mycotoxin-Associated Fungi |
| title_sort | longitudinal investigation of the native grass hay from storage to market reveals mycotoxin associated fungi |
| topic | native grass hay mycotoxin fungal diversity storage period |
| url | https://www.mdpi.com/2076-2607/10/6/1154 |
| work_keys_str_mv | AT shuaidu longitudinalinvestigationofthenativegrasshayfromstoragetomarketrevealsmycotoxinassociatedfungi AT sihanyou longitudinalinvestigationofthenativegrasshayfromstoragetomarketrevealsmycotoxinassociatedfungi AT xiaoweijiang longitudinalinvestigationofthenativegrasshayfromstoragetomarketrevealsmycotoxinassociatedfungi AT yuyuli longitudinalinvestigationofthenativegrasshayfromstoragetomarketrevealsmycotoxinassociatedfungi AT yushanjia longitudinalinvestigationofthenativegrasshayfromstoragetomarketrevealsmycotoxinassociatedfungi |