Mineral trioxide aggregate induces osteoblastogenesis via Atf6
Mineral trioxide aggregate (MTA) has been recommended for various uses in endodontics. To understand the effects of MTA on alveolar bone, we examined whether MTA induces osteoblastic differentiation using MC3T3-E1 cells. MTA enhanced mineralization concomitant with alkaline phosphatase activity in a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Bone Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352187215000078 |
| _version_ | 1811202350196457472 |
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| author | Toyonobu Maeda Atsuko Suzuki Satoshi Yuzawa Yuh Baba Yuichi Kimura Yasumasa Kato |
| author_facet | Toyonobu Maeda Atsuko Suzuki Satoshi Yuzawa Yuh Baba Yuichi Kimura Yasumasa Kato |
| author_sort | Toyonobu Maeda |
| collection | DOAJ |
| description | Mineral trioxide aggregate (MTA) has been recommended for various uses in endodontics. To understand the effects of MTA on alveolar bone, we examined whether MTA induces osteoblastic differentiation using MC3T3-E1 cells. MTA enhanced mineralization concomitant with alkaline phosphatase activity in a dose- and time-dependent manner. MTA increased production of collagens (Type I and Type III) and matrix metalloproteinases (MMP-9 and MMP-13), suggesting that MTA affects bone matrix remodeling. MTA also induced Bglap (osteocalcin) but not Bmp2 (bone morphogenetic protein-2) mRNA expression. We observed induction of Atf6 (activating transcription factor 6, an endoplasmic reticulum (ER) stress response transcription factor) mRNA expression and activation of Atf6 by MTA treatment. Forced expression of p50Atf6 (active form of Atf6) markedly enhanced Bglap mRNA expression. Chromatin immunoprecipitation assay was performed to investigate the increase in p50Atf6 binding to the Bglap promoter region by MTA treatment. Furthermore, knockdown of Atf6 gene expression by introduction of Tet-on Atf6 shRNA expression vector abrogated MTA-induced mineralization. These results suggest that MTA induces in vitro osteoblastogenesis through the Atf6–osteocalcin axis as ER stress signaling. Therefore, MTA in endodontic treatment may affect alveolar bone healing in the resorbed region caused by pulpal infection. |
| first_indexed | 2024-04-12T02:38:21Z |
| format | Article |
| id | doaj.art-317aa60129974a34943d2c1b67506488 |
| institution | Directory Open Access Journal |
| issn | 2352-1872 |
| language | English |
| last_indexed | 2024-04-12T02:38:21Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Bone Reports |
| spelling | doaj.art-317aa60129974a34943d2c1b675064882022-12-22T03:51:24ZengElsevierBone Reports2352-18722015-06-012C364310.1016/j.bonr.2015.03.003Mineral trioxide aggregate induces osteoblastogenesis via Atf6Toyonobu Maeda0Atsuko Suzuki1Satoshi Yuzawa2Yuh Baba3Yuichi Kimura4Yasumasa Kato5Department of Oral Function and Molecular Biology, Ohu University School of Dentistry, Koriyama 963-8611, JapanDepartment of Oral Function and Molecular Biology, Ohu University School of Dentistry, Koriyama 963-8611, JapanDepartment of Oral Function and Molecular Biology, Ohu University School of Dentistry, Koriyama 963-8611, JapanDepartment of General Clinical Medicine, Ohu University School of Dentistry, Koriyama 963-8611, JapanDivision of Endodontics, Department of Conservative Dentistry, Ohu University School of Dentistry, Koriyama 963-8611, JapanDepartment of Oral Function and Molecular Biology, Ohu University School of Dentistry, Koriyama 963-8611, JapanMineral trioxide aggregate (MTA) has been recommended for various uses in endodontics. To understand the effects of MTA on alveolar bone, we examined whether MTA induces osteoblastic differentiation using MC3T3-E1 cells. MTA enhanced mineralization concomitant with alkaline phosphatase activity in a dose- and time-dependent manner. MTA increased production of collagens (Type I and Type III) and matrix metalloproteinases (MMP-9 and MMP-13), suggesting that MTA affects bone matrix remodeling. MTA also induced Bglap (osteocalcin) but not Bmp2 (bone morphogenetic protein-2) mRNA expression. We observed induction of Atf6 (activating transcription factor 6, an endoplasmic reticulum (ER) stress response transcription factor) mRNA expression and activation of Atf6 by MTA treatment. Forced expression of p50Atf6 (active form of Atf6) markedly enhanced Bglap mRNA expression. Chromatin immunoprecipitation assay was performed to investigate the increase in p50Atf6 binding to the Bglap promoter region by MTA treatment. Furthermore, knockdown of Atf6 gene expression by introduction of Tet-on Atf6 shRNA expression vector abrogated MTA-induced mineralization. These results suggest that MTA induces in vitro osteoblastogenesis through the Atf6–osteocalcin axis as ER stress signaling. Therefore, MTA in endodontic treatment may affect alveolar bone healing in the resorbed region caused by pulpal infection.http://www.sciencedirect.com/science/article/pii/S2352187215000078OsteoblastgenesisEndodonticsBone regenerationMineral trioxide aggregateAtf6 |
| spellingShingle | Toyonobu Maeda Atsuko Suzuki Satoshi Yuzawa Yuh Baba Yuichi Kimura Yasumasa Kato Mineral trioxide aggregate induces osteoblastogenesis via Atf6 Bone Reports Osteoblastgenesis Endodontics Bone regeneration Mineral trioxide aggregate Atf6 |
| title | Mineral trioxide aggregate induces osteoblastogenesis via Atf6 |
| title_full | Mineral trioxide aggregate induces osteoblastogenesis via Atf6 |
| title_fullStr | Mineral trioxide aggregate induces osteoblastogenesis via Atf6 |
| title_full_unstemmed | Mineral trioxide aggregate induces osteoblastogenesis via Atf6 |
| title_short | Mineral trioxide aggregate induces osteoblastogenesis via Atf6 |
| title_sort | mineral trioxide aggregate induces osteoblastogenesis via atf6 |
| topic | Osteoblastgenesis Endodontics Bone regeneration Mineral trioxide aggregate Atf6 |
| url | http://www.sciencedirect.com/science/article/pii/S2352187215000078 |
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