Decreased Levels of Thioredoxin <i>o1</i> Influences Stomatal Development and Aperture but Not Photosynthesis under Non-Stress and Saline Conditions
Salinity has a negative impact on plant growth, with photosynthesis being downregulated partially due to osmotic effect and enhanced cellular oxidation. Redox signaling contributes to the plant response playing thioredoxins (TRXs) a central role. In this work we explore the potential contribution of...
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/3/1063 |
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| author | Antonio Sánchez-Guerrero Miquel Nadal Igor Florez-Sarasa Miquel Ribas-Carbó José G. Vallarino Sabrina De Brasi-Velasco Alisdair R. Fernie Jaume Flexas Ana Jiménez Francisca Sevilla |
| author_facet | Antonio Sánchez-Guerrero Miquel Nadal Igor Florez-Sarasa Miquel Ribas-Carbó José G. Vallarino Sabrina De Brasi-Velasco Alisdair R. Fernie Jaume Flexas Ana Jiménez Francisca Sevilla |
| author_sort | Antonio Sánchez-Guerrero |
| collection | DOAJ |
| description | Salinity has a negative impact on plant growth, with photosynthesis being downregulated partially due to osmotic effect and enhanced cellular oxidation. Redox signaling contributes to the plant response playing thioredoxins (TRXs) a central role. In this work we explore the potential contribution of Arabidopsis TRX<i>o</i>1 to the photosynthetic response under salinity analyzing Arabidopsis wild-type (WT) and two <i>Attrxo1</i> mutant lines in their growth under short photoperiod and higher light intensity than previous reported works. Stomatal development and apertures and the antioxidant, hormonal and metabolic acclimation are also analyzed. In control conditions mutant plants displayed less and larger developed stomata and higher pore size which could underlie their higher stomatal conductance, without being affected in other photosynthetic parameters. Under salinity, all genotypes displayed a general decrease in photosynthesis and the oxidative status in the <i>Attrxo1</i> mutant lines was altered, with higher levels of H<sub>2</sub>O<sub>2</sub> and NO but also higher ascorbate/glutathione (ASC/GSH) redox states than WT plants. Finally, sugar changes and increases in abscisic acid (ABA) and NO may be involved in the observed higher stomatal response of the TRX<i>o</i>1-altered plants. Therefore, the lack of <i>AtTRXo1</i> affected stomata development and opening and the mutants modulate their antioxidant, metabolic and hormonal responses to optimize their adaptation to salinity. |
| first_indexed | 2024-03-09T04:03:23Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:03:23Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-317e56c8dc1b4c8f980e1cd4cecb2f632023-12-03T14:10:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-01223106310.3390/ijms22031063Decreased Levels of Thioredoxin <i>o1</i> Influences Stomatal Development and Aperture but Not Photosynthesis under Non-Stress and Saline ConditionsAntonio Sánchez-Guerrero0Miquel Nadal1Igor Florez-Sarasa2Miquel Ribas-Carbó3José G. Vallarino4Sabrina De Brasi-Velasco5Alisdair R. Fernie6Jaume Flexas7Ana Jiménez8Francisca Sevilla9Department of Stress Biology and Plant Pathology, CEBAS-CSIC, 30100 Murcia, SpainGrup de Recerca en Biologia de les Plantes en Condicions Mediterranies, Universitat de les Illes Balears, 07122 Palma de Mallorca, SpainCenter for Research in Agricultural Genomics (CRAG)- CSIC-IRTA-UAB-UB, Campus University of Barcelona, 08193 Barcelona, SpainGrup de Recerca en Biologia de les Plantes en Condicions Mediterranies, Universitat de les Illes Balears, 07122 Palma de Mallorca, SpainMax Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, GermanyDepartment of Stress Biology and Plant Pathology, CEBAS-CSIC, 30100 Murcia, SpainMax Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, GermanyGrup de Recerca en Biologia de les Plantes en Condicions Mediterranies, Universitat de les Illes Balears, 07122 Palma de Mallorca, SpainDepartment of Stress Biology and Plant Pathology, CEBAS-CSIC, 30100 Murcia, SpainDepartment of Stress Biology and Plant Pathology, CEBAS-CSIC, 30100 Murcia, SpainSalinity has a negative impact on plant growth, with photosynthesis being downregulated partially due to osmotic effect and enhanced cellular oxidation. Redox signaling contributes to the plant response playing thioredoxins (TRXs) a central role. In this work we explore the potential contribution of Arabidopsis TRX<i>o</i>1 to the photosynthetic response under salinity analyzing Arabidopsis wild-type (WT) and two <i>Attrxo1</i> mutant lines in their growth under short photoperiod and higher light intensity than previous reported works. Stomatal development and apertures and the antioxidant, hormonal and metabolic acclimation are also analyzed. In control conditions mutant plants displayed less and larger developed stomata and higher pore size which could underlie their higher stomatal conductance, without being affected in other photosynthetic parameters. Under salinity, all genotypes displayed a general decrease in photosynthesis and the oxidative status in the <i>Attrxo1</i> mutant lines was altered, with higher levels of H<sub>2</sub>O<sub>2</sub> and NO but also higher ascorbate/glutathione (ASC/GSH) redox states than WT plants. Finally, sugar changes and increases in abscisic acid (ABA) and NO may be involved in the observed higher stomatal response of the TRX<i>o</i>1-altered plants. Therefore, the lack of <i>AtTRXo1</i> affected stomata development and opening and the mutants modulate their antioxidant, metabolic and hormonal responses to optimize their adaptation to salinity.https://www.mdpi.com/1422-0067/22/3/1063antioxidantsoxidative stressphotosynthesissalinitystomatathioredoxin <i>o</i>1 |
| spellingShingle | Antonio Sánchez-Guerrero Miquel Nadal Igor Florez-Sarasa Miquel Ribas-Carbó José G. Vallarino Sabrina De Brasi-Velasco Alisdair R. Fernie Jaume Flexas Ana Jiménez Francisca Sevilla Decreased Levels of Thioredoxin <i>o1</i> Influences Stomatal Development and Aperture but Not Photosynthesis under Non-Stress and Saline Conditions International Journal of Molecular Sciences antioxidants oxidative stress photosynthesis salinity stomata thioredoxin <i>o</i>1 |
| title | Decreased Levels of Thioredoxin <i>o1</i> Influences Stomatal Development and Aperture but Not Photosynthesis under Non-Stress and Saline Conditions |
| title_full | Decreased Levels of Thioredoxin <i>o1</i> Influences Stomatal Development and Aperture but Not Photosynthesis under Non-Stress and Saline Conditions |
| title_fullStr | Decreased Levels of Thioredoxin <i>o1</i> Influences Stomatal Development and Aperture but Not Photosynthesis under Non-Stress and Saline Conditions |
| title_full_unstemmed | Decreased Levels of Thioredoxin <i>o1</i> Influences Stomatal Development and Aperture but Not Photosynthesis under Non-Stress and Saline Conditions |
| title_short | Decreased Levels of Thioredoxin <i>o1</i> Influences Stomatal Development and Aperture but Not Photosynthesis under Non-Stress and Saline Conditions |
| title_sort | decreased levels of thioredoxin i o1 i influences stomatal development and aperture but not photosynthesis under non stress and saline conditions |
| topic | antioxidants oxidative stress photosynthesis salinity stomata thioredoxin <i>o</i>1 |
| url | https://www.mdpi.com/1422-0067/22/3/1063 |
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