The Effects of Polydeoxyribonucleotide on the Survival of Random Pattern Skin Flaps in Rats
Background Partial or complete necrosis of a skin flap is a common problem. Polydeoxyribonucleotide (PDRN) can be extracted from trout sperm and used as a tissue repair agent. The aim of this study was to investigate whether PDRN could improve the survival of random pattern skin flaps in rats....
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| Format: | Article |
| Language: | English |
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Thieme Medical Publishers, Inc.
2013-05-01
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| Series: | Archives of Plastic Surgery |
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| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.5999/aps.2013.40.3.181 |
| _version_ | 1817989042367627264 |
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| author | Kun Il Chung Han Koo Kim Woo Seob Kim Tae Hui Bae |
| author_facet | Kun Il Chung Han Koo Kim Woo Seob Kim Tae Hui Bae |
| author_sort | Kun Il Chung |
| collection | DOAJ |
| description | Background Partial or complete necrosis of a skin flap is a common problem. Polydeoxyribonucleotide (PDRN) can be extracted from trout sperm and used as a tissue repair agent. The aim of this study was to investigate whether PDRN could improve the survival of random pattern skin flaps in rats.
Methods Twenty-two male Sprague-Dawley rats were randomly divided into two groups: the PDRN treatment group (n=11) and the control group (n=11). Caudally pedicled random pattern skin flaps were elevated on their dorsal skin and resutured. The treatment group received daily intraperitoneal administration of PDRN (8 mg/kg/day), and the control group received fluid vehicle (NaCl 0.9%, 8 mg/kg/day) from day 0 to day 6. On day 7, the flap survival was evaluated and the harvested tissue surrounding the demarcation line of the necrotic area was stained with H&E, anti-rat vascular endothelial cell growth factor (VEGF) antibody, and PECAM-1/CD31 antibody.
Results The average necrotic area of the flap in the PDRN group was significantly smaller when compared with that of the control group. Histologic and immunohistochemical evaluation showed that granulation thickness score and VEGF-positive staining cells were marked higher in the PDRN group than in the control group. PECAM-1/CD31-positive microvascular densities were significantly higher in the PDRN group when compared with the control group.
Conclusions This study confirms that PDRN improves the survival of random pattern skin flaps in rats. These results may represent a new therapeutic approach to enhancing flap viability and achieving faster wound repair. |
| first_indexed | 2024-04-14T00:41:19Z |
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| institution | Directory Open Access Journal |
| issn | 2234-6163 2234-6171 |
| language | English |
| last_indexed | 2024-04-14T00:41:19Z |
| publishDate | 2013-05-01 |
| publisher | Thieme Medical Publishers, Inc. |
| record_format | Article |
| series | Archives of Plastic Surgery |
| spelling | doaj.art-317fe3f1660b4937a49745859dc728ec2022-12-22T02:22:10ZengThieme Medical Publishers, Inc.Archives of Plastic Surgery2234-61632234-61712013-05-01400318118610.5999/aps.2013.40.3.181The Effects of Polydeoxyribonucleotide on the Survival of Random Pattern Skin Flaps in RatsKun Il Chung0Han Koo Kim1Woo Seob Kim2Tae Hui Bae3Department of Plastic and Reconstructive Surgery, Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul, KoreaDepartment of Plastic and Reconstructive Surgery, Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul, KoreaDepartment of Plastic and Reconstructive Surgery, Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul, KoreaDepartment of Plastic and Reconstructive Surgery, Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul, KoreaBackground Partial or complete necrosis of a skin flap is a common problem. Polydeoxyribonucleotide (PDRN) can be extracted from trout sperm and used as a tissue repair agent. The aim of this study was to investigate whether PDRN could improve the survival of random pattern skin flaps in rats. Methods Twenty-two male Sprague-Dawley rats were randomly divided into two groups: the PDRN treatment group (n=11) and the control group (n=11). Caudally pedicled random pattern skin flaps were elevated on their dorsal skin and resutured. The treatment group received daily intraperitoneal administration of PDRN (8 mg/kg/day), and the control group received fluid vehicle (NaCl 0.9%, 8 mg/kg/day) from day 0 to day 6. On day 7, the flap survival was evaluated and the harvested tissue surrounding the demarcation line of the necrotic area was stained with H&E, anti-rat vascular endothelial cell growth factor (VEGF) antibody, and PECAM-1/CD31 antibody. Results The average necrotic area of the flap in the PDRN group was significantly smaller when compared with that of the control group. Histologic and immunohistochemical evaluation showed that granulation thickness score and VEGF-positive staining cells were marked higher in the PDRN group than in the control group. PECAM-1/CD31-positive microvascular densities were significantly higher in the PDRN group when compared with the control group. Conclusions This study confirms that PDRN improves the survival of random pattern skin flaps in rats. These results may represent a new therapeutic approach to enhancing flap viability and achieving faster wound repair.http://www.thieme-connect.de/DOI/DOI?10.5999/aps.2013.40.3.181polydeoxyribonucleotidessurgical flapsangiogenesis modulating agentsvascular endothelial growth factorsantigens, cd31 |
| spellingShingle | Kun Il Chung Han Koo Kim Woo Seob Kim Tae Hui Bae The Effects of Polydeoxyribonucleotide on the Survival of Random Pattern Skin Flaps in Rats Archives of Plastic Surgery polydeoxyribonucleotides surgical flaps angiogenesis modulating agents vascular endothelial growth factors antigens, cd31 |
| title | The Effects of Polydeoxyribonucleotide on the Survival of Random Pattern Skin Flaps in Rats |
| title_full | The Effects of Polydeoxyribonucleotide on the Survival of Random Pattern Skin Flaps in Rats |
| title_fullStr | The Effects of Polydeoxyribonucleotide on the Survival of Random Pattern Skin Flaps in Rats |
| title_full_unstemmed | The Effects of Polydeoxyribonucleotide on the Survival of Random Pattern Skin Flaps in Rats |
| title_short | The Effects of Polydeoxyribonucleotide on the Survival of Random Pattern Skin Flaps in Rats |
| title_sort | effects of polydeoxyribonucleotide on the survival of random pattern skin flaps in rats |
| topic | polydeoxyribonucleotides surgical flaps angiogenesis modulating agents vascular endothelial growth factors antigens, cd31 |
| url | http://www.thieme-connect.de/DOI/DOI?10.5999/aps.2013.40.3.181 |
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