Comparison of Two Diagnostic Assays for the Detection of Serum Neutralizing Antibody to Porcine Epidemic Diarrhea Virus
Lactogenic immunity is important for the protection of piglets against many pathogens including porcine epidemic diarrhea virus. Circulating neutralizing antibodies levels in sow sera may help determine if a detectable immune response could confer protection to piglets. Neutralizing antibodies can b...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/4/757 |
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| author | Justin Brown Korakrit Poonsuk Ting-Yu Cheng Chris Rademacher Erin Kalkwarf Liying Tian Lauren A. McKeen Chong Wang Luis Gimenez-Lirola David Baum Locke A. Karriker |
| author_facet | Justin Brown Korakrit Poonsuk Ting-Yu Cheng Chris Rademacher Erin Kalkwarf Liying Tian Lauren A. McKeen Chong Wang Luis Gimenez-Lirola David Baum Locke A. Karriker |
| author_sort | Justin Brown |
| collection | DOAJ |
| description | Lactogenic immunity is important for the protection of piglets against many pathogens including porcine epidemic diarrhea virus. Circulating neutralizing antibodies levels in sow sera may help determine if a detectable immune response could confer protection to piglets. Neutralizing antibodies can be detected through various diagnostic assays. This study evaluated the diagnostic characteristics of two neutralizing antibody assays for porcine epidemic diarrhea virus neutralizing antibodies in serum of challenged gilts. Four treatment groups, control, non-vaccinated, vaccinated prior to challenge, and vaccinated following challenge, were comprised of 20 gilts. Serum sample were collected from each gilt prior to and following challenge with porcine epidemic diarrhea virus. Samples were evaluated for the presence of neutralizing antibodies via a fluorescent focus neutralization assay and a high-throughput neutralization assay. Diagnostic sensitivity and specificity for the fluorescent focus neutralization and high-throughput neutralization assays for this study were optimized at a cutoff of a dilution of 80 and 80% fluorescent reduction respectively and demonstrated moderate agreement based off the kappa statistic. The focus fluorescent neutralization and high-throughput neutralization assays can be used to monitor the status of neutralizing antibodies within animals or a population of animals. The high-throughput assay has advantages over the focus fluorescent assay in that it has a higher specificity at the indicated cut-off and the nature of the results allows for more discrimination between individual results. |
| first_indexed | 2024-03-11T09:15:47Z |
| format | Article |
| id | doaj.art-31818917f418415a83f447e9b11404a8 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T09:15:47Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-31818917f418415a83f447e9b11404a82023-11-16T18:41:10ZengMDPI AGAnimals2076-26152023-02-0113475710.3390/ani13040757Comparison of Two Diagnostic Assays for the Detection of Serum Neutralizing Antibody to Porcine Epidemic Diarrhea VirusJustin Brown0Korakrit Poonsuk1Ting-Yu Cheng2Chris Rademacher3Erin Kalkwarf4Liying Tian5Lauren A. McKeen6Chong Wang7Luis Gimenez-Lirola8David Baum9Locke A. Karriker10Swine Medicine Education Center, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USANebraska Veterinary Diagnostic Center, University of Nebraska-Lincoln, Lincoln, NE 68588, USAVeterinary Preventive Medicine, College of Veterinary Medicine, Ohio State University, Columbus, OH 43210, USAVeterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USAVeterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USAVeterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Statistics, Iowa State University, 1121 Snedecor Hall, Ames, IA 50011, USADepartment of Statistics, Iowa State University, 1121 Snedecor Hall, Ames, IA 50011, USAVeterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USAVeterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USASwine Medicine Education Center, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USALactogenic immunity is important for the protection of piglets against many pathogens including porcine epidemic diarrhea virus. Circulating neutralizing antibodies levels in sow sera may help determine if a detectable immune response could confer protection to piglets. Neutralizing antibodies can be detected through various diagnostic assays. This study evaluated the diagnostic characteristics of two neutralizing antibody assays for porcine epidemic diarrhea virus neutralizing antibodies in serum of challenged gilts. Four treatment groups, control, non-vaccinated, vaccinated prior to challenge, and vaccinated following challenge, were comprised of 20 gilts. Serum sample were collected from each gilt prior to and following challenge with porcine epidemic diarrhea virus. Samples were evaluated for the presence of neutralizing antibodies via a fluorescent focus neutralization assay and a high-throughput neutralization assay. Diagnostic sensitivity and specificity for the fluorescent focus neutralization and high-throughput neutralization assays for this study were optimized at a cutoff of a dilution of 80 and 80% fluorescent reduction respectively and demonstrated moderate agreement based off the kappa statistic. The focus fluorescent neutralization and high-throughput neutralization assays can be used to monitor the status of neutralizing antibodies within animals or a population of animals. The high-throughput assay has advantages over the focus fluorescent assay in that it has a higher specificity at the indicated cut-off and the nature of the results allows for more discrimination between individual results.https://www.mdpi.com/2076-2615/13/4/757PEDVneutralizing antibodydiagnostic assay |
| spellingShingle | Justin Brown Korakrit Poonsuk Ting-Yu Cheng Chris Rademacher Erin Kalkwarf Liying Tian Lauren A. McKeen Chong Wang Luis Gimenez-Lirola David Baum Locke A. Karriker Comparison of Two Diagnostic Assays for the Detection of Serum Neutralizing Antibody to Porcine Epidemic Diarrhea Virus Animals PEDV neutralizing antibody diagnostic assay |
| title | Comparison of Two Diagnostic Assays for the Detection of Serum Neutralizing Antibody to Porcine Epidemic Diarrhea Virus |
| title_full | Comparison of Two Diagnostic Assays for the Detection of Serum Neutralizing Antibody to Porcine Epidemic Diarrhea Virus |
| title_fullStr | Comparison of Two Diagnostic Assays for the Detection of Serum Neutralizing Antibody to Porcine Epidemic Diarrhea Virus |
| title_full_unstemmed | Comparison of Two Diagnostic Assays for the Detection of Serum Neutralizing Antibody to Porcine Epidemic Diarrhea Virus |
| title_short | Comparison of Two Diagnostic Assays for the Detection of Serum Neutralizing Antibody to Porcine Epidemic Diarrhea Virus |
| title_sort | comparison of two diagnostic assays for the detection of serum neutralizing antibody to porcine epidemic diarrhea virus |
| topic | PEDV neutralizing antibody diagnostic assay |
| url | https://www.mdpi.com/2076-2615/13/4/757 |
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