Determination of 27 Glucocorticoids in Urine by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry Using UniSpray<sup>TM</sup> Source
Glucocorticoids (GCs) are a group of the most important and commonly used anti-inflammatory, antiallergenic, and immunosuppressive drugs. Like narcotics, they can be addictive if taken at increasing doses to achieve greater analgesic effects. The purpose of the study was to develop initial and confi...
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MDPI AG
2023-02-01
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| Online Access: | https://www.mdpi.com/2297-8739/10/3/155 |
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| author | Mariola Wicka Krzysztof Grucza Aleksandra Drapała Patryk Konarski Dorota Kwiatkowska |
| author_facet | Mariola Wicka Krzysztof Grucza Aleksandra Drapała Patryk Konarski Dorota Kwiatkowska |
| author_sort | Mariola Wicka |
| collection | DOAJ |
| description | Glucocorticoids (GCs) are a group of the most important and commonly used anti-inflammatory, antiallergenic, and immunosuppressive drugs. Like narcotics, they can be addictive if taken at increasing doses to achieve greater analgesic effects. The purpose of the study was to develop initial and confirmation testing analytical methods that would allow for the identification of glucocorticoid class substances in human urine to be used for routine analyses for the purposes of prosecution in the case of abuse, for clinical toxicology, medical jurisprudence, as well as for routine testing of athletes for the presence of prohibited substances in sports by means of ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) using a new generation ionization source UniSprayTM (U.S). This new method allows for the simultaneous detection of 27 glucocorticoids in human urine using LC-MS/MS. The tests conducted yielded relatively low LOD and LOQ values, ranging from 0.06 ng/mL to 0.14 ng/mL and 0.75 ng/mL for LOD and LOQ, respectively. |
| first_indexed | 2024-03-11T05:55:07Z |
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| id | doaj.art-31828e410115442e81c1ff2084e8e1dc |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-11T05:55:07Z |
| publishDate | 2023-02-01 |
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| spelling | doaj.art-31828e410115442e81c1ff2084e8e1dc2023-11-17T13:49:49ZengMDPI AGSeparations2297-87392023-02-0110315510.3390/separations10030155Determination of 27 Glucocorticoids in Urine by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry Using UniSpray<sup>TM</sup> SourceMariola Wicka0Krzysztof Grucza1Aleksandra Drapała2Patryk Konarski3Dorota Kwiatkowska4Polish Anti-Doping Laboratory, Ksiecia Ziemowita 53 bud. 4, 03-885 Warsaw, PolandPolish Anti-Doping Laboratory, Ksiecia Ziemowita 53 bud. 4, 03-885 Warsaw, PolandPolish Anti-Doping Laboratory, Ksiecia Ziemowita 53 bud. 4, 03-885 Warsaw, PolandPolish Anti-Doping Laboratory, Ksiecia Ziemowita 53 bud. 4, 03-885 Warsaw, PolandPolish Anti-Doping Laboratory, Ksiecia Ziemowita 53 bud. 4, 03-885 Warsaw, PolandGlucocorticoids (GCs) are a group of the most important and commonly used anti-inflammatory, antiallergenic, and immunosuppressive drugs. Like narcotics, they can be addictive if taken at increasing doses to achieve greater analgesic effects. The purpose of the study was to develop initial and confirmation testing analytical methods that would allow for the identification of glucocorticoid class substances in human urine to be used for routine analyses for the purposes of prosecution in the case of abuse, for clinical toxicology, medical jurisprudence, as well as for routine testing of athletes for the presence of prohibited substances in sports by means of ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) using a new generation ionization source UniSprayTM (U.S). This new method allows for the simultaneous detection of 27 glucocorticoids in human urine using LC-MS/MS. The tests conducted yielded relatively low LOD and LOQ values, ranging from 0.06 ng/mL to 0.14 ng/mL and 0.75 ng/mL for LOD and LOQ, respectively.https://www.mdpi.com/2297-8739/10/3/155glucocorticoidsUHPLC-MS/MSdopingurine |
| spellingShingle | Mariola Wicka Krzysztof Grucza Aleksandra Drapała Patryk Konarski Dorota Kwiatkowska Determination of 27 Glucocorticoids in Urine by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry Using UniSpray<sup>TM</sup> Source Separations glucocorticoids UHPLC-MS/MS doping urine |
| title | Determination of 27 Glucocorticoids in Urine by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry Using UniSpray<sup>TM</sup> Source |
| title_full | Determination of 27 Glucocorticoids in Urine by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry Using UniSpray<sup>TM</sup> Source |
| title_fullStr | Determination of 27 Glucocorticoids in Urine by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry Using UniSpray<sup>TM</sup> Source |
| title_full_unstemmed | Determination of 27 Glucocorticoids in Urine by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry Using UniSpray<sup>TM</sup> Source |
| title_short | Determination of 27 Glucocorticoids in Urine by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry Using UniSpray<sup>TM</sup> Source |
| title_sort | determination of 27 glucocorticoids in urine by ultra high performance liquid chromatography tandem mass spectrometry using unispray sup tm sup source |
| topic | glucocorticoids UHPLC-MS/MS doping urine |
| url | https://www.mdpi.com/2297-8739/10/3/155 |
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