Impact of Rumen Fluid Storage on In Vitro Feed Fermentation Characteristics
Storing rumen fluid (RF) has the potential to standardize subsequent in vitro feed fermentation studies. The first phase of this experiment aimed to evaluate the effect of two RF storage methods on gas composition and dry matter disappearance (DMD) in wheat grain and lucerne hay under in vitro ferme...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/9/4/392 |
| _version_ | 1797605492313292800 |
|---|---|
| author | Bereket Zeleke Tunkala Kristy DiGiacomo Pablo S. Alvarez Hess Frank R. Dunshea Brian J. Leury |
| author_facet | Bereket Zeleke Tunkala Kristy DiGiacomo Pablo S. Alvarez Hess Frank R. Dunshea Brian J. Leury |
| author_sort | Bereket Zeleke Tunkala |
| collection | DOAJ |
| description | Storing rumen fluid (RF) has the potential to standardize subsequent in vitro feed fermentation studies. The first phase of this experiment aimed to evaluate the effect of two RF storage methods on gas composition and dry matter disappearance (DMD) in wheat grain and lucerne hay under in vitro fermentation. The storage methods were as follows: (1) snap-freezing RF using liquid nitrogen and then storing it at −80 °C (−80 °C); and (2) mixing RF with 5% dimethyl sulfoxide (DMSO), subsequently freezing it at −20 °C (D−20 °C), and comparing it to fresh RF on days 1, 14, and 30 post collection. The objective of the second phase was to quantify the impact of preserving the RF for 180 days at D−20 °C on the in vitro fermentation parameters. The methane composition was lower (<i>p</i> < 0.001) in both the preserved RFs than in the fresh RF. There was no difference (<i>p</i> < 0.05) in DMD values between days 14 and 30. The average cumulative gas production and DMD from the RF stored at D−20 °C was higher than that from the RF stored at −80 °C. Moreover, there was no difference between day 30 and day 180 in the total gas production and lag time when fermenting with RF preserved at D−20 °C. Therefore, storing RF at D−20 °C is preferable to storing it at −80 °C when access to fresh RF is limited. |
| first_indexed | 2024-03-11T05:01:52Z |
| format | Article |
| id | doaj.art-3183145e27d7426381d3222d95ca66ef |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T05:01:52Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-3183145e27d7426381d3222d95ca66ef2023-11-17T19:11:16ZengMDPI AGFermentation2311-56372023-04-019439210.3390/fermentation9040392Impact of Rumen Fluid Storage on In Vitro Feed Fermentation CharacteristicsBereket Zeleke Tunkala0Kristy DiGiacomo1Pablo S. Alvarez Hess2Frank R. Dunshea3Brian J. Leury4Faculty of Science, The University of Melbourne, Parkville, VIC 3010, AustraliaFaculty of Science, The University of Melbourne, Parkville, VIC 3010, AustraliaAgriculture Victoria Research, 1301 Hazeldean Road, Ellinbank, VIC 3821, AustraliaFaculty of Science, The University of Melbourne, Parkville, VIC 3010, AustraliaFaculty of Science, The University of Melbourne, Parkville, VIC 3010, AustraliaStoring rumen fluid (RF) has the potential to standardize subsequent in vitro feed fermentation studies. The first phase of this experiment aimed to evaluate the effect of two RF storage methods on gas composition and dry matter disappearance (DMD) in wheat grain and lucerne hay under in vitro fermentation. The storage methods were as follows: (1) snap-freezing RF using liquid nitrogen and then storing it at −80 °C (−80 °C); and (2) mixing RF with 5% dimethyl sulfoxide (DMSO), subsequently freezing it at −20 °C (D−20 °C), and comparing it to fresh RF on days 1, 14, and 30 post collection. The objective of the second phase was to quantify the impact of preserving the RF for 180 days at D−20 °C on the in vitro fermentation parameters. The methane composition was lower (<i>p</i> < 0.001) in both the preserved RFs than in the fresh RF. There was no difference (<i>p</i> < 0.05) in DMD values between days 14 and 30. The average cumulative gas production and DMD from the RF stored at D−20 °C was higher than that from the RF stored at −80 °C. Moreover, there was no difference between day 30 and day 180 in the total gas production and lag time when fermenting with RF preserved at D−20 °C. Therefore, storing RF at D−20 °C is preferable to storing it at −80 °C when access to fresh RF is limited.https://www.mdpi.com/2311-5637/9/4/392preservationgas productionmethanedry matter disappearancevolatile fatty acids |
| spellingShingle | Bereket Zeleke Tunkala Kristy DiGiacomo Pablo S. Alvarez Hess Frank R. Dunshea Brian J. Leury Impact of Rumen Fluid Storage on In Vitro Feed Fermentation Characteristics Fermentation preservation gas production methane dry matter disappearance volatile fatty acids |
| title | Impact of Rumen Fluid Storage on In Vitro Feed Fermentation Characteristics |
| title_full | Impact of Rumen Fluid Storage on In Vitro Feed Fermentation Characteristics |
| title_fullStr | Impact of Rumen Fluid Storage on In Vitro Feed Fermentation Characteristics |
| title_full_unstemmed | Impact of Rumen Fluid Storage on In Vitro Feed Fermentation Characteristics |
| title_short | Impact of Rumen Fluid Storage on In Vitro Feed Fermentation Characteristics |
| title_sort | impact of rumen fluid storage on in vitro feed fermentation characteristics |
| topic | preservation gas production methane dry matter disappearance volatile fatty acids |
| url | https://www.mdpi.com/2311-5637/9/4/392 |
| work_keys_str_mv | AT bereketzeleketunkala impactofrumenfluidstorageoninvitrofeedfermentationcharacteristics AT kristydigiacomo impactofrumenfluidstorageoninvitrofeedfermentationcharacteristics AT pablosalvarezhess impactofrumenfluidstorageoninvitrofeedfermentationcharacteristics AT frankrdunshea impactofrumenfluidstorageoninvitrofeedfermentationcharacteristics AT brianjleury impactofrumenfluidstorageoninvitrofeedfermentationcharacteristics |