Global Proteome Profiling Revealed the Adaptive Reprogramming of Barley Flag Leaf to Drought and Elevated Temperature
Plants, as sessile organisms, have developed sophisticated mechanisms to survive in changing environments. Recent advances in omics approaches have facilitated the exploration of plant genomes; however, the molecular mechanisms underlying the responses of barley and other cereals to multiple abiotic...
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MDPI AG
2023-06-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/13/1685 |
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| author | Krzysztof Mikołajczak Anetta Kuczyńska Paweł Krajewski Michał Kempa Natalia Witaszak |
| author_facet | Krzysztof Mikołajczak Anetta Kuczyńska Paweł Krajewski Michał Kempa Natalia Witaszak |
| author_sort | Krzysztof Mikołajczak |
| collection | DOAJ |
| description | Plants, as sessile organisms, have developed sophisticated mechanisms to survive in changing environments. Recent advances in omics approaches have facilitated the exploration of plant genomes; however, the molecular mechanisms underlying the responses of barley and other cereals to multiple abiotic stresses remain largely unclear. Exposure to stress stimuli affects many proteins with regulatory and protective functions. In the present study, we employed liquid chromatography coupled with high-resolution mass spectrometry to identify stress-responsive proteins on the genome-wide scale of barley flag leaves exposed to drought, heat, or both. Profound alterations in the proteome of genotypes with different flag leaf sizes were found. The role of stress-inducible proteins was discussed and candidates underlying the universal stress response were proposed, including dehydrins. Moreover, the putative functions of several unknown proteins that can mediate responses to stress stimuli were explored using Pfam annotation, including calmodulin-like proteins. Finally, the confrontation of protein and mRNA abundances was performed. A correlation network between transcripts and proteins performance revealed several components of the stress-adaptive pathways in barley flag leaf. Taking the findings together, promising candidates for improving the tolerance of barley and other cereals to multivariate stresses were uncovered. The presented proteomic landscape and its relationship to transcriptomic remodeling provide novel insights for understanding the molecular responses of plants to environmental cues. |
| first_indexed | 2024-03-11T01:44:57Z |
| format | Article |
| id | doaj.art-31852524b5cc4c109d0bbc9372d127e2 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T01:44:57Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-31852524b5cc4c109d0bbc9372d127e22023-11-18T16:18:34ZengMDPI AGCells2073-44092023-06-011213168510.3390/cells12131685Global Proteome Profiling Revealed the Adaptive Reprogramming of Barley Flag Leaf to Drought and Elevated TemperatureKrzysztof Mikołajczak0Anetta Kuczyńska1Paweł Krajewski2Michał Kempa3Natalia Witaszak4Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznań, PolandInstitute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznań, PolandInstitute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznań, PolandInstitute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznań, PolandInstitute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznań, PolandPlants, as sessile organisms, have developed sophisticated mechanisms to survive in changing environments. Recent advances in omics approaches have facilitated the exploration of plant genomes; however, the molecular mechanisms underlying the responses of barley and other cereals to multiple abiotic stresses remain largely unclear. Exposure to stress stimuli affects many proteins with regulatory and protective functions. In the present study, we employed liquid chromatography coupled with high-resolution mass spectrometry to identify stress-responsive proteins on the genome-wide scale of barley flag leaves exposed to drought, heat, or both. Profound alterations in the proteome of genotypes with different flag leaf sizes were found. The role of stress-inducible proteins was discussed and candidates underlying the universal stress response were proposed, including dehydrins. Moreover, the putative functions of several unknown proteins that can mediate responses to stress stimuli were explored using Pfam annotation, including calmodulin-like proteins. Finally, the confrontation of protein and mRNA abundances was performed. A correlation network between transcripts and proteins performance revealed several components of the stress-adaptive pathways in barley flag leaf. Taking the findings together, promising candidates for improving the tolerance of barley and other cereals to multivariate stresses were uncovered. The presented proteomic landscape and its relationship to transcriptomic remodeling provide novel insights for understanding the molecular responses of plants to environmental cues.https://www.mdpi.com/2073-4409/12/13/1685abiotic stresscombined stressflag leafproteomicsregulatory componentsstress-induced proteins |
| spellingShingle | Krzysztof Mikołajczak Anetta Kuczyńska Paweł Krajewski Michał Kempa Natalia Witaszak Global Proteome Profiling Revealed the Adaptive Reprogramming of Barley Flag Leaf to Drought and Elevated Temperature Cells abiotic stress combined stress flag leaf proteomics regulatory components stress-induced proteins |
| title | Global Proteome Profiling Revealed the Adaptive Reprogramming of Barley Flag Leaf to Drought and Elevated Temperature |
| title_full | Global Proteome Profiling Revealed the Adaptive Reprogramming of Barley Flag Leaf to Drought and Elevated Temperature |
| title_fullStr | Global Proteome Profiling Revealed the Adaptive Reprogramming of Barley Flag Leaf to Drought and Elevated Temperature |
| title_full_unstemmed | Global Proteome Profiling Revealed the Adaptive Reprogramming of Barley Flag Leaf to Drought and Elevated Temperature |
| title_short | Global Proteome Profiling Revealed the Adaptive Reprogramming of Barley Flag Leaf to Drought and Elevated Temperature |
| title_sort | global proteome profiling revealed the adaptive reprogramming of barley flag leaf to drought and elevated temperature |
| topic | abiotic stress combined stress flag leaf proteomics regulatory components stress-induced proteins |
| url | https://www.mdpi.com/2073-4409/12/13/1685 |
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