D-amino acid/gentamicin loaded zwitterionic hydrogel coatings with optimized mechanical stability and biofilm inhibition capabilities
Biofilms associated bacterial infections on material surfaces have become a tremendous biomedical challenge. Developing antimicrobial coatings on biomaterial surfaces and endowing them with decent mechanical stability and biofilm inhibition capabilities is an efficient way to resist bacterial attach...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-04-01
|
| Series: | Frontiers in Materials |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmats.2024.1371351/full |
| _version_ | 1797224049229692928 |
|---|---|
| author | Jingzhi Yang Jingzhi Yang Yami Ran Yami Ran Yami Ran Junsen Zhao Junsen Zhao Taiwei Xing Taiwei Xing Xiangping Hao Xiangping Hao Dawei Zhang Dawei Zhang Dawei Zhang |
| author_facet | Jingzhi Yang Jingzhi Yang Yami Ran Yami Ran Yami Ran Junsen Zhao Junsen Zhao Taiwei Xing Taiwei Xing Xiangping Hao Xiangping Hao Dawei Zhang Dawei Zhang Dawei Zhang |
| author_sort | Jingzhi Yang |
| collection | DOAJ |
| description | Biofilms associated bacterial infections on material surfaces have become a tremendous biomedical challenge. Developing antimicrobial coatings on biomaterial surfaces and endowing them with decent mechanical stability and biofilm inhibition capabilities is an efficient way to resist bacterial attachment and biofilm formation. Herein, we integrated 2-hydroxyethyl methacrylate (HEMA) and D-amino acid mixtures based antibiofilm combinations with sulfobetaine methacrylate (SBMA) hydrogel coatings. The obtained hydrogel coatings demonstrated high stability in various transport and service environments. The proper incorporation of the HEMA achieves only ∼3% weight loss of SBMA hydrogel coatings after swelling, flushing and abrasion damages. In addition, both biofilm formation inhibiting D-amino acid mixtures and bacteria-killing gentamicin components were loaded in the coatings. The synergistic action of these two components was able to significantly reduce the bacterial numbers with up to 2.3 log reduction. The bacteria exposed to D-amino acid mixtures was difficult to form biofilm, which was more susceptive to the harm of gentamicin. This work provides an effective paradigm to integrate mechanically stable SBMA-HEMA hydrogel with natural D-amino acid mixtures based antibiofilm agents to generate biomedical surfaces to combat biofilms associated bacterial infections. |
| first_indexed | 2024-04-24T13:46:56Z |
| format | Article |
| id | doaj.art-318811dd381644bd8fb0a138a90b5b18 |
| institution | Directory Open Access Journal |
| issn | 2296-8016 |
| language | English |
| last_indexed | 2024-04-24T13:46:56Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Materials |
| spelling | doaj.art-318811dd381644bd8fb0a138a90b5b182024-04-04T05:08:45ZengFrontiers Media S.A.Frontiers in Materials2296-80162024-04-011110.3389/fmats.2024.13713511371351D-amino acid/gentamicin loaded zwitterionic hydrogel coatings with optimized mechanical stability and biofilm inhibition capabilitiesJingzhi Yang0Jingzhi Yang1Yami Ran2Yami Ran3Yami Ran4Junsen Zhao5Junsen Zhao6Taiwei Xing7Taiwei Xing8Xiangping Hao9Xiangping Hao10Dawei Zhang11Dawei Zhang12Dawei Zhang13Beijing Advanced Innovation Center for Materials Genome Engineering, Institute for Advanced Materials and Technology, University of Science and Technology Beijing, Beijing, ChinaNational Materials Corrosion and Protection Data Center, University of Science and Technology Beijing, Beijing, ChinaBeijing Advanced Innovation Center for Materials Genome Engineering, Institute for Advanced Materials and Technology, University of Science and Technology Beijing, Beijing, ChinaNational Materials Corrosion and Protection Data Center, University of Science and Technology Beijing, Beijing, ChinaBRI Southeast Asia Network for Corrosion and Protection, Shunde Graduate School of University of Science and Technology Beijing, Foshan, ChinaBeijing Advanced Innovation Center for Materials Genome Engineering, Institute for Advanced Materials and Technology, University of Science and Technology Beijing, Beijing, ChinaNational Materials Corrosion and Protection Data Center, University of Science and Technology Beijing, Beijing, ChinaBeijing Advanced Innovation Center for Materials Genome Engineering, Institute for Advanced Materials and Technology, University of Science and Technology Beijing, Beijing, ChinaNational Materials Corrosion and Protection Data Center, University of Science and Technology Beijing, Beijing, ChinaNational Materials Corrosion and Protection Data Center, University of Science and Technology Beijing, Beijing, ChinaSchool of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, ChinaBeijing Advanced Innovation Center for Materials Genome Engineering, Institute for Advanced Materials and Technology, University of Science and Technology Beijing, Beijing, ChinaNational Materials Corrosion and Protection Data Center, University of Science and Technology Beijing, Beijing, ChinaBRI Southeast Asia Network for Corrosion and Protection, Shunde Graduate School of University of Science and Technology Beijing, Foshan, ChinaBiofilms associated bacterial infections on material surfaces have become a tremendous biomedical challenge. Developing antimicrobial coatings on biomaterial surfaces and endowing them with decent mechanical stability and biofilm inhibition capabilities is an efficient way to resist bacterial attachment and biofilm formation. Herein, we integrated 2-hydroxyethyl methacrylate (HEMA) and D-amino acid mixtures based antibiofilm combinations with sulfobetaine methacrylate (SBMA) hydrogel coatings. The obtained hydrogel coatings demonstrated high stability in various transport and service environments. The proper incorporation of the HEMA achieves only ∼3% weight loss of SBMA hydrogel coatings after swelling, flushing and abrasion damages. In addition, both biofilm formation inhibiting D-amino acid mixtures and bacteria-killing gentamicin components were loaded in the coatings. The synergistic action of these two components was able to significantly reduce the bacterial numbers with up to 2.3 log reduction. The bacteria exposed to D-amino acid mixtures was difficult to form biofilm, which was more susceptive to the harm of gentamicin. This work provides an effective paradigm to integrate mechanically stable SBMA-HEMA hydrogel with natural D-amino acid mixtures based antibiofilm agents to generate biomedical surfaces to combat biofilms associated bacterial infections.https://www.frontiersin.org/articles/10.3389/fmats.2024.1371351/fullzwitterionD-amino acidsantibiofilm coatingsdrug cocktailsmechanical stability |
| spellingShingle | Jingzhi Yang Jingzhi Yang Yami Ran Yami Ran Yami Ran Junsen Zhao Junsen Zhao Taiwei Xing Taiwei Xing Xiangping Hao Xiangping Hao Dawei Zhang Dawei Zhang Dawei Zhang D-amino acid/gentamicin loaded zwitterionic hydrogel coatings with optimized mechanical stability and biofilm inhibition capabilities Frontiers in Materials zwitterion D-amino acids antibiofilm coatings drug cocktails mechanical stability |
| title | D-amino acid/gentamicin loaded zwitterionic hydrogel coatings with optimized mechanical stability and biofilm inhibition capabilities |
| title_full | D-amino acid/gentamicin loaded zwitterionic hydrogel coatings with optimized mechanical stability and biofilm inhibition capabilities |
| title_fullStr | D-amino acid/gentamicin loaded zwitterionic hydrogel coatings with optimized mechanical stability and biofilm inhibition capabilities |
| title_full_unstemmed | D-amino acid/gentamicin loaded zwitterionic hydrogel coatings with optimized mechanical stability and biofilm inhibition capabilities |
| title_short | D-amino acid/gentamicin loaded zwitterionic hydrogel coatings with optimized mechanical stability and biofilm inhibition capabilities |
| title_sort | d amino acid gentamicin loaded zwitterionic hydrogel coatings with optimized mechanical stability and biofilm inhibition capabilities |
| topic | zwitterion D-amino acids antibiofilm coatings drug cocktails mechanical stability |
| url | https://www.frontiersin.org/articles/10.3389/fmats.2024.1371351/full |
| work_keys_str_mv | AT jingzhiyang daminoacidgentamicinloadedzwitterionichydrogelcoatingswithoptimizedmechanicalstabilityandbiofilminhibitioncapabilities AT jingzhiyang daminoacidgentamicinloadedzwitterionichydrogelcoatingswithoptimizedmechanicalstabilityandbiofilminhibitioncapabilities AT yamiran daminoacidgentamicinloadedzwitterionichydrogelcoatingswithoptimizedmechanicalstabilityandbiofilminhibitioncapabilities AT yamiran daminoacidgentamicinloadedzwitterionichydrogelcoatingswithoptimizedmechanicalstabilityandbiofilminhibitioncapabilities AT yamiran daminoacidgentamicinloadedzwitterionichydrogelcoatingswithoptimizedmechanicalstabilityandbiofilminhibitioncapabilities AT junsenzhao daminoacidgentamicinloadedzwitterionichydrogelcoatingswithoptimizedmechanicalstabilityandbiofilminhibitioncapabilities AT junsenzhao daminoacidgentamicinloadedzwitterionichydrogelcoatingswithoptimizedmechanicalstabilityandbiofilminhibitioncapabilities AT taiweixing daminoacidgentamicinloadedzwitterionichydrogelcoatingswithoptimizedmechanicalstabilityandbiofilminhibitioncapabilities AT taiweixing daminoacidgentamicinloadedzwitterionichydrogelcoatingswithoptimizedmechanicalstabilityandbiofilminhibitioncapabilities AT xiangpinghao daminoacidgentamicinloadedzwitterionichydrogelcoatingswithoptimizedmechanicalstabilityandbiofilminhibitioncapabilities AT xiangpinghao daminoacidgentamicinloadedzwitterionichydrogelcoatingswithoptimizedmechanicalstabilityandbiofilminhibitioncapabilities AT daweizhang daminoacidgentamicinloadedzwitterionichydrogelcoatingswithoptimizedmechanicalstabilityandbiofilminhibitioncapabilities AT daweizhang daminoacidgentamicinloadedzwitterionichydrogelcoatingswithoptimizedmechanicalstabilityandbiofilminhibitioncapabilities AT daweizhang daminoacidgentamicinloadedzwitterionichydrogelcoatingswithoptimizedmechanicalstabilityandbiofilminhibitioncapabilities |