Nanoscale organization of two-dimensional multimeric pMHC reagents with DNA origami for CD8+ T cell detection
MHC-peptide multimers are important reagents for detecting antigen specific T cells. Here the authors show that DNA scaffolds can be used to make MHC-peptide multimers and the avidity controlled so that low abundance or T cells with low affinity TCR can be detected using these reagents.
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-31684-8 |
| _version_ | 1811219065325223936 |
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| author | Yueyang Sun Lu Yan Jiajia Sun Mingshu Xiao Wei Lai Guangqi Song Li Li Chunhai Fan Hao Pei |
| author_facet | Yueyang Sun Lu Yan Jiajia Sun Mingshu Xiao Wei Lai Guangqi Song Li Li Chunhai Fan Hao Pei |
| author_sort | Yueyang Sun |
| collection | DOAJ |
| description | MHC-peptide multimers are important reagents for detecting antigen specific T cells. Here the authors show that DNA scaffolds can be used to make MHC-peptide multimers and the avidity controlled so that low abundance or T cells with low affinity TCR can be detected using these reagents. |
| first_indexed | 2024-04-12T07:19:08Z |
| format | Article |
| id | doaj.art-3188c87bd53f4b86ba7bf605cf25bfe5 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-12T07:19:08Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-3188c87bd53f4b86ba7bf605cf25bfe52022-12-22T03:42:22ZengNature PortfolioNature Communications2041-17232022-07-0113111110.1038/s41467-022-31684-8Nanoscale organization of two-dimensional multimeric pMHC reagents with DNA origami for CD8+ T cell detectionYueyang Sun0Lu Yan1Jiajia Sun2Mingshu Xiao3Wei Lai4Guangqi Song5Li Li6Chunhai Fan7Hao Pei8Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal UniversityShanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal UniversityShanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal UniversityShanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal UniversityShanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal UniversityDepartment of Gastroenterology and Hepatology, Zhongshan Hospital of Fudan UniversityShanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal UniversitySchool of Chemistry and Chemical Engineering, and Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityShanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal UniversityMHC-peptide multimers are important reagents for detecting antigen specific T cells. Here the authors show that DNA scaffolds can be used to make MHC-peptide multimers and the avidity controlled so that low abundance or T cells with low affinity TCR can be detected using these reagents.https://doi.org/10.1038/s41467-022-31684-8 |
| spellingShingle | Yueyang Sun Lu Yan Jiajia Sun Mingshu Xiao Wei Lai Guangqi Song Li Li Chunhai Fan Hao Pei Nanoscale organization of two-dimensional multimeric pMHC reagents with DNA origami for CD8+ T cell detection Nature Communications |
| title | Nanoscale organization of two-dimensional multimeric pMHC reagents with DNA origami for CD8+ T cell detection |
| title_full | Nanoscale organization of two-dimensional multimeric pMHC reagents with DNA origami for CD8+ T cell detection |
| title_fullStr | Nanoscale organization of two-dimensional multimeric pMHC reagents with DNA origami for CD8+ T cell detection |
| title_full_unstemmed | Nanoscale organization of two-dimensional multimeric pMHC reagents with DNA origami for CD8+ T cell detection |
| title_short | Nanoscale organization of two-dimensional multimeric pMHC reagents with DNA origami for CD8+ T cell detection |
| title_sort | nanoscale organization of two dimensional multimeric pmhc reagents with dna origami for cd8 t cell detection |
| url | https://doi.org/10.1038/s41467-022-31684-8 |
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