Characterization of the phospholipid and fatty acid composition of Sendai virus.

The lipid composition of Sendai virus, propagated in chicken eggs, was analyzed by high performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and gas-liquid chromatography (GLC). Phosphatidylcholine was found to be the dominant phospholipid (37.3%) with phosphatidylethanolamine...

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Main Authors: J A Barnes, D J Pehowich, T M Allen
Format: Article
Language:English
Published: Elsevier 1987-02-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520387149
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author J A Barnes
D J Pehowich
T M Allen
author_facet J A Barnes
D J Pehowich
T M Allen
author_sort J A Barnes
collection DOAJ
description The lipid composition of Sendai virus, propagated in chicken eggs, was analyzed by high performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and gas-liquid chromatography (GLC). Phosphatidylcholine was found to be the dominant phospholipid (37.3%) with phosphatidylethanolamine (26.8%) and phosphatidylserine (12.0%) also present in significant amounts. Analysis of the fatty acid methyl esters revealed that the dominant fatty acids in total phospholipid were: C16:0 (17.6%), C18:0 (15.4%), C18:1 (n-9) (22.0%), and C24:0 (6.0%). Cardiolipin, phosphatidylserine, and sphingomyelin contained higher levels of saturated fatty acids relative to phosphatidylinositol, phosphatidylethanolamine, and phosphatidylcholine.
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spelling doaj.art-318a2b2288d649b09e5bedd8a866d18e2022-12-21T21:58:18ZengElsevierJournal of Lipid Research0022-22751987-02-01282130137Characterization of the phospholipid and fatty acid composition of Sendai virus.J A BarnesD J PehowichT M AllenThe lipid composition of Sendai virus, propagated in chicken eggs, was analyzed by high performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and gas-liquid chromatography (GLC). Phosphatidylcholine was found to be the dominant phospholipid (37.3%) with phosphatidylethanolamine (26.8%) and phosphatidylserine (12.0%) also present in significant amounts. Analysis of the fatty acid methyl esters revealed that the dominant fatty acids in total phospholipid were: C16:0 (17.6%), C18:0 (15.4%), C18:1 (n-9) (22.0%), and C24:0 (6.0%). Cardiolipin, phosphatidylserine, and sphingomyelin contained higher levels of saturated fatty acids relative to phosphatidylinositol, phosphatidylethanolamine, and phosphatidylcholine.http://www.sciencedirect.com/science/article/pii/S0022227520387149
spellingShingle J A Barnes
D J Pehowich
T M Allen
Characterization of the phospholipid and fatty acid composition of Sendai virus.
Journal of Lipid Research
title Characterization of the phospholipid and fatty acid composition of Sendai virus.
title_full Characterization of the phospholipid and fatty acid composition of Sendai virus.
title_fullStr Characterization of the phospholipid and fatty acid composition of Sendai virus.
title_full_unstemmed Characterization of the phospholipid and fatty acid composition of Sendai virus.
title_short Characterization of the phospholipid and fatty acid composition of Sendai virus.
title_sort characterization of the phospholipid and fatty acid composition of sendai virus
url http://www.sciencedirect.com/science/article/pii/S0022227520387149
work_keys_str_mv AT jabarnes characterizationofthephospholipidandfattyacidcompositionofsendaivirus
AT djpehowich characterizationofthephospholipidandfattyacidcompositionofsendaivirus
AT tmallen characterizationofthephospholipidandfattyacidcompositionofsendaivirus