Characterization of the phospholipid and fatty acid composition of Sendai virus.
The lipid composition of Sendai virus, propagated in chicken eggs, was analyzed by high performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and gas-liquid chromatography (GLC). Phosphatidylcholine was found to be the dominant phospholipid (37.3%) with phosphatidylethanolamine...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
1987-02-01
|
Series: | Journal of Lipid Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520387149 |
_version_ | 1818672242642386944 |
---|---|
author | J A Barnes D J Pehowich T M Allen |
author_facet | J A Barnes D J Pehowich T M Allen |
author_sort | J A Barnes |
collection | DOAJ |
description | The lipid composition of Sendai virus, propagated in chicken eggs, was analyzed by high performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and gas-liquid chromatography (GLC). Phosphatidylcholine was found to be the dominant phospholipid (37.3%) with phosphatidylethanolamine (26.8%) and phosphatidylserine (12.0%) also present in significant amounts. Analysis of the fatty acid methyl esters revealed that the dominant fatty acids in total phospholipid were: C16:0 (17.6%), C18:0 (15.4%), C18:1 (n-9) (22.0%), and C24:0 (6.0%). Cardiolipin, phosphatidylserine, and sphingomyelin contained higher levels of saturated fatty acids relative to phosphatidylinositol, phosphatidylethanolamine, and phosphatidylcholine. |
first_indexed | 2024-12-17T07:36:47Z |
format | Article |
id | doaj.art-318a2b2288d649b09e5bedd8a866d18e |
institution | Directory Open Access Journal |
issn | 0022-2275 |
language | English |
last_indexed | 2024-12-17T07:36:47Z |
publishDate | 1987-02-01 |
publisher | Elsevier |
record_format | Article |
series | Journal of Lipid Research |
spelling | doaj.art-318a2b2288d649b09e5bedd8a866d18e2022-12-21T21:58:18ZengElsevierJournal of Lipid Research0022-22751987-02-01282130137Characterization of the phospholipid and fatty acid composition of Sendai virus.J A BarnesD J PehowichT M AllenThe lipid composition of Sendai virus, propagated in chicken eggs, was analyzed by high performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and gas-liquid chromatography (GLC). Phosphatidylcholine was found to be the dominant phospholipid (37.3%) with phosphatidylethanolamine (26.8%) and phosphatidylserine (12.0%) also present in significant amounts. Analysis of the fatty acid methyl esters revealed that the dominant fatty acids in total phospholipid were: C16:0 (17.6%), C18:0 (15.4%), C18:1 (n-9) (22.0%), and C24:0 (6.0%). Cardiolipin, phosphatidylserine, and sphingomyelin contained higher levels of saturated fatty acids relative to phosphatidylinositol, phosphatidylethanolamine, and phosphatidylcholine.http://www.sciencedirect.com/science/article/pii/S0022227520387149 |
spellingShingle | J A Barnes D J Pehowich T M Allen Characterization of the phospholipid and fatty acid composition of Sendai virus. Journal of Lipid Research |
title | Characterization of the phospholipid and fatty acid composition of Sendai virus. |
title_full | Characterization of the phospholipid and fatty acid composition of Sendai virus. |
title_fullStr | Characterization of the phospholipid and fatty acid composition of Sendai virus. |
title_full_unstemmed | Characterization of the phospholipid and fatty acid composition of Sendai virus. |
title_short | Characterization of the phospholipid and fatty acid composition of Sendai virus. |
title_sort | characterization of the phospholipid and fatty acid composition of sendai virus |
url | http://www.sciencedirect.com/science/article/pii/S0022227520387149 |
work_keys_str_mv | AT jabarnes characterizationofthephospholipidandfattyacidcompositionofsendaivirus AT djpehowich characterizationofthephospholipidandfattyacidcompositionofsendaivirus AT tmallen characterizationofthephospholipidandfattyacidcompositionofsendaivirus |