Exploiting oxidative phosphorylation to promote the stem and immunoevasive properties of pancreatic cancer stem cells
Long-term cultures of pancreatic cancer stem cells (PaCSCs) are difficult to obtain. Here, the authors present a 2D culture method, based on the use of galactose, to establish cell cultures from pancreatic ductal adenocarcinoma xenotransplants enriched in PaCSCs.
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-18954-z |
| _version_ | 1818890245701107712 |
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| author | Sandra Valle Sonia Alcalá Laura Martin-Hijano Pablo Cabezas-Sáinz Diego Navarro Edurne Ramos Muñoz Lourdes Yuste Kanishka Tiwary Karolin Walter Laura Ruiz-Cañas Marta Alonso-Nocelo Juan A. Rubiolo Emilio González-Arnay Christopher Heeschen Laura Garcia-Bermejo Patrick C. Hermann Laura Sánchez Patricia Sancho Miguel Ángel Fernández-Moreno Bruno Sainz |
| author_facet | Sandra Valle Sonia Alcalá Laura Martin-Hijano Pablo Cabezas-Sáinz Diego Navarro Edurne Ramos Muñoz Lourdes Yuste Kanishka Tiwary Karolin Walter Laura Ruiz-Cañas Marta Alonso-Nocelo Juan A. Rubiolo Emilio González-Arnay Christopher Heeschen Laura Garcia-Bermejo Patrick C. Hermann Laura Sánchez Patricia Sancho Miguel Ángel Fernández-Moreno Bruno Sainz |
| author_sort | Sandra Valle |
| collection | DOAJ |
| description | Long-term cultures of pancreatic cancer stem cells (PaCSCs) are difficult to obtain. Here, the authors present a 2D culture method, based on the use of galactose, to establish cell cultures from pancreatic ductal adenocarcinoma xenotransplants enriched in PaCSCs. |
| first_indexed | 2024-12-19T17:21:51Z |
| format | Article |
| id | doaj.art-318cab6a23144c398f4c1aba8ef19747 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T17:21:51Z |
| publishDate | 2020-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-318cab6a23144c398f4c1aba8ef197472022-12-21T20:12:39ZengNature PortfolioNature Communications2041-17232020-10-0111111910.1038/s41467-020-18954-zExploiting oxidative phosphorylation to promote the stem and immunoevasive properties of pancreatic cancer stem cellsSandra Valle0Sonia Alcalá1Laura Martin-Hijano2Pablo Cabezas-Sáinz3Diego Navarro4Edurne Ramos Muñoz5Lourdes Yuste6Kanishka Tiwary7Karolin Walter8Laura Ruiz-Cañas9Marta Alonso-Nocelo10Juan A. Rubiolo11Emilio González-Arnay12Christopher Heeschen13Laura Garcia-Bermejo14Patrick C. Hermann15Laura Sánchez16Patricia Sancho17Miguel Ángel Fernández-Moreno18Bruno Sainz19Department of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAMDepartment of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAMDepartment of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAMDepartment of Zoology, Genetics and Physical Anthropology, Veterinary Faculty, Universidad de Santiago de CompostelaDepartment of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAMBiomarkers and Therapeutic Targets Group - IRYCISDepartment of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAMDepartment of Internal Medicine I, Ulm UniversityDepartment of Internal Medicine I, Ulm UniversityDepartment of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAMDepartment of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAMDepartment of Zoology, Genetics and Physical Anthropology, Veterinary Faculty, Universidad de Santiago de CompostelaDepartment of Neurology, Universidad Autónoma de Madrid (UAM)Stem Cells & Cancer Group, Molecular Pathology Programme, Spanish National Cancer Research Centre (CNIO)Biomarkers and Therapeutic Targets Group - IRYCISDepartment of Internal Medicine I, Ulm UniversityDepartment of Zoology, Genetics and Physical Anthropology, Veterinary Faculty, Universidad de Santiago de CompostelaTranslational Research Unit, IIS AragónDepartment of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAMDepartment of Biochemistry, Universidad Autónoma de Madrid (UAM) and Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), CSIC-UAMLong-term cultures of pancreatic cancer stem cells (PaCSCs) are difficult to obtain. Here, the authors present a 2D culture method, based on the use of galactose, to establish cell cultures from pancreatic ductal adenocarcinoma xenotransplants enriched in PaCSCs.https://doi.org/10.1038/s41467-020-18954-z |
| spellingShingle | Sandra Valle Sonia Alcalá Laura Martin-Hijano Pablo Cabezas-Sáinz Diego Navarro Edurne Ramos Muñoz Lourdes Yuste Kanishka Tiwary Karolin Walter Laura Ruiz-Cañas Marta Alonso-Nocelo Juan A. Rubiolo Emilio González-Arnay Christopher Heeschen Laura Garcia-Bermejo Patrick C. Hermann Laura Sánchez Patricia Sancho Miguel Ángel Fernández-Moreno Bruno Sainz Exploiting oxidative phosphorylation to promote the stem and immunoevasive properties of pancreatic cancer stem cells Nature Communications |
| title | Exploiting oxidative phosphorylation to promote the stem and immunoevasive properties of pancreatic cancer stem cells |
| title_full | Exploiting oxidative phosphorylation to promote the stem and immunoevasive properties of pancreatic cancer stem cells |
| title_fullStr | Exploiting oxidative phosphorylation to promote the stem and immunoevasive properties of pancreatic cancer stem cells |
| title_full_unstemmed | Exploiting oxidative phosphorylation to promote the stem and immunoevasive properties of pancreatic cancer stem cells |
| title_short | Exploiting oxidative phosphorylation to promote the stem and immunoevasive properties of pancreatic cancer stem cells |
| title_sort | exploiting oxidative phosphorylation to promote the stem and immunoevasive properties of pancreatic cancer stem cells |
| url | https://doi.org/10.1038/s41467-020-18954-z |
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