Potassium Channels Kv1.3 and Kir2.1 But Not Kv1.5 Contribute to BV2 Cell Line and Primary Microglial Migration
(1) Background: As membrane channels contribute to different cell functions, understanding the underlying mechanisms becomes extremely important. A large number of neuronal channels have been investigated, however, less studied are the channels expressed in the glia population, particularly in micro...
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/4/2081 |
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| author | Ruxandra Anton Mihail Ghenghea Violeta Ristoiu Christophe Gattlen Marc-Rene Suter Petre Alexandru Cojocaru Aurel Popa-Wagner Bogdan Catalin Alexandru-Florian Deftu |
| author_facet | Ruxandra Anton Mihail Ghenghea Violeta Ristoiu Christophe Gattlen Marc-Rene Suter Petre Alexandru Cojocaru Aurel Popa-Wagner Bogdan Catalin Alexandru-Florian Deftu |
| author_sort | Ruxandra Anton |
| collection | DOAJ |
| description | (1) Background: As membrane channels contribute to different cell functions, understanding the underlying mechanisms becomes extremely important. A large number of neuronal channels have been investigated, however, less studied are the channels expressed in the glia population, particularly in microglia. In the present study, we focused on the function of the Kv1.3, Kv1.5 and Kir2.1 potassium channels expressed in both BV2 cells and primary microglia cultures, which may impact the cellular migration process. (2) Methods: Using an immunocytochemical approach, we were able to show the presence of the investigated channels in BV2 microglial cells, record their currents using a patch clamp and their role in cell migration using the scratch assay. The migration of the primary microglial cells in culture was assessed using cell culture inserts. (3) Results: By blocking each potassium channel, we showed that Kv1.3 and Kir2.1 but not Kv1.5 are essential for BV2 cell migration. Further, primary microglial cultures were obtained from a line of transgenic CX3CR1-eGFP mice that express fluorescent labeled microglia. The mice were subjected to a spared nerve injury model of pain and we found that microglia motility in an 8 µm insert was reduced 2 days after spared nerve injury (SNI) compared with sham conditions. Additional investigations showed a further impact on cell motility by specifically blocking Kv1.3 and Kir2.1 but not Kv1.5; (4) Conclusions: Our study highlights the importance of the Kv1.3 and Kir2.1 but not Kv1.5 potassium channels on microglia migration both in BV2 and primary cell cultures. |
| first_indexed | 2024-03-09T00:42:42Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T00:42:42Z |
| publishDate | 2021-02-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-318e99a5098b464f9472af048b5e17b32023-12-11T17:42:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01224208110.3390/ijms22042081Potassium Channels Kv1.3 and Kir2.1 But Not Kv1.5 Contribute to BV2 Cell Line and Primary Microglial MigrationRuxandra Anton0Mihail Ghenghea1Violeta Ristoiu2Christophe Gattlen3Marc-Rene Suter4Petre Alexandru Cojocaru5Aurel Popa-Wagner6Bogdan Catalin7Alexandru-Florian Deftu8Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest, 050095 București, RomaniaDepartment of Anatomy, Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest, 050095 București, RomaniaDepartment of Anatomy, Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest, 050095 București, RomaniaPain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and Faculty of Biology and Medicine (FBM), University of Lausanne (UNIL), 1011 Lausanne, SwitzerlandPain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and Faculty of Biology and Medicine (FBM), University of Lausanne (UNIL), 1011 Lausanne, SwitzerlandDepartment of Physiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaExperimental Research Center for Normal and Pathological Aging, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Physiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Anatomy, Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest, 050095 București, Romania(1) Background: As membrane channels contribute to different cell functions, understanding the underlying mechanisms becomes extremely important. A large number of neuronal channels have been investigated, however, less studied are the channels expressed in the glia population, particularly in microglia. In the present study, we focused on the function of the Kv1.3, Kv1.5 and Kir2.1 potassium channels expressed in both BV2 cells and primary microglia cultures, which may impact the cellular migration process. (2) Methods: Using an immunocytochemical approach, we were able to show the presence of the investigated channels in BV2 microglial cells, record their currents using a patch clamp and their role in cell migration using the scratch assay. The migration of the primary microglial cells in culture was assessed using cell culture inserts. (3) Results: By blocking each potassium channel, we showed that Kv1.3 and Kir2.1 but not Kv1.5 are essential for BV2 cell migration. Further, primary microglial cultures were obtained from a line of transgenic CX3CR1-eGFP mice that express fluorescent labeled microglia. The mice were subjected to a spared nerve injury model of pain and we found that microglia motility in an 8 µm insert was reduced 2 days after spared nerve injury (SNI) compared with sham conditions. Additional investigations showed a further impact on cell motility by specifically blocking Kv1.3 and Kir2.1 but not Kv1.5; (4) Conclusions: Our study highlights the importance of the Kv1.3 and Kir2.1 but not Kv1.5 potassium channels on microglia migration both in BV2 and primary cell cultures.https://www.mdpi.com/1422-0067/22/4/2081microglial cellspotassium channelsmigrationspared nerve injurypain |
| spellingShingle | Ruxandra Anton Mihail Ghenghea Violeta Ristoiu Christophe Gattlen Marc-Rene Suter Petre Alexandru Cojocaru Aurel Popa-Wagner Bogdan Catalin Alexandru-Florian Deftu Potassium Channels Kv1.3 and Kir2.1 But Not Kv1.5 Contribute to BV2 Cell Line and Primary Microglial Migration International Journal of Molecular Sciences microglial cells potassium channels migration spared nerve injury pain |
| title | Potassium Channels Kv1.3 and Kir2.1 But Not Kv1.5 Contribute to BV2 Cell Line and Primary Microglial Migration |
| title_full | Potassium Channels Kv1.3 and Kir2.1 But Not Kv1.5 Contribute to BV2 Cell Line and Primary Microglial Migration |
| title_fullStr | Potassium Channels Kv1.3 and Kir2.1 But Not Kv1.5 Contribute to BV2 Cell Line and Primary Microglial Migration |
| title_full_unstemmed | Potassium Channels Kv1.3 and Kir2.1 But Not Kv1.5 Contribute to BV2 Cell Line and Primary Microglial Migration |
| title_short | Potassium Channels Kv1.3 and Kir2.1 But Not Kv1.5 Contribute to BV2 Cell Line and Primary Microglial Migration |
| title_sort | potassium channels kv1 3 and kir2 1 but not kv1 5 contribute to bv2 cell line and primary microglial migration |
| topic | microglial cells potassium channels migration spared nerve injury pain |
| url | https://www.mdpi.com/1422-0067/22/4/2081 |
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