Effects of silver nanoparticle on lactate dehydrogenase activity and histological changes of heart tissue in male wistar rats
Background & Objective: The silver nanoparticles are important in many applications of nanoparticles on human health . The toxicity of silver nanoparticles are not well documented yet. The aim of this study was to investigate the effect of silver nanoparticles on lactate dehydrogenase activity a...
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| Format: | Article |
| Language: | fas |
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Fasa University of Medical Sciences
2013-03-01
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| Series: | Journal of Fasa University of Medical Sciences |
| Subjects: | |
| Online Access: | http://journal.fums.ac.ir/browse.php?a_code=A-10-26-29&slc_lang=en&sid=1 |
| _version_ | 1831598497355268096 |
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| author | Noushin Naghsh Amir Masoud Mashayekh Samaneh Khodadadi |
| author_facet | Noushin Naghsh Amir Masoud Mashayekh Samaneh Khodadadi |
| author_sort | Noushin Naghsh |
| collection | DOAJ |
| description | Background & Objective: The silver nanoparticles are important in many applications of nanoparticles on human health . The toxicity of silver nanoparticles are not well documented yet. The aim of this study was to investigate the effect of silver nanoparticles on lactate dehydrogenase activity and histological changes in heart tissue. Materials &Methods: In this study, 40 adult male wistar rats of 220±20gr were divided in to five groups including control and four experimental groups. The latter groups were injected intraperitoneally spherical nano silver particles of 50, 100, 200 and 400 ppm respectively for five consecutive days. Then three, eight and twelve days after the last injection, blood samples were collected and lactate dehydrogenase (LDH) activity was assayed . Also, tissue samples from the heart muscle were prepared and studied after staining with Hematoxiline-Eosine. Data of LDH activity was analyzed by One way- ANOVA- test and P-value of ≤ 0.05 were considered as significant. Results : The result showed that different concentrations of silver nanoparticles have no significant effect on the lactate dehydrogenase (p=0.192) . T he histological study of the tissue after exposure to 400 ppm concentration of silver nanoparticles showed the start of primary apoptosis in heart tissue. Conclusion: The LDH activity was not changed significantly after exposure to different concentration of silver nanoparticles, which shows the safety of these particles on LDH activity. |
| first_indexed | 2024-12-18T14:06:00Z |
| format | Article |
| id | doaj.art-3190e002affe4001b731ff2904654807 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2228-7329 |
| language | fas |
| last_indexed | 2024-12-18T14:06:00Z |
| publishDate | 2013-03-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Fasa University of Medical Sciences |
| spelling | doaj.art-3190e002affe4001b731ff29046548072022-12-21T21:05:14ZfasFasa University of Medical SciencesJournal of Fasa University of Medical Sciences2228-51052228-73292013-03-0124303307Effects of silver nanoparticle on lactate dehydrogenase activity and histological changes of heart tissue in male wistar ratsNoushin NaghshAmir Masoud MashayekhSamaneh KhodadadiBackground & Objective: The silver nanoparticles are important in many applications of nanoparticles on human health . The toxicity of silver nanoparticles are not well documented yet. The aim of this study was to investigate the effect of silver nanoparticles on lactate dehydrogenase activity and histological changes in heart tissue. Materials &Methods: In this study, 40 adult male wistar rats of 220±20gr were divided in to five groups including control and four experimental groups. The latter groups were injected intraperitoneally spherical nano silver particles of 50, 100, 200 and 400 ppm respectively for five consecutive days. Then three, eight and twelve days after the last injection, blood samples were collected and lactate dehydrogenase (LDH) activity was assayed . Also, tissue samples from the heart muscle were prepared and studied after staining with Hematoxiline-Eosine. Data of LDH activity was analyzed by One way- ANOVA- test and P-value of ≤ 0.05 were considered as significant. Results : The result showed that different concentrations of silver nanoparticles have no significant effect on the lactate dehydrogenase (p=0.192) . T he histological study of the tissue after exposure to 400 ppm concentration of silver nanoparticles showed the start of primary apoptosis in heart tissue. Conclusion: The LDH activity was not changed significantly after exposure to different concentration of silver nanoparticles, which shows the safety of these particles on LDH activity.http://journal.fums.ac.ir/browse.php?a_code=A-10-26-29&slc_lang=en&sid=1silver nanoparticleslactateDehydroganasetoxicityheart tissue |
| spellingShingle | Noushin Naghsh Amir Masoud Mashayekh Samaneh Khodadadi Effects of silver nanoparticle on lactate dehydrogenase activity and histological changes of heart tissue in male wistar rats Journal of Fasa University of Medical Sciences silver nanoparticles lactateDehydroganase toxicity heart tissue |
| title | Effects of silver nanoparticle on lactate dehydrogenase activity and histological changes of heart tissue in male wistar rats |
| title_full | Effects of silver nanoparticle on lactate dehydrogenase activity and histological changes of heart tissue in male wistar rats |
| title_fullStr | Effects of silver nanoparticle on lactate dehydrogenase activity and histological changes of heart tissue in male wistar rats |
| title_full_unstemmed | Effects of silver nanoparticle on lactate dehydrogenase activity and histological changes of heart tissue in male wistar rats |
| title_short | Effects of silver nanoparticle on lactate dehydrogenase activity and histological changes of heart tissue in male wistar rats |
| title_sort | effects of silver nanoparticle on lactate dehydrogenase activity and histological changes of heart tissue in male wistar rats |
| topic | silver nanoparticles lactateDehydroganase toxicity heart tissue |
| url | http://journal.fums.ac.ir/browse.php?a_code=A-10-26-29&slc_lang=en&sid=1 |
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