Certain Tomato Root Exudates Induced by <i>Pseudomonas stutzeri</i> NRCB010 Enhance Its Rhizosphere Colonization Capability
Plant growth-promoting rhizobacteria (PGPR) can colonize plant root surfaces or form biofilms to promote plant growth and enhance plant resistance to harsh external environments. However, plant–PGPR interactions, especially chemical signaling molecules, are poorly understood. This study aimed to gai...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/5/664 |
| _version_ | 1797599113569632256 |
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| author | Huanhuan Zhang Donghui Zheng Chun Hu Wenwen Cheng Peng Lei Hong Xu Nan Gao |
| author_facet | Huanhuan Zhang Donghui Zheng Chun Hu Wenwen Cheng Peng Lei Hong Xu Nan Gao |
| author_sort | Huanhuan Zhang |
| collection | DOAJ |
| description | Plant growth-promoting rhizobacteria (PGPR) can colonize plant root surfaces or form biofilms to promote plant growth and enhance plant resistance to harsh external environments. However, plant–PGPR interactions, especially chemical signaling molecules, are poorly understood. This study aimed to gain an in-depth understanding of the rhizosphere interaction mechanisms between PGPR and tomato plants. This study found that inoculation with a certain concentration of <i>Pseudomonas stutzeri</i> significantly promoted tomato growth and induced significant changes in tomato root exudates. Furthermore, the root exudates significantly induced NRCB010 growth, swarming motility, and biofilm formation. In addition, the composition of the root exudates was analyzed, and four metabolites (methyl hexadecanoate, methyl stearate, 2,4-di-tert-butylphenol, and n-hexadecanoic acid) significantly related to the chemotaxis and biofilm formation of NRCB010 were screened. Further assessment showed that these metabolites positively affected the growth, swarming motility, chemotaxis, or biofilm formation of strain NRCB010. Among these, n-hexadecanoic acid induced the most remarkable growth, chemotactic response, biofilm formation, and rhizosphere colonization. This study will help develop effective PGPR-based bioformulations to improve PGPR colonization and crop yields. |
| first_indexed | 2024-03-11T03:30:08Z |
| format | Article |
| id | doaj.art-319222be217a46fda4e824c825e2eab9 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T03:30:08Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-319222be217a46fda4e824c825e2eab92023-11-18T02:26:10ZengMDPI AGMetabolites2218-19892023-05-0113566410.3390/metabo13050664Certain Tomato Root Exudates Induced by <i>Pseudomonas stutzeri</i> NRCB010 Enhance Its Rhizosphere Colonization CapabilityHuanhuan Zhang0Donghui Zheng1Chun Hu2Wenwen Cheng3Peng Lei4Hong Xu5Nan Gao6School of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, ChinaCollege of 2011, Nanjing Tech University, Nanjing 211816, ChinaSchool of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, ChinaSchool of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, ChinaSchool of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, ChinaSchool of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, ChinaSchool of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, ChinaPlant growth-promoting rhizobacteria (PGPR) can colonize plant root surfaces or form biofilms to promote plant growth and enhance plant resistance to harsh external environments. However, plant–PGPR interactions, especially chemical signaling molecules, are poorly understood. This study aimed to gain an in-depth understanding of the rhizosphere interaction mechanisms between PGPR and tomato plants. This study found that inoculation with a certain concentration of <i>Pseudomonas stutzeri</i> significantly promoted tomato growth and induced significant changes in tomato root exudates. Furthermore, the root exudates significantly induced NRCB010 growth, swarming motility, and biofilm formation. In addition, the composition of the root exudates was analyzed, and four metabolites (methyl hexadecanoate, methyl stearate, 2,4-di-tert-butylphenol, and n-hexadecanoic acid) significantly related to the chemotaxis and biofilm formation of NRCB010 were screened. Further assessment showed that these metabolites positively affected the growth, swarming motility, chemotaxis, or biofilm formation of strain NRCB010. Among these, n-hexadecanoic acid induced the most remarkable growth, chemotactic response, biofilm formation, and rhizosphere colonization. This study will help develop effective PGPR-based bioformulations to improve PGPR colonization and crop yields.https://www.mdpi.com/2218-1989/13/5/664PGPR<i>Pseudomonas</i> sp.chemotaxisbiofilmcolonizationn-hexadecanoic acid |
| spellingShingle | Huanhuan Zhang Donghui Zheng Chun Hu Wenwen Cheng Peng Lei Hong Xu Nan Gao Certain Tomato Root Exudates Induced by <i>Pseudomonas stutzeri</i> NRCB010 Enhance Its Rhizosphere Colonization Capability Metabolites PGPR <i>Pseudomonas</i> sp. chemotaxis biofilm colonization n-hexadecanoic acid |
| title | Certain Tomato Root Exudates Induced by <i>Pseudomonas stutzeri</i> NRCB010 Enhance Its Rhizosphere Colonization Capability |
| title_full | Certain Tomato Root Exudates Induced by <i>Pseudomonas stutzeri</i> NRCB010 Enhance Its Rhizosphere Colonization Capability |
| title_fullStr | Certain Tomato Root Exudates Induced by <i>Pseudomonas stutzeri</i> NRCB010 Enhance Its Rhizosphere Colonization Capability |
| title_full_unstemmed | Certain Tomato Root Exudates Induced by <i>Pseudomonas stutzeri</i> NRCB010 Enhance Its Rhizosphere Colonization Capability |
| title_short | Certain Tomato Root Exudates Induced by <i>Pseudomonas stutzeri</i> NRCB010 Enhance Its Rhizosphere Colonization Capability |
| title_sort | certain tomato root exudates induced by i pseudomonas stutzeri i nrcb010 enhance its rhizosphere colonization capability |
| topic | PGPR <i>Pseudomonas</i> sp. chemotaxis biofilm colonization n-hexadecanoic acid |
| url | https://www.mdpi.com/2218-1989/13/5/664 |
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