Detection of Antimicrobial Resistance of Bacteria <i>Staphylococcus chromogenes</i> Isolated from Sheep’s Milk and Cheese
Antimicrobial and multidrug resistance is detected in nonaureus staphylococci, including <i>Staphylococcus chromogenes</i>, which commonly causes intramammary infections. Recent clinical studies point to the presence of methicillin-resistant <i>S. chromogenes.</i> Therefore,...
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MDPI AG
2021-05-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/5/570 |
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| author | Ivana Regecová Jana Výrostková František Zigo Gabriela Gregová Mariana Kováčová |
| author_facet | Ivana Regecová Jana Výrostková František Zigo Gabriela Gregová Mariana Kováčová |
| author_sort | Ivana Regecová |
| collection | DOAJ |
| description | Antimicrobial and multidrug resistance is detected in nonaureus staphylococci, including <i>Staphylococcus chromogenes</i>, which commonly causes intramammary infections. Recent clinical studies point to the presence of methicillin-resistant <i>S. chromogenes.</i> Therefore, this study aims to determine the prevalence of this species in samples of sheep‘s milk and cheeses made from them. Isolates were identified by polymerase chain reaction and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF). A total of 208 staphylococcal isolates were identified. Of these, 18% were identified as <i>S. chromogenes</i>. The antimicrobial resistance of the identified isolates was determined using the agar dilution method against penicillin, ceftaroline, teicoplanin, gentamicin, erythromycin, tetracycline, and ofloxacin. The highest resistance was found to penicillin (95%), tetracycline (86%), and oxacillin (81%). The highest sensitivity was confirmed for gentamicin (55%). The study also confirmed the presence of methicillin resistant staphylococcal isolates (30%) based on the phenotypic manifestation of antimicrobial resistance and detection of the presence of the <i>mecA</i> gene. The study shows that the tested isolates (62%) were multidrug resistant. Resistance to two antibiotics was most often found (39%). |
| first_indexed | 2024-03-10T11:29:31Z |
| format | Article |
| id | doaj.art-319273c7013045c7bc9cfc978b9eec43 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T11:29:31Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-319273c7013045c7bc9cfc978b9eec432023-11-21T19:21:53ZengMDPI AGAntibiotics2079-63822021-05-0110557010.3390/antibiotics10050570Detection of Antimicrobial Resistance of Bacteria <i>Staphylococcus chromogenes</i> Isolated from Sheep’s Milk and CheeseIvana Regecová0Jana Výrostková1František Zigo2Gabriela Gregová3Mariana Kováčová4Department of Food Hygiene, Technology and Safety, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, SlovakiaDepartment of Food Hygiene, Technology and Safety, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, SlovakiaDepartment of Animal Nutrition and Husbandry, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, SlovakiaDepartment of Public Veterinary Medicine and Animal Welfare, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, SlovakiaDepartment of Food Hygiene, Technology and Safety, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, SlovakiaAntimicrobial and multidrug resistance is detected in nonaureus staphylococci, including <i>Staphylococcus chromogenes</i>, which commonly causes intramammary infections. Recent clinical studies point to the presence of methicillin-resistant <i>S. chromogenes.</i> Therefore, this study aims to determine the prevalence of this species in samples of sheep‘s milk and cheeses made from them. Isolates were identified by polymerase chain reaction and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF). A total of 208 staphylococcal isolates were identified. Of these, 18% were identified as <i>S. chromogenes</i>. The antimicrobial resistance of the identified isolates was determined using the agar dilution method against penicillin, ceftaroline, teicoplanin, gentamicin, erythromycin, tetracycline, and ofloxacin. The highest resistance was found to penicillin (95%), tetracycline (86%), and oxacillin (81%). The highest sensitivity was confirmed for gentamicin (55%). The study also confirmed the presence of methicillin resistant staphylococcal isolates (30%) based on the phenotypic manifestation of antimicrobial resistance and detection of the presence of the <i>mecA</i> gene. The study shows that the tested isolates (62%) were multidrug resistant. Resistance to two antibiotics was most often found (39%).https://www.mdpi.com/2079-6382/10/5/570antibiotic<i>mecA</i> geneMALDI–TOFPCRstaphylococci |
| spellingShingle | Ivana Regecová Jana Výrostková František Zigo Gabriela Gregová Mariana Kováčová Detection of Antimicrobial Resistance of Bacteria <i>Staphylococcus chromogenes</i> Isolated from Sheep’s Milk and Cheese Antibiotics antibiotic <i>mecA</i> gene MALDI–TOF PCR staphylococci |
| title | Detection of Antimicrobial Resistance of Bacteria <i>Staphylococcus chromogenes</i> Isolated from Sheep’s Milk and Cheese |
| title_full | Detection of Antimicrobial Resistance of Bacteria <i>Staphylococcus chromogenes</i> Isolated from Sheep’s Milk and Cheese |
| title_fullStr | Detection of Antimicrobial Resistance of Bacteria <i>Staphylococcus chromogenes</i> Isolated from Sheep’s Milk and Cheese |
| title_full_unstemmed | Detection of Antimicrobial Resistance of Bacteria <i>Staphylococcus chromogenes</i> Isolated from Sheep’s Milk and Cheese |
| title_short | Detection of Antimicrobial Resistance of Bacteria <i>Staphylococcus chromogenes</i> Isolated from Sheep’s Milk and Cheese |
| title_sort | detection of antimicrobial resistance of bacteria i staphylococcus chromogenes i isolated from sheep s milk and cheese |
| topic | antibiotic <i>mecA</i> gene MALDI–TOF PCR staphylococci |
| url | https://www.mdpi.com/2079-6382/10/5/570 |
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