An Earliest Endosymbiont, <i>Wolbachia massiliensis</i> sp. nov., Strain PL13 from the Bed Bug (<i>Cimex hemipterus</i>), Type Strain of a New Supergroup T
The symbiotic <i>Wolbachia</i> are the most sophisticated mutualistic bacterium among all insect-associated microbiota. <i>Wolbachia</i>-insect relationship fluctuates from the simple facultative/parasitic to an obligate nutritional-mutualistic association as it was the case...
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MDPI AG
2020-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/21/8064 |
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| author | Younes Laidoudi Anthony Levasseur Hacène Medkour Mossaab Maaloum Mariem Ben Khedher Masse Sambou Hubert Bassene Bernard Davoust Florence Fenollar Didier Raoult Oleg Mediannikov |
| author_facet | Younes Laidoudi Anthony Levasseur Hacène Medkour Mossaab Maaloum Mariem Ben Khedher Masse Sambou Hubert Bassene Bernard Davoust Florence Fenollar Didier Raoult Oleg Mediannikov |
| author_sort | Younes Laidoudi |
| collection | DOAJ |
| description | The symbiotic <i>Wolbachia</i> are the most sophisticated mutualistic bacterium among all insect-associated microbiota. <i>Wolbachia</i>-insect relationship fluctuates from the simple facultative/parasitic to an obligate nutritional-mutualistic association as it was the case of the bedbug-<i>Wolbachia</i> from <i>Cimex</i><i>lectularius</i>. Understanding this association may help in the control of associated arthropods. Genomic data have proven to be reliable tools in resolving some aspects of these symbiotic associations. Although, <i>Wolbachia</i> appear to be fastidious or uncultivated bacteria which strongly limited their study. Here we proposed <i>Drosophila</i> S2 cell line for the isolation and culture model to study <i>Wolbachia</i> strains. We therefore isolated and characterized a novel <i>Wolbachia</i> strain associated with the bedbug <i>Cimex</i><i>hemipterus</i>, designated as <i>wChem</i> strain PL13, and proposed <i>Wolbachia</i><i>massiliensis</i> sp. nov. strain <i>wChem</i>-PL13 a type strain of this new species from new supergroup T. Phylogenetically, T-supergroup was close to F and S-supergroups from insects and D-supergroup from filarial nematodes. We determined the 1,291,339-bp genome of <i>wChem</i>-PL13, which was the smallest insect-associated <i>Wolbachia</i> genomes. Overall, the <i>wChem</i> genome shared 50% of protein coding genes with the other insect-associated facultative <i>Wolbachia</i> strains. These findings highlight the diversity of <i>Wolbachia</i> genotypes as well as the <i>Wolbachia</i>-host relationship among Cimicinae subfamily. The <i>wChem</i> provides folate and riboflavin vitamins on which the host depends, while the bacteria had a limited translation mechanism suggesting its strong dependence to its hosts. However, the clear-cut distinction between mutualism and parasitism of the <i>wChem</i> in <i>C</i>. <i>hemipterus</i> cannot be yet ruled out. |
| first_indexed | 2024-03-10T15:14:39Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T15:14:39Z |
| publishDate | 2020-10-01 |
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| spelling | doaj.art-3196d7f8a25f4e6fac4ab4c4b9be14da2023-11-20T19:00:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-012121806410.3390/ijms21218064An Earliest Endosymbiont, <i>Wolbachia massiliensis</i> sp. nov., Strain PL13 from the Bed Bug (<i>Cimex hemipterus</i>), Type Strain of a New Supergroup TYounes Laidoudi0Anthony Levasseur1Hacène Medkour2Mossaab Maaloum3Mariem Ben Khedher4Masse Sambou5Hubert Bassene6Bernard Davoust7Florence Fenollar8Didier Raoult9Oleg Mediannikov10Aix Marseille Univ, IRD, AP-HM, MEPHI, 13385 Marseille, FranceAix Marseille Univ, IRD, AP-HM, MEPHI, 13385 Marseille, FranceAix Marseille Univ, IRD, AP-HM, MEPHI, 13385 Marseille, FranceLaboratory of Biology and Health, Faculty of Sciences Ben M’sik, Hassan II University, Sidi Othmane, Casablanca 7955, MoroccoAix Marseille Univ, IRD, AP-HM, MEPHI, 13385 Marseille, FranceAix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France, 13385 Marseille, FranceAix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France, 13385 Marseille, FranceAix Marseille Univ, IRD, AP-HM, MEPHI, 13385 Marseille, FranceIHU Méditerranée Infection, 13385 Marseille, FranceAix Marseille Univ, IRD, AP-HM, MEPHI, 13385 Marseille, FranceAix Marseille Univ, IRD, AP-HM, MEPHI, 13385 Marseille, FranceThe symbiotic <i>Wolbachia</i> are the most sophisticated mutualistic bacterium among all insect-associated microbiota. <i>Wolbachia</i>-insect relationship fluctuates from the simple facultative/parasitic to an obligate nutritional-mutualistic association as it was the case of the bedbug-<i>Wolbachia</i> from <i>Cimex</i><i>lectularius</i>. Understanding this association may help in the control of associated arthropods. Genomic data have proven to be reliable tools in resolving some aspects of these symbiotic associations. Although, <i>Wolbachia</i> appear to be fastidious or uncultivated bacteria which strongly limited their study. Here we proposed <i>Drosophila</i> S2 cell line for the isolation and culture model to study <i>Wolbachia</i> strains. We therefore isolated and characterized a novel <i>Wolbachia</i> strain associated with the bedbug <i>Cimex</i><i>hemipterus</i>, designated as <i>wChem</i> strain PL13, and proposed <i>Wolbachia</i><i>massiliensis</i> sp. nov. strain <i>wChem</i>-PL13 a type strain of this new species from new supergroup T. Phylogenetically, T-supergroup was close to F and S-supergroups from insects and D-supergroup from filarial nematodes. We determined the 1,291,339-bp genome of <i>wChem</i>-PL13, which was the smallest insect-associated <i>Wolbachia</i> genomes. Overall, the <i>wChem</i> genome shared 50% of protein coding genes with the other insect-associated facultative <i>Wolbachia</i> strains. These findings highlight the diversity of <i>Wolbachia</i> genotypes as well as the <i>Wolbachia</i>-host relationship among Cimicinae subfamily. The <i>wChem</i> provides folate and riboflavin vitamins on which the host depends, while the bacteria had a limited translation mechanism suggesting its strong dependence to its hosts. However, the clear-cut distinction between mutualism and parasitism of the <i>wChem</i> in <i>C</i>. <i>hemipterus</i> cannot be yet ruled out.https://www.mdpi.com/1422-0067/21/21/8064<i>Wolbachia</i>bedbug<i>Cimex hemipterus</i>isolationculturegenomics |
| spellingShingle | Younes Laidoudi Anthony Levasseur Hacène Medkour Mossaab Maaloum Mariem Ben Khedher Masse Sambou Hubert Bassene Bernard Davoust Florence Fenollar Didier Raoult Oleg Mediannikov An Earliest Endosymbiont, <i>Wolbachia massiliensis</i> sp. nov., Strain PL13 from the Bed Bug (<i>Cimex hemipterus</i>), Type Strain of a New Supergroup T International Journal of Molecular Sciences <i>Wolbachia</i> bedbug <i>Cimex hemipterus</i> isolation culture genomics |
| title | An Earliest Endosymbiont, <i>Wolbachia massiliensis</i> sp. nov., Strain PL13 from the Bed Bug (<i>Cimex hemipterus</i>), Type Strain of a New Supergroup T |
| title_full | An Earliest Endosymbiont, <i>Wolbachia massiliensis</i> sp. nov., Strain PL13 from the Bed Bug (<i>Cimex hemipterus</i>), Type Strain of a New Supergroup T |
| title_fullStr | An Earliest Endosymbiont, <i>Wolbachia massiliensis</i> sp. nov., Strain PL13 from the Bed Bug (<i>Cimex hemipterus</i>), Type Strain of a New Supergroup T |
| title_full_unstemmed | An Earliest Endosymbiont, <i>Wolbachia massiliensis</i> sp. nov., Strain PL13 from the Bed Bug (<i>Cimex hemipterus</i>), Type Strain of a New Supergroup T |
| title_short | An Earliest Endosymbiont, <i>Wolbachia massiliensis</i> sp. nov., Strain PL13 from the Bed Bug (<i>Cimex hemipterus</i>), Type Strain of a New Supergroup T |
| title_sort | earliest endosymbiont i wolbachia massiliensis i sp nov strain pl13 from the bed bug i cimex hemipterus i type strain of a new supergroup t |
| topic | <i>Wolbachia</i> bedbug <i>Cimex hemipterus</i> isolation culture genomics |
| url | https://www.mdpi.com/1422-0067/21/21/8064 |
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