In Vitro Antioxidant, Antitumor and Photocatalytic Activities of Silver Nanoparticles Synthesized Using <i>Equisetum</i> Species: A Green Approach
The ethanolic extracts of three <i>Equisetum</i> species (<i>E. pratense</i> Ehrh., <i>E. sylvaticum</i> L. and <i>E. telmateia</i> Ehrh.) were used to reduce silver ions to silver nanoparticles (AgNPs). The synthesized AgNPs were characterized using U...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/23/7325 |
| _version_ | 1797507339848253440 |
|---|---|
| author | Denisa Batir-Marin Cornelia Mircea Monica Boev Ana Flavia Burlec Andreia Corciova Adrian Fifere Alexandra Iacobescu Oana Cioanca Liliana Verestiuc Monica Hancianu |
| author_facet | Denisa Batir-Marin Cornelia Mircea Monica Boev Ana Flavia Burlec Andreia Corciova Adrian Fifere Alexandra Iacobescu Oana Cioanca Liliana Verestiuc Monica Hancianu |
| author_sort | Denisa Batir-Marin |
| collection | DOAJ |
| description | The ethanolic extracts of three <i>Equisetum</i> species (<i>E. pratense</i> Ehrh., <i>E. sylvaticum</i> L. and <i>E. telmateia</i> Ehrh.) were used to reduce silver ions to silver nanoparticles (AgNPs). The synthesized AgNPs were characterized using UV-Vis spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), Energy Dispersive X-ray (EDX), Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS) measurements. FTIR data revealed the functional groups of biomolecules involved in AgNPs synthesis, such as O-H, C-H, C=O, C-O, and C-C. EDX spectroscopy was used to highlight the presence of silver, while DLS spectroscopy provided information on the mean diameter of AgNPs, that ranged from 74.4 to 314 nm. The negative Zeta potential values (−23.76 for Ep–AgNPs, −29.54 for Es–AgNPs and −20.72 for Et–AgNPs) indicate the stability of the obtained colloidal solution. The study also focused on establishing the photocatalytic activity of AgNPs, which is an important aspect in terms of removing organic dyes from the environment. The best photocatalytic activity was observed for AgNPs obtained from <i>E. telmateia</i>, which degraded malachite green in a proportion of 97.9%. The antioxidant action of the three AgNPs samples was highlighted comparatively through four tests, with the best overall antioxidant capacity being observed for AgNPs obtained using <i>E. sylvaticum</i>. Moreover, the biosynthesized AgNPs showed promising cytotoxic efficacy against cancerous cell line MG63, the AgNPs obtained from <i>E. sylvaticum</i> L. providing the best result, with a LD<sub>50</sub> value around 1.5 mg/mL. |
| first_indexed | 2024-03-10T04:47:08Z |
| format | Article |
| id | doaj.art-31988855c1d542448c07e4f12998dd93 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T04:47:08Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-31988855c1d542448c07e4f12998dd932023-11-23T02:50:47ZengMDPI AGMolecules1420-30492021-12-012623732510.3390/molecules26237325In Vitro Antioxidant, Antitumor and Photocatalytic Activities of Silver Nanoparticles Synthesized Using <i>Equisetum</i> Species: A Green ApproachDenisa Batir-Marin0Cornelia Mircea1Monica Boev2Ana Flavia Burlec3Andreia Corciova4Adrian Fifere5Alexandra Iacobescu6Oana Cioanca7Liliana Verestiuc8Monica Hancianu9Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmacy, Dunarea de Jos University, 800010 Galati, RomaniaDepartment of Pharmaceutical Biochemistry and Clinical Laboratory, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, RomaniaDepartment of Pharmaceutical Sciences, Faculty of Medicine and Pharmacy, Dunarea de Jos University, 800010 Galati, RomaniaDepartment of Drug Analysis, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, RomaniaDepartment of Drug Analysis, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, RomaniaCentre of Advanced Research in Bionanoconjugates and Biopolymers Department, “Petru Poni” Institute of Macromolecular Chemistry, 41A Grigore Ghica Voda Alley, 700487 Iasi, RomaniaCentre of Advanced Research in Bionanoconjugates and Biopolymers Department, “Petru Poni” Institute of Macromolecular Chemistry, 41A Grigore Ghica Voda Alley, 700487 Iasi, RomaniaDepartment of Pharmacognosy, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, RomaniaDepartment of Biomedical Sciences, Faculty of Medical Bioengineering, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, RomaniaDepartment of Pharmacognosy, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, RomaniaThe ethanolic extracts of three <i>Equisetum</i> species (<i>E. pratense</i> Ehrh., <i>E. sylvaticum</i> L. and <i>E. telmateia</i> Ehrh.) were used to reduce silver ions to silver nanoparticles (AgNPs). The synthesized AgNPs were characterized using UV-Vis spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), Energy Dispersive X-ray (EDX), Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS) measurements. FTIR data revealed the functional groups of biomolecules involved in AgNPs synthesis, such as O-H, C-H, C=O, C-O, and C-C. EDX spectroscopy was used to highlight the presence of silver, while DLS spectroscopy provided information on the mean diameter of AgNPs, that ranged from 74.4 to 314 nm. The negative Zeta potential values (−23.76 for Ep–AgNPs, −29.54 for Es–AgNPs and −20.72 for Et–AgNPs) indicate the stability of the obtained colloidal solution. The study also focused on establishing the photocatalytic activity of AgNPs, which is an important aspect in terms of removing organic dyes from the environment. The best photocatalytic activity was observed for AgNPs obtained from <i>E. telmateia</i>, which degraded malachite green in a proportion of 97.9%. The antioxidant action of the three AgNPs samples was highlighted comparatively through four tests, with the best overall antioxidant capacity being observed for AgNPs obtained using <i>E. sylvaticum</i>. Moreover, the biosynthesized AgNPs showed promising cytotoxic efficacy against cancerous cell line MG63, the AgNPs obtained from <i>E. sylvaticum</i> L. providing the best result, with a LD<sub>50</sub> value around 1.5 mg/mL.https://www.mdpi.com/1420-3049/26/23/7325<i>Equisetum pratense</i><i>Equisetum telmateia</i><i>Equisetum sylvaticum</i>silver nanoparticlesgreen synthesisphotocatalytic activity |
| spellingShingle | Denisa Batir-Marin Cornelia Mircea Monica Boev Ana Flavia Burlec Andreia Corciova Adrian Fifere Alexandra Iacobescu Oana Cioanca Liliana Verestiuc Monica Hancianu In Vitro Antioxidant, Antitumor and Photocatalytic Activities of Silver Nanoparticles Synthesized Using <i>Equisetum</i> Species: A Green Approach Molecules <i>Equisetum pratense</i> <i>Equisetum telmateia</i> <i>Equisetum sylvaticum</i> silver nanoparticles green synthesis photocatalytic activity |
| title | In Vitro Antioxidant, Antitumor and Photocatalytic Activities of Silver Nanoparticles Synthesized Using <i>Equisetum</i> Species: A Green Approach |
| title_full | In Vitro Antioxidant, Antitumor and Photocatalytic Activities of Silver Nanoparticles Synthesized Using <i>Equisetum</i> Species: A Green Approach |
| title_fullStr | In Vitro Antioxidant, Antitumor and Photocatalytic Activities of Silver Nanoparticles Synthesized Using <i>Equisetum</i> Species: A Green Approach |
| title_full_unstemmed | In Vitro Antioxidant, Antitumor and Photocatalytic Activities of Silver Nanoparticles Synthesized Using <i>Equisetum</i> Species: A Green Approach |
| title_short | In Vitro Antioxidant, Antitumor and Photocatalytic Activities of Silver Nanoparticles Synthesized Using <i>Equisetum</i> Species: A Green Approach |
| title_sort | in vitro antioxidant antitumor and photocatalytic activities of silver nanoparticles synthesized using i equisetum i species a green approach |
| topic | <i>Equisetum pratense</i> <i>Equisetum telmateia</i> <i>Equisetum sylvaticum</i> silver nanoparticles green synthesis photocatalytic activity |
| url | https://www.mdpi.com/1420-3049/26/23/7325 |
| work_keys_str_mv | AT denisabatirmarin invitroantioxidantantitumorandphotocatalyticactivitiesofsilvernanoparticlessynthesizedusingiequisetumispeciesagreenapproach AT corneliamircea invitroantioxidantantitumorandphotocatalyticactivitiesofsilvernanoparticlessynthesizedusingiequisetumispeciesagreenapproach AT monicaboev invitroantioxidantantitumorandphotocatalyticactivitiesofsilvernanoparticlessynthesizedusingiequisetumispeciesagreenapproach AT anaflaviaburlec invitroantioxidantantitumorandphotocatalyticactivitiesofsilvernanoparticlessynthesizedusingiequisetumispeciesagreenapproach AT andreiacorciova invitroantioxidantantitumorandphotocatalyticactivitiesofsilvernanoparticlessynthesizedusingiequisetumispeciesagreenapproach AT adrianfifere invitroantioxidantantitumorandphotocatalyticactivitiesofsilvernanoparticlessynthesizedusingiequisetumispeciesagreenapproach AT alexandraiacobescu invitroantioxidantantitumorandphotocatalyticactivitiesofsilvernanoparticlessynthesizedusingiequisetumispeciesagreenapproach AT oanacioanca invitroantioxidantantitumorandphotocatalyticactivitiesofsilvernanoparticlessynthesizedusingiequisetumispeciesagreenapproach AT lilianaverestiuc invitroantioxidantantitumorandphotocatalyticactivitiesofsilvernanoparticlessynthesizedusingiequisetumispeciesagreenapproach AT monicahancianu invitroantioxidantantitumorandphotocatalyticactivitiesofsilvernanoparticlessynthesizedusingiequisetumispeciesagreenapproach |