Complementarity of Selective Culture and qPCR for Colistin Resistance Screening in Fresh and Frozen Pig Cecum Samples

Complementarity of Selective Culture and qPCR for Colistin Resistance Screening in Fresh and Frozen Pig Cecum Samples

Retrospective studies involving the screening of frozen stored collections of samples are commonplace when a new threat emerges, but it has been demonstrated that the freeze-thaw process can affect bacterial viability. The study of colistin-resistant bacteria in human and animal samples is an exampl...

Full description

Bibliographic Details
Main Authors:	Pedro Miguela-Villoldo, Miguel A. Moreno, Marta Hernández, David Rodríguez-Lázaro, Alejandro Gallardo, Carmen Borge, Alberto Quesada, Lucas Domínguez, María Ugarte-Ruiz
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2020-11-01
Series:	Frontiers in Microbiology
Subjects:	freeze-thaw process caecal samples mcr-1 mcr-2 swine antimicrobial resistance
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2020.572712/full

Similar Items

Involvement of <i>hpap2</i> and <i>dgkA</i> Genes in Colistin Resistance Mediated by <i>mcr</i> Determinants
by: Alejandro Gallardo, et al.
Published: (2020-08-01)

Colistin Selection of the <i>Mcr-1</i> Gene in Broiler Chicken Intestinal Microbiota
by: Pedro Miguela-Villoldo, et al.
Published: (2021-06-01)

Characterisation of mobile colistin resistance genes (mcr-3 and mcr-5) in river and storm water in regions of the Western Cape of South Africa
by: Yolandi Snyman, et al.
Published: (2021-06-01)

Carbapenemase-Producing Elizabethkingia Meningoseptica from Healthy Pigs Associated with Colistin Use in Spain
by: Pedro Miguela-Villoldo, et al.
Published: (2019-09-01)

Identification of novel mobile colistin resistance gene mcr-10
by: Chengcheng Wang, et al.
Published: (2020-01-01)

The first report of emerging mobilized colistin-resistance (mcr) genes and ERIC-PCR typing in Escherichia coli and Klebsiella pneumoniae clinical isolates in southwest Iran
by: Moosavian M, et al.
Published: (2019-04-01)

A Multiplex SYBR Green Real-Time PCR Assay for the Detection of Three Colistin Resistance Genes from Cultured Bacteria, Feces, and Environment Samples
by: Jiyun Li, et al.
Published: (2017-10-01)

Deciphering MCR-2 Colistin Resistance
by: Jian Sun, et al.
Published: (2017-07-01)

Molecular survey of mcr1 and mcr2 plasmid mediated colistin resistance genes in Escherichia coli isolates of animal origin in Iran
by: Kayhan Ilbeigi, et al.
Published: (2021-03-01)

Characterization of two novel colistin resistance gene mcr-1 variants originated from Moraxella spp.
by: Yongliang Che, et al.
Published: (2023-05-01)

Emergence of Colistin Resistance Gene mcr-8 and Its Variant in Raoultella ornithinolytica
by: Xiaoming Wang, et al.
Published: (2019-02-01)

Colistin Resistance and Molecular Characterization of the Genomes of mcr-1-Positive Escherichia coli Clinical Isolates
by: Qiaoling Li, et al.
Published: (2022-05-01)

Reply to Cabello et al., “Aquaculture and <italic toggle="yes">mcr</italic> Colistin Resistance Determinants”
by: Yingbo Shen, et al.
Published: (2018-09-01)

Very low prevalence of MCR-1/MCR-2 plasmid-mediated colistin resistance in urinary tract Enterobacteriaceae in Switzerland
by: Nadia Liassine, et al.
Published: (2016-10-01)

LBJMR medium: a new polyvalent culture medium for isolating and selecting vancomycin and colistin-resistant bacteria
by: Lucie Bardet, et al.
Published: (2017-11-01)

Identification of Novel Mobilized Colistin Resistance Gene <italic toggle="yes">mcr-9</italic> in a Multidrug-Resistant, Colistin-Susceptible <named-content content-type="genus-species">Salmonella enterica</named-content> Serotype Typhimurium Isolate
by: Laura M. Carroll, et al.
Published: (2019-06-01)

Prevalence and molecular epidemiology of mcr-mediated colistin-resistance Escherichia coli from healthy poultry in France after national plan to reduce exposure to colistin in farm
by: Agnès Perrin-Guyomard, et al.
Published: (2023-10-01)

Induction of proteome changes involved in the cloning of mcr-1 and mcr-2 genes in Escherichia coli DH5-α strain to evaluate colistin resistance
by: Hadi Feizi, et al.
Published: (2024-03-01)

Prevalence and Some Possible Mechanisms of Colistin Resistance Among Multidrug-Resistant and Extensively Drug-Resistant Pseudomonas aeruginosa
by: Abd El-Baky RM, et al.
Published: (2020-02-01)

The Prevalence of Colistin Resistant Strains and Antibiotic Resistance Gene Profiles in Funan River, China
by: Hongmei Tuo, et al.
Published: (2018-12-01)

Detection of mcr-1-mediated resistance to polymyxins in Enterobacterales using colistin disk chelator application
by: Azyzov I.S., et al.
Published: (2022-09-01)

Co-Occurrence of the mcr-1.1 and mcr-3.7 Genes in a Multidrug-Resistant Escherichia coli Isolate from China
by: Du C, et al.
Published: (2020-10-01)

Genomic characterisation of an mcr-1 and mcr-3-producing Escherichia coli strain isolated from pigs in France
by: Afaf Hamame, et al.
Published: (2022-03-01)

Synergistic antibacterial activity of baicalin and EDTA in combination with colistin against colistin-resistant Salmonella
by: Xiao-Die Cui, et al.
Published: (2023-02-01)

Coproduction Of MCR-9 And NDM-1 By Colistin-Resistant Enterobacter hormaechei Isolated From Bloodstream Infection
by: Yuan Y, et al.
Published: (2019-09-01)

Emergence of plasmid-mediated colistin resistance mcr-3.5 gene in Citrobacter amalonaticus and Citrobacter sedlakii isolated from healthy individual in Thailand
by: Thanawat Phuadraksa, et al.
Published: (2023-01-01)

Colistin-resistance mcr genes in Klebsiella pneumoniae from companion animals
by: Guizhen Wang, et al.
Published: (2021-06-01)

Occurrence and Characteristics of Mcrs among Gram-Negative Bacteria Causing Bloodstream Infections of Infant Inpatients between 2006 and 2019 in China
by: Lijuan Wu, et al.
Published: (2022-02-01)

Plasmid-mediated colistin resistance from fresh meat and slaughtered animals in the Czech Republic: nation-wide surveillance 2020–2021
by: Petra Sismova, et al.
Published: (2023-10-01)

The first report of the mobile colistin resistance gene, mcr-10.1, in Kenya and a novel mutation in the phoQ gene (S244T) in a colistin-resistant Enterobacter cloacae clinical isolate
by: Collins Kigen, et al.
Published: (2024-02-01)

Is Africa ready for mobile colistin resistance threat?
by: Madubuike Umunna Anyanwu, et al.
Published: (2021-01-01)

Structural‐Activity Relationship‐Inspired the Discovery of Saturated Fatty Acids as Novel Colistin Enhancers
by: Jinju Cai, et al.
Published: (2023-10-01)

SuperPolymyxin™ Medium for the Screening of Colistin-Resistant Gram-Negative Bacteria in Stool Samples
by: Sara M. Przybysz, et al.
Published: (2018-11-01)

Comparative evaluation of the UMIC Colistine kit to assess MIC of colistin of gram-negative rods
by: Lucie Bardet, et al.
Published: (2019-03-01)

Putative mobilized colistin resistance genes in the human gut microbiome
by: Bruno G. N. Andrade, et al.
Published: (2021-07-01)

In vitro reduction of colistin susceptibility and comparative genomics reveals multiple differences between MCR-positive and MCR-negative colistin-resistant Escherichia coli
by: Luo Q, et al.
Published: (2019-06-01)

Plasmid-mediated colistin resistance among human clinical Enterobacterales isolates: national surveillance in the Czech Republic
by: Marketa Zelendova, et al.
Published: (2023-04-01)

Mobile colistin resistance: Prevalence, mechanisms, and current detection methods
by: Nguyen Quang Truong, et al.
Published: (2022-11-01)

Screening of colistin-resistant bacteria in livestock animals from France
by: Afaf Hamame, et al.
Published: (2022-11-01)

Chromosomally and Plasmid-Located mcr in Salmonella from Animals and Food Products in China
by: Cai-Yue Mei, et al.
Published: (2022-12-01)