High quality RNA extraction from Pseudomonas aeruginosa and other bacteria with a novel rapid and cost-effective method

High quality RNA extraction from Pseudomonas aeruginosa and other bacteria with a novel rapid and cost-effective method

AbstractHigh-quality mRNA extraction is essential for gene expression assays. In this study, we developed a rapid method (20 min) named FACTS for the extraction of intact RNA from Pseudomonas aeruginosa and compared its performance to established rapid techniques like RNAsnap and CiAR. The RNA integ...

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Bibliographic Details
Main Authors: Ivan Stoikov, Ivan Nikolaev Ivanov, Deyan Donchev, Elina Dobreva, Rumyana Hristova, Stefana Sabtcheva
Format: Article
Language:English
Published: Taylor & Francis Group 2023-12-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Pseudomonas
RNA extraction
RIN
gene expression
Gram-positive
RT-PCR
Online Access:https://www.tandfonline.com/doi/10.1080/13102818.2023.2234502

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