Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations
The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2008-12-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/8/12/7894/ |
| _version_ | 1811307034522419200 |
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| author | Dorothee Grieshaber Thomas Hirt Janos Vörös Victoria de Lange Zhihua Lu |
| author_facet | Dorothee Grieshaber Thomas Hirt Janos Vörös Victoria de Lange Zhihua Lu |
| author_sort | Dorothee Grieshaber |
| collection | DOAJ |
| description | The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesicles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes. |
| first_indexed | 2024-04-13T08:56:16Z |
| format | Article |
| id | doaj.art-31a0b19b6e6148e4813ca7d32ea784c2 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-13T08:56:16Z |
| publishDate | 2008-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-31a0b19b6e6148e4813ca7d32ea784c22022-12-22T02:53:16ZengMDPI AGSensors1424-82202008-12-018127894790310.3390/s8127894Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen ConcentrationsDorothee GrieshaberThomas HirtJanos VörösVictoria de LangeZhihua LuThe sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesicles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes.http://www.mdpi.com/1424-8220/8/12/7894/High sensitivityantigen detectionbiosensorlipid vesiclesquartz crystal microbalance |
| spellingShingle | Dorothee Grieshaber Thomas Hirt Janos Vörös Victoria de Lange Zhihua Lu Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations Sensors High sensitivity antigen detection biosensor lipid vesicles quartz crystal microbalance |
| title | Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations |
| title_full | Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations |
| title_fullStr | Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations |
| title_full_unstemmed | Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations |
| title_short | Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations |
| title_sort | vesicles for signal amplification in a biosensor for the detection of low antigen concentrations |
| topic | High sensitivity antigen detection biosensor lipid vesicles quartz crystal microbalance |
| url | http://www.mdpi.com/1424-8220/8/12/7894/ |
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