Randomly amplified polymorphic DNA analysis of Staphylococcus chromogenes isolated from bovine and bubaline mastitis in Karnataka
Background and Aim: In recent times, non-aureus staphylococci (NAS) have emerged as the major organisms isolated from mastitis cases in dairy animals, with a predominance of Staphylococcus epidermidis and Staphylococcus chromogenes. As compared to Staphylococcus aureus, much less is known about the...
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Veterinary World
2021-01-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.14/January-2021/37.pdf |
| _version_ | 1818739999103778816 |
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| author | P. Sheela Malathi Shekar Shrikrishna Isloor D. Rathnamma B. M. Veeregowda M. L. Satyanarayana S. Sundareshan B. E. Shambulingappa Nagendra R. Hegde |
| author_facet | P. Sheela Malathi Shekar Shrikrishna Isloor D. Rathnamma B. M. Veeregowda M. L. Satyanarayana S. Sundareshan B. E. Shambulingappa Nagendra R. Hegde |
| author_sort | P. Sheela |
| collection | DOAJ |
| description | Background and Aim: In recent times, non-aureus staphylococci (NAS) have emerged as the major organisms isolated from mastitis cases in dairy animals, with a predominance of Staphylococcus epidermidis and Staphylococcus chromogenes. As compared to Staphylococcus aureus, much less is known about the molecular types or the spatiotemporal epidemiology of these NAS species. In the present study, randomly amplified polymorphic DNA (RAPD) was employed to detect genetic polymorphisms, intraspecies diversity, and epidemiology of S. chromogenes strains (n=37) isolated from bovine and bubaline mastitis cases in the state of Karnataka.
Materials and Methods: Thirty-seven S. chromogenes isolates (14 from bovines and 23 from bubaline) isolated from subclinical mastitis cases, from organized and unorganized sectors, were subjected to RAPD typing. Further, methicillin resistance was determined by cefoxitin disk diffusion method.
Results: The amplified DNA fragments ranged from 150 to 3000 base pairs and yielded several RAPD profiles. Further analysis using Digital Image Correlation Engine correlation coefficient and UPGMA method showed that the 37 isolates could be classified into 12 distinct RAPD types (A to L) at 62% similarity (D=0.889). Four of the most predominant RAPD types, B, A, C, and E, in that order, and together, represented 65% of the isolates. High diversity was observed among the isolates both within farms and between geographic locations. Most of the isolates exhibited methicillin resistance. This is the first such report from India.
Conclusion: In the absence of defined multilocus sequence type protocols or sufficient sequences available in the public domain, RAPD can be employed to determine genetic diversity of S. chromogenes isolates. |
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| issn | 0972-8988 2231-0916 |
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| spelling | doaj.art-31a1d1e551434a0a9a4b3658111ebb2e2022-12-21T21:25:32ZengVeterinary WorldVeterinary World0972-89882231-09162021-01-0114128529110.14202/vetworld.2021.285-291Randomly amplified polymorphic DNA analysis of Staphylococcus chromogenes isolated from bovine and bubaline mastitis in KarnatakaP. Sheela0https://orcid.org/0000-0002-3518-9443Malathi Shekar1https://orcid.org/0000-0001-9193-9416Shrikrishna Isloor2https://orcid.org/0000-0002-3264-3874D. Rathnamma3https://orcid.org/0000-0002-2266-6853B. M. Veeregowda4https://orcid.org/0000-0002-0532-662XM. L. Satyanarayana5S. Sundareshan6https://orcid.org/0000-0003-4573-9223B. E. Shambulingappa7https://orcid.org/0000-0003-3226-6084Nagendra R. Hegde8https://orcid.org/0000-0001-5260-2159Department of Veterinary Microbiology, Veterinary College, Shivamogga, Karnataka Veterinary Animal and Fisheries Sciences University, Shivamogga, Karnataka, India.Bioinformatics Centre, Department of Aquatic Animal Health Management, College of Fisheries, Mangaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Mangaluru, Karnataka, India.Department of Microbiology, Veterinary College, Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, India.Department of Microbiology, Veterinary College, Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, India.Department of Microbiology, Veterinary College, Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, India.Department of Pathology, Veterinary College, Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, India.Department of Veterinary Microbiology, Veterinary College, Shivamogga, Karnataka Veterinary Animal and Fisheries Sciences University, Shivamogga, Karnataka, India.Department of Veterinary Microbiology, Veterinary College, Shivamogga, Karnataka Veterinary Animal and Fisheries Sciences University, Shivamogga, Karnataka, India.National Institute of Animal Biotechnology, Gachibowli, Hyderabad, Telangana, India.Background and Aim: In recent times, non-aureus staphylococci (NAS) have emerged as the major organisms isolated from mastitis cases in dairy animals, with a predominance of Staphylococcus epidermidis and Staphylococcus chromogenes. As compared to Staphylococcus aureus, much less is known about the molecular types or the spatiotemporal epidemiology of these NAS species. In the present study, randomly amplified polymorphic DNA (RAPD) was employed to detect genetic polymorphisms, intraspecies diversity, and epidemiology of S. chromogenes strains (n=37) isolated from bovine and bubaline mastitis cases in the state of Karnataka. Materials and Methods: Thirty-seven S. chromogenes isolates (14 from bovines and 23 from bubaline) isolated from subclinical mastitis cases, from organized and unorganized sectors, were subjected to RAPD typing. Further, methicillin resistance was determined by cefoxitin disk diffusion method. Results: The amplified DNA fragments ranged from 150 to 3000 base pairs and yielded several RAPD profiles. Further analysis using Digital Image Correlation Engine correlation coefficient and UPGMA method showed that the 37 isolates could be classified into 12 distinct RAPD types (A to L) at 62% similarity (D=0.889). Four of the most predominant RAPD types, B, A, C, and E, in that order, and together, represented 65% of the isolates. High diversity was observed among the isolates both within farms and between geographic locations. Most of the isolates exhibited methicillin resistance. This is the first such report from India. Conclusion: In the absence of defined multilocus sequence type protocols or sufficient sequences available in the public domain, RAPD can be employed to determine genetic diversity of S. chromogenes isolates.http://www.veterinaryworld.org/Vol.14/January-2021/37.pdfmastitismethicillin resistancenon-aureus staphylococcirandomly amplified polymorphic dnastaphylococcus chromogenes |
| spellingShingle | P. Sheela Malathi Shekar Shrikrishna Isloor D. Rathnamma B. M. Veeregowda M. L. Satyanarayana S. Sundareshan B. E. Shambulingappa Nagendra R. Hegde Randomly amplified polymorphic DNA analysis of Staphylococcus chromogenes isolated from bovine and bubaline mastitis in Karnataka Veterinary World mastitis methicillin resistance non-aureus staphylococci randomly amplified polymorphic dna staphylococcus chromogenes |
| title | Randomly amplified polymorphic DNA analysis of Staphylococcus chromogenes isolated from bovine and bubaline mastitis in Karnataka |
| title_full | Randomly amplified polymorphic DNA analysis of Staphylococcus chromogenes isolated from bovine and bubaline mastitis in Karnataka |
| title_fullStr | Randomly amplified polymorphic DNA analysis of Staphylococcus chromogenes isolated from bovine and bubaline mastitis in Karnataka |
| title_full_unstemmed | Randomly amplified polymorphic DNA analysis of Staphylococcus chromogenes isolated from bovine and bubaline mastitis in Karnataka |
| title_short | Randomly amplified polymorphic DNA analysis of Staphylococcus chromogenes isolated from bovine and bubaline mastitis in Karnataka |
| title_sort | randomly amplified polymorphic dna analysis of staphylococcus chromogenes isolated from bovine and bubaline mastitis in karnataka |
| topic | mastitis methicillin resistance non-aureus staphylococci randomly amplified polymorphic dna staphylococcus chromogenes |
| url | http://www.veterinaryworld.org/Vol.14/January-2021/37.pdf |
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