A novel indocyanine green-based fluorescent marker for guiding surgical tumor resection
Surgical tumor resection is a common approach to cancer treatment. India Ink tattoos are widely used to aid tumor resection by localizing and mapping the tumor edge at the surface. However, India Ink tattoos are easily obscured during electrosurgical resection, and fade in intensity over time. In th...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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World Scientific Publishing
2021-05-01
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| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | http://www.worldscientific.com/doi/epdf/10.1142/S1793545821500139 |
| _version_ | 1818735173411274752 |
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| author | Jiawei Ge Justin D. Opfermann Hamed Saeidi Katherine A. Huenerberg Christopher D. Badger Jaepyeong Cha Martin J. Schnermann Arjun S. Joshi Axel Krieger |
| author_facet | Jiawei Ge Justin D. Opfermann Hamed Saeidi Katherine A. Huenerberg Christopher D. Badger Jaepyeong Cha Martin J. Schnermann Arjun S. Joshi Axel Krieger |
| author_sort | Jiawei Ge |
| collection | DOAJ |
| description | Surgical tumor resection is a common approach to cancer treatment. India Ink tattoos are widely used to aid tumor resection by localizing and mapping the tumor edge at the surface. However, India Ink tattoos are easily obscured during electrosurgical resection, and fade in intensity over time. In this work, a novel near-infrared (NIR) fluorescent marker is introduced as an alternative. The NIR marker was made by mixing indocyanine green (ICG), biocompatible cyanoacrylate, and acetone. The marking strategy was evaluated in a chronic ex vivo feasibility study using porcine tissues, followed by a chronic in vivo mouse study while compared with India Ink. In both studies, signal-to-noise (SNR) ratios and dimensions of the NIR markers and/or India Ink over the study period were calculated and reported. Electrocautery was performed on the last day of the mouse study after mice were euthanized, and SNR ratios and dimensions were quantified and compared. Biopsy was performed at all injection sites and slides were examined by a pathologist. The proposed NIR marker achieved (i) consistent visibility in the 26-day feasibility study and (ii) improved durability, visibility, and biocompatibility when compared to traditional India Ink over the six-week period in an in vivo mouse model. These effects persist after electrocautery whereas the India Ink markers were obscured. The use of a NIR fluorescent presurgical marking strategy has the potential for intraoperative tracking during long-term treatment protocols. |
| first_indexed | 2024-12-18T00:17:03Z |
| format | Article |
| id | doaj.art-31a44133efc24966a405347e88f4200b |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-12-18T00:17:03Z |
| publishDate | 2021-05-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-31a44133efc24966a405347e88f4200b2022-12-21T21:27:29ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052021-05-011432150013-12150013-1110.1142/S179354582150013910.1142/S1793545821500139A novel indocyanine green-based fluorescent marker for guiding surgical tumor resectionJiawei Ge0Justin D. Opfermann1Hamed Saeidi2Katherine A. Huenerberg3Christopher D. Badger4Jaepyeong Cha5Martin J. Schnermann6Arjun S. Joshi7Axel Krieger8Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USADepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USADepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USADepartment of Pathology, George Washington University, Washington, DC 20052, USADivision of Otolaryngology - Head and Neck Surgery, George Washington University, Washington, DC 20052, USASheikh Zayed Institute for Pediatric Surgical Innovation, Children’s National Hospital, Washington, DC 20010, USAChemical Biology Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USADivision of Otolaryngology - Head and Neck Surgery, George Washington University, Washington, DC 20052, USADepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USASurgical tumor resection is a common approach to cancer treatment. India Ink tattoos are widely used to aid tumor resection by localizing and mapping the tumor edge at the surface. However, India Ink tattoos are easily obscured during electrosurgical resection, and fade in intensity over time. In this work, a novel near-infrared (NIR) fluorescent marker is introduced as an alternative. The NIR marker was made by mixing indocyanine green (ICG), biocompatible cyanoacrylate, and acetone. The marking strategy was evaluated in a chronic ex vivo feasibility study using porcine tissues, followed by a chronic in vivo mouse study while compared with India Ink. In both studies, signal-to-noise (SNR) ratios and dimensions of the NIR markers and/or India Ink over the study period were calculated and reported. Electrocautery was performed on the last day of the mouse study after mice were euthanized, and SNR ratios and dimensions were quantified and compared. Biopsy was performed at all injection sites and slides were examined by a pathologist. The proposed NIR marker achieved (i) consistent visibility in the 26-day feasibility study and (ii) improved durability, visibility, and biocompatibility when compared to traditional India Ink over the six-week period in an in vivo mouse model. These effects persist after electrocautery whereas the India Ink markers were obscured. The use of a NIR fluorescent presurgical marking strategy has the potential for intraoperative tracking during long-term treatment protocols.http://www.worldscientific.com/doi/epdf/10.1142/S1793545821500139near-infrared fluorescent markersurgical tumor resectionfluorescence-guided surgeryelectrosurgeryindia ink |
| spellingShingle | Jiawei Ge Justin D. Opfermann Hamed Saeidi Katherine A. Huenerberg Christopher D. Badger Jaepyeong Cha Martin J. Schnermann Arjun S. Joshi Axel Krieger A novel indocyanine green-based fluorescent marker for guiding surgical tumor resection Journal of Innovative Optical Health Sciences near-infrared fluorescent marker surgical tumor resection fluorescence-guided surgery electrosurgery india ink |
| title | A novel indocyanine green-based fluorescent marker for guiding surgical tumor resection |
| title_full | A novel indocyanine green-based fluorescent marker for guiding surgical tumor resection |
| title_fullStr | A novel indocyanine green-based fluorescent marker for guiding surgical tumor resection |
| title_full_unstemmed | A novel indocyanine green-based fluorescent marker for guiding surgical tumor resection |
| title_short | A novel indocyanine green-based fluorescent marker for guiding surgical tumor resection |
| title_sort | novel indocyanine green based fluorescent marker for guiding surgical tumor resection |
| topic | near-infrared fluorescent marker surgical tumor resection fluorescence-guided surgery electrosurgery india ink |
| url | http://www.worldscientific.com/doi/epdf/10.1142/S1793545821500139 |
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