Functional interplay between antagonistic bacteria and Rhizoctonia solani in the tomato plant rhizosphere
Microbial interactions with plant roots play an imperial role in tomato plant growth and defense against the Rhizoctonia solani. This study performed a field experiment with two antagonistic bacteria (Pseudomonas and Bacillus) inoculated in healthy and Rhizoctonia solani treated soil in tomato rhizo...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.990850/full |
| _version_ | 1811258707123634176 |
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| author | Manoj Kumar Solanki Anjali Chandrol Solanki Shalini Rai Supriya Srivastava Brijendra Kumar Kashyap Praveen Kumar Divvela Sudheer Kumar Mahesh S. Yandigeri Prem Lal Kashyap Alok Kumar Shrivastava Baber Ali Shahid Khan Shahid Khan Mariusz Jaremko Kamal Ahmad Qureshi |
| author_facet | Manoj Kumar Solanki Anjali Chandrol Solanki Shalini Rai Supriya Srivastava Brijendra Kumar Kashyap Praveen Kumar Divvela Sudheer Kumar Mahesh S. Yandigeri Prem Lal Kashyap Alok Kumar Shrivastava Baber Ali Shahid Khan Shahid Khan Mariusz Jaremko Kamal Ahmad Qureshi |
| author_sort | Manoj Kumar Solanki |
| collection | DOAJ |
| description | Microbial interactions with plant roots play an imperial role in tomato plant growth and defense against the Rhizoctonia solani. This study performed a field experiment with two antagonistic bacteria (Pseudomonas and Bacillus) inoculated in healthy and Rhizoctonia solani treated soil in tomato rhizosphere to understand the metabolic pattern and microbial function during plant disease suppression. In the present study, we assessed soil and microbial enzymes, bacterial and fungal cell forming unit (CFU), and carbon utilization profiling through Bio-Eco plates of rhizoplane samples. Antagonist bacteria and pathogen interaction significantly (p < 0.05) influenced the bacterial count, soil enzymes (chitinase and glucanase), and bacterial function (siderophore and chitinase production). These results indicated that these variables had an imperial role in disease suppression during plant development. Furthermore, the metabolic profiling showed that carbon source utilization enhanced under fruit development and ripening stages. These results suggested that carbon sources were essential in plant/pathogen/antagonist interaction. Substrates like β-methyl-D-glucoside, D-mannitol, D-galacturonic acid, N-acetyl-D-glucosamine, and phenylethylamine strongly connect with the suppuration of root rot disease. These carbon sources may help to propagate a healthy microbial community to reduce the pathogen invasion in the plant root system, and these carbon sources can be stimulators of antagonists against pathogens in the future. |
| first_indexed | 2024-04-12T18:18:37Z |
| format | Article |
| id | doaj.art-31a59b2a74e0408c96b991978c5338b7 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T18:18:37Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-31a59b2a74e0408c96b991978c5338b72022-12-22T03:21:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.990850990850Functional interplay between antagonistic bacteria and Rhizoctonia solani in the tomato plant rhizosphereManoj Kumar Solanki0Anjali Chandrol Solanki1Shalini Rai2Supriya Srivastava3Brijendra Kumar Kashyap4Praveen Kumar Divvela5Sudheer Kumar6Mahesh S. Yandigeri7Prem Lal Kashyap8Alok Kumar Shrivastava9Baber Ali10Shahid Khan11Shahid Khan12Mariusz Jaremko13Kamal Ahmad Qureshi14Faculty of Natural Sciences, Plant Cytogenetics and Molecular Biology Group, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, Katowice, PolandDepartment of Agriculture Science, Mansarovar Global University, Bhopal, MP, IndiaDepartment of Biotechnology, Society of Higher Education and Practical Application (SHEPA), Varanasi, UP, IndiaDepartment of Health Informatics, College of Public Health and Health Informatics, Qassim University, Al Bukayriyah, Saudi ArabiaDepartment of Biotechnology Engineering, Institute of Engineering and Technology, Bundelkhand University, Jhansi, UP, IndiaContec Global Agro Limited, Abuja, NigeriaIndian Institute of Wheat and Barley Research (ICAR), Karnal, HR, IndiaNational Bureau of Agricultural Insect Resources (ICAR), Bengaluru, KA, IndiaIndian Institute of Wheat and Barley Research (ICAR), Karnal, HR, IndiaNational Bureau of Agriculturally Important Microorganisms (ICAR), Kusmaur, UP, India0Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan1Department of Agriculture, University of Swabi, Swabi, Pakistan2Department of Plant Breeding and Genetics, University of Agriculture Swat, Peshawar, Pakistan3Smart-Health Initiative (SHI) and Red Sea Research Center (RSRC), Division of Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia4Department of Pharmaceutics, Unaizah College of Pharmacy, Qassim University, Unaizah, Saudi ArabiaMicrobial interactions with plant roots play an imperial role in tomato plant growth and defense against the Rhizoctonia solani. This study performed a field experiment with two antagonistic bacteria (Pseudomonas and Bacillus) inoculated in healthy and Rhizoctonia solani treated soil in tomato rhizosphere to understand the metabolic pattern and microbial function during plant disease suppression. In the present study, we assessed soil and microbial enzymes, bacterial and fungal cell forming unit (CFU), and carbon utilization profiling through Bio-Eco plates of rhizoplane samples. Antagonist bacteria and pathogen interaction significantly (p < 0.05) influenced the bacterial count, soil enzymes (chitinase and glucanase), and bacterial function (siderophore and chitinase production). These results indicated that these variables had an imperial role in disease suppression during plant development. Furthermore, the metabolic profiling showed that carbon source utilization enhanced under fruit development and ripening stages. These results suggested that carbon sources were essential in plant/pathogen/antagonist interaction. Substrates like β-methyl-D-glucoside, D-mannitol, D-galacturonic acid, N-acetyl-D-glucosamine, and phenylethylamine strongly connect with the suppuration of root rot disease. These carbon sources may help to propagate a healthy microbial community to reduce the pathogen invasion in the plant root system, and these carbon sources can be stimulators of antagonists against pathogens in the future.https://www.frontiersin.org/articles/10.3389/fmicb.2022.990850/fullpseudomonasbacillusBIOLOGcommunity-level physiological profiledisease incidence |
| spellingShingle | Manoj Kumar Solanki Anjali Chandrol Solanki Shalini Rai Supriya Srivastava Brijendra Kumar Kashyap Praveen Kumar Divvela Sudheer Kumar Mahesh S. Yandigeri Prem Lal Kashyap Alok Kumar Shrivastava Baber Ali Shahid Khan Shahid Khan Mariusz Jaremko Kamal Ahmad Qureshi Functional interplay between antagonistic bacteria and Rhizoctonia solani in the tomato plant rhizosphere Frontiers in Microbiology pseudomonas bacillus BIOLOG community-level physiological profile disease incidence |
| title | Functional interplay between antagonistic bacteria and Rhizoctonia solani in the tomato plant rhizosphere |
| title_full | Functional interplay between antagonistic bacteria and Rhizoctonia solani in the tomato plant rhizosphere |
| title_fullStr | Functional interplay between antagonistic bacteria and Rhizoctonia solani in the tomato plant rhizosphere |
| title_full_unstemmed | Functional interplay between antagonistic bacteria and Rhizoctonia solani in the tomato plant rhizosphere |
| title_short | Functional interplay between antagonistic bacteria and Rhizoctonia solani in the tomato plant rhizosphere |
| title_sort | functional interplay between antagonistic bacteria and rhizoctonia solani in the tomato plant rhizosphere |
| topic | pseudomonas bacillus BIOLOG community-level physiological profile disease incidence |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.990850/full |
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