Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Suppresses Melanogenesis Induced by α-MSH: In Vitro and In Vivo
Diphlorethohydroxycarmalol (DPHC) isolated from <i>Ishige okamurae</i> (IO) showed potential whitening effects against UV-B radiation. However, the components of IO as well as their molecular mechanism against α-melanocyte-stimulating hormone (α-MSH) have not yet been investigated. Thus,...
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MDPI AG
2020-09-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/9/470 |
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| author | Xining Li Hye-Won Yang Yunfei Jiang Jae-Young Oh You-Jin Jeon Bomi Ryu |
| author_facet | Xining Li Hye-Won Yang Yunfei Jiang Jae-Young Oh You-Jin Jeon Bomi Ryu |
| author_sort | Xining Li |
| collection | DOAJ |
| description | Diphlorethohydroxycarmalol (DPHC) isolated from <i>Ishige okamurae</i> (IO) showed potential whitening effects against UV-B radiation. However, the components of IO as well as their molecular mechanism against α-melanocyte-stimulating hormone (α-MSH) have not yet been investigated. Thus, this study aimed to investigate the inhibitory effects of Ishophloroglucin A (IPA), a phlorotannin isolated from brown algae IO, and its crude extract (IOE), in melanogenesis in vivo in an α-MSH-induced zebrafish model and in B16F10 melanoma cells in vitro. Molecular docking studies of the phlorotannins were carried out to determine their inhibitory effects and to elucidate their mode of interaction with tyrosinase, a glycoprotein related to melanogenesis. In addition, morphological changes and melanin content decreased in the α-MSH-induced zebrafish model after IPA and IOE treatment. Furthermore, Western blotting results revealed that IPA upregulated the extracellular related protein expression in α-MSH-stimulated B16F10 cells. Hence, these results suggest that IPA isolated from IOE has a potential for use in the pharmaceutical and cosmetic industries. |
| first_indexed | 2024-03-10T16:14:18Z |
| format | Article |
| id | doaj.art-31a74ed8d75c4eb79628c5978d9f8714 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T16:14:18Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-31a74ed8d75c4eb79628c5978d9f87142023-11-20T14:08:25ZengMDPI AGMarine Drugs1660-33972020-09-0118947010.3390/md18090470Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Suppresses Melanogenesis Induced by α-MSH: In Vitro and In VivoXining Li0Hye-Won Yang1Yunfei Jiang2Jae-Young Oh3You-Jin Jeon4Bomi Ryu5Department of Marine Life Sciences, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 63243, KoreaDiphlorethohydroxycarmalol (DPHC) isolated from <i>Ishige okamurae</i> (IO) showed potential whitening effects against UV-B radiation. However, the components of IO as well as their molecular mechanism against α-melanocyte-stimulating hormone (α-MSH) have not yet been investigated. Thus, this study aimed to investigate the inhibitory effects of Ishophloroglucin A (IPA), a phlorotannin isolated from brown algae IO, and its crude extract (IOE), in melanogenesis in vivo in an α-MSH-induced zebrafish model and in B16F10 melanoma cells in vitro. Molecular docking studies of the phlorotannins were carried out to determine their inhibitory effects and to elucidate their mode of interaction with tyrosinase, a glycoprotein related to melanogenesis. In addition, morphological changes and melanin content decreased in the α-MSH-induced zebrafish model after IPA and IOE treatment. Furthermore, Western blotting results revealed that IPA upregulated the extracellular related protein expression in α-MSH-stimulated B16F10 cells. Hence, these results suggest that IPA isolated from IOE has a potential for use in the pharmaceutical and cosmetic industries.https://www.mdpi.com/1660-3397/18/9/470Ishige okamuraemelanogenesisα-MSHzebrafish |
| spellingShingle | Xining Li Hye-Won Yang Yunfei Jiang Jae-Young Oh You-Jin Jeon Bomi Ryu Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Suppresses Melanogenesis Induced by α-MSH: In Vitro and In Vivo Marine Drugs Ishige okamurae melanogenesis α-MSH zebrafish |
| title | Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Suppresses Melanogenesis Induced by α-MSH: In Vitro and In Vivo |
| title_full | Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Suppresses Melanogenesis Induced by α-MSH: In Vitro and In Vivo |
| title_fullStr | Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Suppresses Melanogenesis Induced by α-MSH: In Vitro and In Vivo |
| title_full_unstemmed | Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Suppresses Melanogenesis Induced by α-MSH: In Vitro and In Vivo |
| title_short | Ishophloroglucin A Isolated from <i>Ishige okamurae</i> Suppresses Melanogenesis Induced by α-MSH: In Vitro and In Vivo |
| title_sort | ishophloroglucin a isolated from i ishige okamurae i suppresses melanogenesis induced by α msh in vitro and in vivo |
| topic | Ishige okamurae melanogenesis α-MSH zebrafish |
| url | https://www.mdpi.com/1660-3397/18/9/470 |
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