Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics
Abstract Even though Helicobacter pylori (H. pylori) is a serious pathogen, its origin is unknown. Poultry (Chicken, Turkey, Quebec, Goose, and Ostrich) are consumed as a regular protein source by a large number of people across the world; therefore, sanitary ways of delivering poultry for food are...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-38374-5 |
| _version_ | 1797778890677026816 |
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| author | Tohid Piri-Gharaghie Ghazal Ghajari Shakiba Tolou-Shikhzadeh-Yazdi Mona Aghassizadeh-Sherbaf Sahar Khorsand-Dehkordi |
| author_facet | Tohid Piri-Gharaghie Ghazal Ghajari Shakiba Tolou-Shikhzadeh-Yazdi Mona Aghassizadeh-Sherbaf Sahar Khorsand-Dehkordi |
| author_sort | Tohid Piri-Gharaghie |
| collection | DOAJ |
| description | Abstract Even though Helicobacter pylori (H. pylori) is a serious pathogen, its origin is unknown. Poultry (Chicken, Turkey, Quebec, Goose, and Ostrich) are consumed as a regular protein source by a large number of people across the world; therefore, sanitary ways of delivering poultry for food are important for global health. As a result, we looked at the distribution of the pathogenicity cagA, vacA, babA2, oipA, and iceA in H. pylori isolates in poultry meat, as well as their antimicrobial resistance. Wilkins Chalgren anaerobic bacterial medium was used to cultivate 320 raw poultry specimens. Disk diffusion and Multiplex-PCR were used to investigate antimicrobial resistance and genotyping patterns, separately. H. pylori was found in 20 of 320 (6.25%) raw poultry samples. The highest incidence of H. pylori was found in chicken raw meat (15%), whereas the fewest was found in Goose and Quebec (0.00%). Resistance to ampicillin (85%), tetracycline (85%), and amoxicillin (75%) were greatest in H. pylori isolates. The percentage of H. pylori isolates with a MAR value of more than 0.2 was 17/20 (85%). The most prevalent genotypes discovered were VacA s1a (75%), m1a (75%), s2 (70%) and m2 (65%), and cagA (60%). The most typically discovered genotype patterns were s1am1a (45%), s2m1a (45%), and s2m2 (30%). BabA2, OipA + , and OipA− genotypes were found in 40%, 30%, and 30% of the population. In summary, the poultry flesh was polluted by H. pylori, with the babA2, vacA, and cagA genotypes being more prevalent. The simultaneous occurrence of vacA, cagA, iceA, oipA, and babA2 genotypes in antibiotic-resistant H. pylori bacteria implies a serious public health concern about raw poultry eating. In the future, researchers should look into H. pylori's resistance to multiple antibacterial drugs in Iran. |
| first_indexed | 2024-03-12T23:23:03Z |
| format | Article |
| id | doaj.art-31a80fd312d241b596083ef9ec96bcc1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T23:23:03Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-31a80fd312d241b596083ef9ec96bcc12023-07-16T11:17:34ZengNature PortfolioScientific Reports2045-23222023-07-0113111110.1038/s41598-023-38374-5Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristicsTohid Piri-Gharaghie0Ghazal Ghajari1Shakiba Tolou-Shikhzadeh-Yazdi2Mona Aghassizadeh-Sherbaf3Sahar Khorsand-Dehkordi4Biotechnology Research Center, Faculty of Basic Sciences, Islamic Azad University, East-Tehran BranchDepartment of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi UniversityDepartment of Biology, Faculty of Sciences, Islamic Azad University, Mashhad BranchDepartment of Biology, Faculty of Basic Sciences, Islamic Azad University, East-Tehran BranchDepartment of Biology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord BranchAbstract Even though Helicobacter pylori (H. pylori) is a serious pathogen, its origin is unknown. Poultry (Chicken, Turkey, Quebec, Goose, and Ostrich) are consumed as a regular protein source by a large number of people across the world; therefore, sanitary ways of delivering poultry for food are important for global health. As a result, we looked at the distribution of the pathogenicity cagA, vacA, babA2, oipA, and iceA in H. pylori isolates in poultry meat, as well as their antimicrobial resistance. Wilkins Chalgren anaerobic bacterial medium was used to cultivate 320 raw poultry specimens. Disk diffusion and Multiplex-PCR were used to investigate antimicrobial resistance and genotyping patterns, separately. H. pylori was found in 20 of 320 (6.25%) raw poultry samples. The highest incidence of H. pylori was found in chicken raw meat (15%), whereas the fewest was found in Goose and Quebec (0.00%). Resistance to ampicillin (85%), tetracycline (85%), and amoxicillin (75%) were greatest in H. pylori isolates. The percentage of H. pylori isolates with a MAR value of more than 0.2 was 17/20 (85%). The most prevalent genotypes discovered were VacA s1a (75%), m1a (75%), s2 (70%) and m2 (65%), and cagA (60%). The most typically discovered genotype patterns were s1am1a (45%), s2m1a (45%), and s2m2 (30%). BabA2, OipA + , and OipA− genotypes were found in 40%, 30%, and 30% of the population. In summary, the poultry flesh was polluted by H. pylori, with the babA2, vacA, and cagA genotypes being more prevalent. The simultaneous occurrence of vacA, cagA, iceA, oipA, and babA2 genotypes in antibiotic-resistant H. pylori bacteria implies a serious public health concern about raw poultry eating. In the future, researchers should look into H. pylori's resistance to multiple antibacterial drugs in Iran.https://doi.org/10.1038/s41598-023-38374-5 |
| spellingShingle | Tohid Piri-Gharaghie Ghazal Ghajari Shakiba Tolou-Shikhzadeh-Yazdi Mona Aghassizadeh-Sherbaf Sahar Khorsand-Dehkordi Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics Scientific Reports |
| title | Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics |
| title_full | Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics |
| title_fullStr | Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics |
| title_full_unstemmed | Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics |
| title_short | Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics |
| title_sort | helicobacter pylori strains isolated from raw poultry meat frequency and molecular characteristics |
| url | https://doi.org/10.1038/s41598-023-38374-5 |
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