Aspects of Lyophilization of Cardiac Bioimplant
The use of implants of biological origin in clinical practice has led to the search for methods of long-term storage of tissues without damaging their functional and structural characteristics. Xenografts (extracted from pericardium of pigs, horses, bulls) are drawing more and more interest. The bov...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Igor Sikorsky Kyiv Polytechnic Institute
2021-12-01
|
| Series: | Innovative Biosystems and Bioengineering |
| Subjects: | |
| Online Access: | http://ibb.kpi.ua/article/view/239725 |
| _version_ | 1818018015673843712 |
|---|---|
| author | Nataliia Shchotkina |
| author_facet | Nataliia Shchotkina |
| author_sort | Nataliia Shchotkina |
| collection | DOAJ |
| description | The use of implants of biological origin in clinical practice has led to the search for methods of long-term storage of tissues without damaging their functional and structural characteristics. Xenografts (extracted from pericardium of pigs, horses, bulls) are drawing more and more interest. The bovine pericardium is exposed to chemical and physical factors providing complete purification of tissue from cells and their components. Such scaffolds are protein (collagen) complexes that fully replicate the microstructure of the pericardial tissue. Lyophilisation ensures long-term preservation of the extracellular matrix properties. The principle of the method is in drying pre-frozen tissue, in which water is sublimated. The method is intended for storage, transportation, and the subsequent use of the bioimplant in clinical practice. However, the lyophilization process may be accompanied by various undesirable factors that can lead to denaturation of the matrix protein or loss of its functionality and structure. To preserve the natural microstructure, stabilizers or various modifications (slow/fast freezing, reducing the degree of supercooling, etc.) of the lyophilization process are applied to biological prostheses. In this review, the main processes of lyophilization of biological tissue are described, which can affect the operation of a cardiac implant. A deep understanding of the parameters of the lyophilization process is crucial for creation of stable tissue grafts and their subsequent long-term storage. |
| first_indexed | 2024-04-14T07:33:42Z |
| format | Article |
| id | doaj.art-31b290b8d0cc4a79acf1c1e739e5aff7 |
| institution | Directory Open Access Journal |
| issn | 2616-177X |
| language | English |
| last_indexed | 2024-04-14T07:33:42Z |
| publishDate | 2021-12-01 |
| publisher | Igor Sikorsky Kyiv Polytechnic Institute |
| record_format | Article |
| series | Innovative Biosystems and Bioengineering |
| spelling | doaj.art-31b290b8d0cc4a79acf1c1e739e5aff72022-12-22T02:05:44ZengIgor Sikorsky Kyiv Polytechnic InstituteInnovative Biosystems and Bioengineering2616-177X2021-12-015410.20535/ibb.2021.5.4.239725Aspects of Lyophilization of Cardiac BioimplantNataliia Shchotkina0Center for Pediatric Cardiology and Cardiac SurgeryThe use of implants of biological origin in clinical practice has led to the search for methods of long-term storage of tissues without damaging their functional and structural characteristics. Xenografts (extracted from pericardium of pigs, horses, bulls) are drawing more and more interest. The bovine pericardium is exposed to chemical and physical factors providing complete purification of tissue from cells and their components. Such scaffolds are protein (collagen) complexes that fully replicate the microstructure of the pericardial tissue. Lyophilisation ensures long-term preservation of the extracellular matrix properties. The principle of the method is in drying pre-frozen tissue, in which water is sublimated. The method is intended for storage, transportation, and the subsequent use of the bioimplant in clinical practice. However, the lyophilization process may be accompanied by various undesirable factors that can lead to denaturation of the matrix protein or loss of its functionality and structure. To preserve the natural microstructure, stabilizers or various modifications (slow/fast freezing, reducing the degree of supercooling, etc.) of the lyophilization process are applied to biological prostheses. In this review, the main processes of lyophilization of biological tissue are described, which can affect the operation of a cardiac implant. A deep understanding of the parameters of the lyophilization process is crucial for creation of stable tissue grafts and their subsequent long-term storage.http://ibb.kpi.ua/article/view/239725scaffold lyophilizationtissue engineeringbovine pericardiumextracellular matrix |
| spellingShingle | Nataliia Shchotkina Aspects of Lyophilization of Cardiac Bioimplant Innovative Biosystems and Bioengineering scaffold lyophilization tissue engineering bovine pericardium extracellular matrix |
| title | Aspects of Lyophilization of Cardiac Bioimplant |
| title_full | Aspects of Lyophilization of Cardiac Bioimplant |
| title_fullStr | Aspects of Lyophilization of Cardiac Bioimplant |
| title_full_unstemmed | Aspects of Lyophilization of Cardiac Bioimplant |
| title_short | Aspects of Lyophilization of Cardiac Bioimplant |
| title_sort | aspects of lyophilization of cardiac bioimplant |
| topic | scaffold lyophilization tissue engineering bovine pericardium extracellular matrix |
| url | http://ibb.kpi.ua/article/view/239725 |
| work_keys_str_mv | AT nataliiashchotkina aspectsoflyophilizationofcardiacbioimplant |