A New Potent Route of DNA Vaccine Inoculation: DNA-Liposome Complexes on Bare Skin Induce Antigen-Special Antibody Responses
Transcutaneous immunization is a novel strategy for genetic vaccine immunization to induce detectable antigen-special antibody in humor and mucosal. In this study, plasmid expressing hepatitis B surface antigen (pGFP-HBsAg) was encapsulated in liposome, then DNA- liposome complexes were glued on bar...
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| Format: | Article |
| Language: | English |
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MDPI AG
2003-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/8/1/120/ |
| _version_ | 1818238831380398080 |
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| author | Mingxing Duan Changxue Zheng Mingjun Deng Zheng Meng Fengfeng Zhuang Rong Liang |
| author_facet | Mingxing Duan Changxue Zheng Mingjun Deng Zheng Meng Fengfeng Zhuang Rong Liang |
| author_sort | Mingxing Duan |
| collection | DOAJ |
| description | Transcutaneous immunization is a novel strategy for genetic vaccine immunization to induce detectable antigen-special antibody in humor and mucosal. In this study, plasmid expressing hepatitis B surface antigen (pGFP-HBsAg) was encapsulated in liposome, then DNA- liposome complexes were glued on bare skin of mice ear in different dosage (50μg, 10μg and 1μg). As control, DNA- liposome complexes of pGFP-HBsAg and pGFP vector were inoculated intraperitoneally. The anti-HBsAg antibodies of serum were detected weekly by ELISA. It was found that the detectable antibodies of transcutaneous immunized mouse were elicited after four weeks, and reached a maximum at the sixth week. Even 1μg plasmid DNA in liposomes through immune skin can elicit the highest ELISA antibody titer (> 1:512) in test group, and corresponding percentage of positive response is up to 71% at sixth week, but higher amounts of plasmid DNA (50μg DNA per mice) on immune skin cannot induce higher antibody levels. The result showed that DNA- liposome complexes glued on bare skin appear to be a novel method for the administration of DNA vaccines. |
| first_indexed | 2024-12-12T12:47:54Z |
| format | Article |
| id | doaj.art-31b5f629943344f28a180021a5bc2ef5 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T12:47:54Z |
| publishDate | 2003-01-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-31b5f629943344f28a180021a5bc2ef52022-12-22T00:24:04ZengMDPI AGMolecules1420-30492003-01-018112012610.3390/80100120A New Potent Route of DNA Vaccine Inoculation: DNA-Liposome Complexes on Bare Skin Induce Antigen-Special Antibody ResponsesMingxing DuanChangxue ZhengMingjun DengZheng MengFengfeng ZhuangRong LiangTranscutaneous immunization is a novel strategy for genetic vaccine immunization to induce detectable antigen-special antibody in humor and mucosal. In this study, plasmid expressing hepatitis B surface antigen (pGFP-HBsAg) was encapsulated in liposome, then DNA- liposome complexes were glued on bare skin of mice ear in different dosage (50μg, 10μg and 1μg). As control, DNA- liposome complexes of pGFP-HBsAg and pGFP vector were inoculated intraperitoneally. The anti-HBsAg antibodies of serum were detected weekly by ELISA. It was found that the detectable antibodies of transcutaneous immunized mouse were elicited after four weeks, and reached a maximum at the sixth week. Even 1μg plasmid DNA in liposomes through immune skin can elicit the highest ELISA antibody titer (> 1:512) in test group, and corresponding percentage of positive response is up to 71% at sixth week, but higher amounts of plasmid DNA (50μg DNA per mice) on immune skin cannot induce higher antibody levels. The result showed that DNA- liposome complexes glued on bare skin appear to be a novel method for the administration of DNA vaccines.http://www.mdpi.com/1420-3049/8/1/120/genetic vaccineliposometranscutaneous immunization |
| spellingShingle | Mingxing Duan Changxue Zheng Mingjun Deng Zheng Meng Fengfeng Zhuang Rong Liang A New Potent Route of DNA Vaccine Inoculation: DNA-Liposome Complexes on Bare Skin Induce Antigen-Special Antibody Responses Molecules genetic vaccine liposome transcutaneous immunization |
| title | A New Potent Route of DNA Vaccine Inoculation: DNA-Liposome Complexes on Bare Skin Induce Antigen-Special Antibody Responses |
| title_full | A New Potent Route of DNA Vaccine Inoculation: DNA-Liposome Complexes on Bare Skin Induce Antigen-Special Antibody Responses |
| title_fullStr | A New Potent Route of DNA Vaccine Inoculation: DNA-Liposome Complexes on Bare Skin Induce Antigen-Special Antibody Responses |
| title_full_unstemmed | A New Potent Route of DNA Vaccine Inoculation: DNA-Liposome Complexes on Bare Skin Induce Antigen-Special Antibody Responses |
| title_short | A New Potent Route of DNA Vaccine Inoculation: DNA-Liposome Complexes on Bare Skin Induce Antigen-Special Antibody Responses |
| title_sort | new potent route of dna vaccine inoculation dna liposome complexes on bare skin induce antigen special antibody responses |
| topic | genetic vaccine liposome transcutaneous immunization |
| url | http://www.mdpi.com/1420-3049/8/1/120/ |
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