Molecular epidemiology analysis of symptomatic and asymptomatic norovirus infections in Chinese infants
Abstract Background Norovirus is a leading cause of acute gastroenteritis among children. Previous studies based on symptomatic infections indicated that mutations, rather than recombination drove the evolution of the norovirus ORF2. These characteristics were found in hospital-based symptomatic inf...
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | Virology Journal |
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| Online Access: | https://doi.org/10.1186/s12985-023-02024-z |
| _version_ | 1797850218943741952 |
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| author | Li-Na Chen Si-Jie Wang Song-Mei Wang Xiao-Li Fu Wen-Jing Zheng Zhi-Yong Hao Hai-Song Zhou Xin-Jiang Zhang Yu-Liang Zhao Chao Qiu Lorenz von Seidlein Tian-Yi Qiu Xuan-Yi Wang |
| author_facet | Li-Na Chen Si-Jie Wang Song-Mei Wang Xiao-Li Fu Wen-Jing Zheng Zhi-Yong Hao Hai-Song Zhou Xin-Jiang Zhang Yu-Liang Zhao Chao Qiu Lorenz von Seidlein Tian-Yi Qiu Xuan-Yi Wang |
| author_sort | Li-Na Chen |
| collection | DOAJ |
| description | Abstract Background Norovirus is a leading cause of acute gastroenteritis among children. Previous studies based on symptomatic infections indicated that mutations, rather than recombination drove the evolution of the norovirus ORF2. These characteristics were found in hospital-based symptomatic infections, whereas, asymptomatic infections are frequent and contribute significantly to transmission. Methods We conducted the first norovirus molecular epidemiology analysis covering both symptomatic and asymptomatic infections derived from a birth cohort study in the northern China. Results During the study, 14 symptomatic and 20 asymptomatic norovirus infections were detected in 32 infants. Out of the 14 strains that caused symptomatic infections, 12 strains were identified as GII.3[P12], and others were GII.4[P31]. Conversely, 17 asymptomatic infections were caused by GII.4[P31], two by GII.2[P16], and one by GII.4[P16]. Regardless of symptomatic and asymptomatic infections, the mutations were detected frequently in the ORF2 region, and almost all recombination were identified in the RdRp-ORF2 region. The majority of the mutations were located around the predefined epitope regions of P2 subdomain indicating a potential for immune evasion. Conclusion The role of symptomatic as well as asymptomatic infections in the evolution of norovirus needs to be evaluated continuously. |
| first_indexed | 2024-04-09T18:56:51Z |
| format | Article |
| id | doaj.art-31b6b84bef5c4de28ca1383fb7f548d9 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-09T18:56:51Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-31b6b84bef5c4de28ca1383fb7f548d92023-04-09T11:07:59ZengBMCVirology Journal1743-422X2023-04-0120111110.1186/s12985-023-02024-zMolecular epidemiology analysis of symptomatic and asymptomatic norovirus infections in Chinese infantsLi-Na Chen0Si-Jie Wang1Song-Mei Wang2Xiao-Li Fu3Wen-Jing Zheng4Zhi-Yong Hao5Hai-Song Zhou6Xin-Jiang Zhang7Yu-Liang Zhao8Chao Qiu9Lorenz von Seidlein10Tian-Yi Qiu11Xuan-Yi Wang12Key Laboratory of Medical Molecular Virology of MoE & MoH and Institutes of Biomedical Sciences, Fudan UniversityKey Laboratory of Medical Molecular Virology of MoE & MoH and Institutes of Biomedical Sciences, Fudan UniversityLaboratory of Molecular Biology, Training Center of Medical Experiments, School of Basic Medical Sciences, Fudan UniversityKey Laboratory of Medical Molecular Virology of MoE & MoH and Institutes of Biomedical Sciences, Fudan UniversityKey Laboratory of Medical Molecular Virology of MoE & MoH and Institutes of Biomedical Sciences, Fudan UniversityZhengding County Center for Disease Control and PreventionZhengding County Center for Disease Control and PreventionZhengding County Center for Disease Control and PreventionHebei Province Center for Disease Control and PreventionKey Laboratory of Medical Molecular Virology of MoE & MoH and Institutes of Biomedical Sciences, Fudan UniversityMahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol UniversityInstitute of Clinical Science, ZhongShan Hospital, Fudan UniversityKey Laboratory of Medical Molecular Virology of MoE & MoH and Institutes of Biomedical Sciences, Fudan UniversityAbstract Background Norovirus is a leading cause of acute gastroenteritis among children. Previous studies based on symptomatic infections indicated that mutations, rather than recombination drove the evolution of the norovirus ORF2. These characteristics were found in hospital-based symptomatic infections, whereas, asymptomatic infections are frequent and contribute significantly to transmission. Methods We conducted the first norovirus molecular epidemiology analysis covering both symptomatic and asymptomatic infections derived from a birth cohort study in the northern China. Results During the study, 14 symptomatic and 20 asymptomatic norovirus infections were detected in 32 infants. Out of the 14 strains that caused symptomatic infections, 12 strains were identified as GII.3[P12], and others were GII.4[P31]. Conversely, 17 asymptomatic infections were caused by GII.4[P31], two by GII.2[P16], and one by GII.4[P16]. Regardless of symptomatic and asymptomatic infections, the mutations were detected frequently in the ORF2 region, and almost all recombination were identified in the RdRp-ORF2 region. The majority of the mutations were located around the predefined epitope regions of P2 subdomain indicating a potential for immune evasion. Conclusion The role of symptomatic as well as asymptomatic infections in the evolution of norovirus needs to be evaluated continuously.https://doi.org/10.1186/s12985-023-02024-zNorovirusAcute gastroenteritisBirth cohortMutationRecombination |
| spellingShingle | Li-Na Chen Si-Jie Wang Song-Mei Wang Xiao-Li Fu Wen-Jing Zheng Zhi-Yong Hao Hai-Song Zhou Xin-Jiang Zhang Yu-Liang Zhao Chao Qiu Lorenz von Seidlein Tian-Yi Qiu Xuan-Yi Wang Molecular epidemiology analysis of symptomatic and asymptomatic norovirus infections in Chinese infants Virology Journal Norovirus Acute gastroenteritis Birth cohort Mutation Recombination |
| title | Molecular epidemiology analysis of symptomatic and asymptomatic norovirus infections in Chinese infants |
| title_full | Molecular epidemiology analysis of symptomatic and asymptomatic norovirus infections in Chinese infants |
| title_fullStr | Molecular epidemiology analysis of symptomatic and asymptomatic norovirus infections in Chinese infants |
| title_full_unstemmed | Molecular epidemiology analysis of symptomatic and asymptomatic norovirus infections in Chinese infants |
| title_short | Molecular epidemiology analysis of symptomatic and asymptomatic norovirus infections in Chinese infants |
| title_sort | molecular epidemiology analysis of symptomatic and asymptomatic norovirus infections in chinese infants |
| topic | Norovirus Acute gastroenteritis Birth cohort Mutation Recombination |
| url | https://doi.org/10.1186/s12985-023-02024-z |
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