SV2B/miR-34a/miR-128 axis as prognostic biomarker in glioblastoma multiforme
Abstract Glioblastoma (GBM) is a heterogenous primary brain tumour that is characterised with unfavourable patient prognosis. The identification of biomarkers for managing brain malignancies is of utmost importance. MicroRNAs (miRNAs) are small, non-coding RNAs implicated in cancer development. This...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-55917-6 |
| _version_ | 1827310258412847104 |
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| author | D. Mustafov S. S. Siddiqui L. Klena E. Karteris M. Braoudaki |
| author_facet | D. Mustafov S. S. Siddiqui L. Klena E. Karteris M. Braoudaki |
| author_sort | D. Mustafov |
| collection | DOAJ |
| description | Abstract Glioblastoma (GBM) is a heterogenous primary brain tumour that is characterised with unfavourable patient prognosis. The identification of biomarkers for managing brain malignancies is of utmost importance. MicroRNAs (miRNAs) are small, non-coding RNAs implicated in cancer development. This study aimed to assess the prognostic significance of miRNAs and their gene targets in GBM. An in silico approach was employed to investigate the differentially expressed miRNAs in GBM. The most dysregulated miRNAs were identified and analysed via Sfold in association with their gene target. The candidate gene was studied via multi-omics approaches, followed by in vitro and in vivo experiments. The in silico analyses revealed that miR-128a and miR-34a were significantly downregulated within GBM. Both miRNAs displayed high binding affinity to the synaptic vesicle glycoprotein 2B (SV2B) 3′ untranslated region (3′UTR). SV2B exhibited upregulation within brain regions with high synaptic activity. Significantly higher SV2B levels were observed in high grade brain malignancies in comparison to their normal counterparts. SV2B expression was observed across the cytoplasm of GBM cells. Our findings underscored the downregulated expression patterns of miR-128a and miR-34a, alongside the upregulation of SV2B in GBM suggesting the importance of the SV2B/miR-34a/miR-128 axis as a potential prognostic approach in GBM management. |
| first_indexed | 2024-04-24T19:56:50Z |
| format | Article |
| id | doaj.art-31bad21530514ae19760c2d7c1566b68 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T19:56:50Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-31bad21530514ae19760c2d7c1566b682024-03-24T12:16:08ZengNature PortfolioScientific Reports2045-23222024-03-0114111310.1038/s41598-024-55917-6SV2B/miR-34a/miR-128 axis as prognostic biomarker in glioblastoma multiformeD. Mustafov0S. S. Siddiqui1L. Klena2E. Karteris3M. Braoudaki4School of Life and Medical Sciences, University of HertfordshireSchool of Life and Medical Sciences, University of HertfordshireSchool of Life and Medical Sciences, University of HertfordshireCollege of Health, Medicine and Life Sciences, Brunel University LondonSchool of Life and Medical Sciences, University of HertfordshireAbstract Glioblastoma (GBM) is a heterogenous primary brain tumour that is characterised with unfavourable patient prognosis. The identification of biomarkers for managing brain malignancies is of utmost importance. MicroRNAs (miRNAs) are small, non-coding RNAs implicated in cancer development. This study aimed to assess the prognostic significance of miRNAs and their gene targets in GBM. An in silico approach was employed to investigate the differentially expressed miRNAs in GBM. The most dysregulated miRNAs were identified and analysed via Sfold in association with their gene target. The candidate gene was studied via multi-omics approaches, followed by in vitro and in vivo experiments. The in silico analyses revealed that miR-128a and miR-34a were significantly downregulated within GBM. Both miRNAs displayed high binding affinity to the synaptic vesicle glycoprotein 2B (SV2B) 3′ untranslated region (3′UTR). SV2B exhibited upregulation within brain regions with high synaptic activity. Significantly higher SV2B levels were observed in high grade brain malignancies in comparison to their normal counterparts. SV2B expression was observed across the cytoplasm of GBM cells. Our findings underscored the downregulated expression patterns of miR-128a and miR-34a, alongside the upregulation of SV2B in GBM suggesting the importance of the SV2B/miR-34a/miR-128 axis as a potential prognostic approach in GBM management.https://doi.org/10.1038/s41598-024-55917-6 |
| spellingShingle | D. Mustafov S. S. Siddiqui L. Klena E. Karteris M. Braoudaki SV2B/miR-34a/miR-128 axis as prognostic biomarker in glioblastoma multiforme Scientific Reports |
| title | SV2B/miR-34a/miR-128 axis as prognostic biomarker in glioblastoma multiforme |
| title_full | SV2B/miR-34a/miR-128 axis as prognostic biomarker in glioblastoma multiforme |
| title_fullStr | SV2B/miR-34a/miR-128 axis as prognostic biomarker in glioblastoma multiforme |
| title_full_unstemmed | SV2B/miR-34a/miR-128 axis as prognostic biomarker in glioblastoma multiforme |
| title_short | SV2B/miR-34a/miR-128 axis as prognostic biomarker in glioblastoma multiforme |
| title_sort | sv2b mir 34a mir 128 axis as prognostic biomarker in glioblastoma multiforme |
| url | https://doi.org/10.1038/s41598-024-55917-6 |
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