The anti-proliferative and anti-androgenic activity of different pomegranate accessions
This study monitored the anti-proliferative activity of pomegranate (Punica granatum L.) peel homogenates against breast (MCF7 and MDA-MB-453) and prostate (LNCaP and PC-3) cancer cell lines using 29 different accessions to study the natural diversity in these accessions. Using MTT method, it was re...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-10-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464616302389 |
| _version_ | 1831584710373933056 |
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| author | Ola Orgil Limor Spector Doron Holland Jamal Mahajna Rachel Amir |
| author_facet | Ola Orgil Limor Spector Doron Holland Jamal Mahajna Rachel Amir |
| author_sort | Ola Orgil |
| collection | DOAJ |
| description | This study monitored the anti-proliferative activity of pomegranate (Punica granatum L.) peel homogenates against breast (MCF7 and MDA-MB-453) and prostate (LNCaP and PC-3) cancer cell lines using 29 different accessions to study the natural diversity in these accessions. Using MTT method, it was revealed that high anti-proliferative activity was observed against MCF7, MDA-MB-453 and LNCaP (androgen-dependent) cell lines for most accessions, while the androgen-independent prostate cancer cell line, PC-3, exhibited relatively high resistance. Peel extracts displayed the highest LNCaP inhibition activity compared to aril juice and seed homogenates. Eight accessions exhibiting the highest anti-proliferative activity were studied further using the LNCaP and PC-3 cancer cells. IC50 and clonogenicity inhibition were determined. Several accessions were found to inhibit androgen receptor activity but not NF-κB transcription function. This study illustrated the importance of exploiting natural pomegranate variation to identify accessions exhibiting potential selective activity for the treatment of specific types of cancer. |
| first_indexed | 2024-12-17T21:04:09Z |
| format | Article |
| id | doaj.art-31bae075a2564ef688ec696b8b77c5a5 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T21:04:09Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-31bae075a2564ef688ec696b8b77c5a52022-12-21T21:32:38ZengElsevierJournal of Functional Foods1756-46462016-10-0126517528The anti-proliferative and anti-androgenic activity of different pomegranate accessionsOla Orgil0Limor Spector1Doron Holland2Jamal Mahajna3Rachel Amir4Migal Galilee Technology Center, P.O. Box 831, Kiryat Shmona 11016, Israel; Tel Hai College, Upper Galilee, IsraelMigal Galilee Technology Center, P.O. Box 831, Kiryat Shmona 11016, IsraelInstitute of Plant Sciences, Agricultural Research Organization, Newe Ya'ar Research Center, Ramat Yishay 30095, IsraelMigal Galilee Technology Center, P.O. Box 831, Kiryat Shmona 11016, Israel; Tel Hai College, Upper Galilee, IsraelMigal Galilee Technology Center, P.O. Box 831, Kiryat Shmona 11016, Israel; Tel Hai College, Upper Galilee, Israel; Corresponding author. Laboratory of Plant Science, Migal Galilee Technology Center, P.O. Box 831, Kiryat Shmona 11016, Israel. Fax: +972 4 6944980.This study monitored the anti-proliferative activity of pomegranate (Punica granatum L.) peel homogenates against breast (MCF7 and MDA-MB-453) and prostate (LNCaP and PC-3) cancer cell lines using 29 different accessions to study the natural diversity in these accessions. Using MTT method, it was revealed that high anti-proliferative activity was observed against MCF7, MDA-MB-453 and LNCaP (androgen-dependent) cell lines for most accessions, while the androgen-independent prostate cancer cell line, PC-3, exhibited relatively high resistance. Peel extracts displayed the highest LNCaP inhibition activity compared to aril juice and seed homogenates. Eight accessions exhibiting the highest anti-proliferative activity were studied further using the LNCaP and PC-3 cancer cells. IC50 and clonogenicity inhibition were determined. Several accessions were found to inhibit androgen receptor activity but not NF-κB transcription function. This study illustrated the importance of exploiting natural pomegranate variation to identify accessions exhibiting potential selective activity for the treatment of specific types of cancer.http://www.sciencedirect.com/science/article/pii/S1756464616302389Androgen receptorAnti-proliferative activityCancer cell linesDifferent accessionsPhenolic compoundsPomegranate |
| spellingShingle | Ola Orgil Limor Spector Doron Holland Jamal Mahajna Rachel Amir The anti-proliferative and anti-androgenic activity of different pomegranate accessions Journal of Functional Foods Androgen receptor Anti-proliferative activity Cancer cell lines Different accessions Phenolic compounds Pomegranate |
| title | The anti-proliferative and anti-androgenic activity of different pomegranate accessions |
| title_full | The anti-proliferative and anti-androgenic activity of different pomegranate accessions |
| title_fullStr | The anti-proliferative and anti-androgenic activity of different pomegranate accessions |
| title_full_unstemmed | The anti-proliferative and anti-androgenic activity of different pomegranate accessions |
| title_short | The anti-proliferative and anti-androgenic activity of different pomegranate accessions |
| title_sort | anti proliferative and anti androgenic activity of different pomegranate accessions |
| topic | Androgen receptor Anti-proliferative activity Cancer cell lines Different accessions Phenolic compounds Pomegranate |
| url | http://www.sciencedirect.com/science/article/pii/S1756464616302389 |
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