Simultaneous detection of α-Lactoalbumin, β-Lactoglobulin and Lactoferrin in milk by Visualized Microarray
Abstract Background α-Lactalbumin (a-LA), β-lactoglobulin (β-LG) and lactoferrin (LF) are of high nutritional value which have made ingredients of choice in the formulation of modern foods and beverages. There remains an urgent need to develop novel biosensing methods for quantification featuring re...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-09-01
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| Series: | BMC Biotechnology |
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| Online Access: | http://link.springer.com/article/10.1186/s12896-017-0387-9 |
| _version_ | 1818243417459654656 |
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| author | Zhoumin Li Fang Wen Zhonghui Li Nan Zheng Jindou Jiang Danke Xu |
| author_facet | Zhoumin Li Fang Wen Zhonghui Li Nan Zheng Jindou Jiang Danke Xu |
| author_sort | Zhoumin Li |
| collection | DOAJ |
| description | Abstract Background α-Lactalbumin (a-LA), β-lactoglobulin (β-LG) and lactoferrin (LF) are of high nutritional value which have made ingredients of choice in the formulation of modern foods and beverages. There remains an urgent need to develop novel biosensing methods for quantification featuring reduced cost, improved sensitivity, selectivity and more rapid response, especially for simultaneous detection of multiple whey proteins. Results A novel visualized microarray method was developed for the determination of a-LA, β-LG and LF in milk samples without the need for complex or time-consuming pre-treatment steps. The measurement principle was based on the competitive immunological reaction and silver enhancement technique. In this case, a visible array dots as the detectable signals were further amplified and developed by the silver enhancement reagents. The microarray could be assayed by the microarray scanner. The detection limits (S/N = 3) were estimated to be 40 ng/mL (α-LA), 50 ng/mL (β-LG), 30 ng/mL (LF) (n = 6). Conclusions The method could be used to simultaneously analyze the whey protein contents of various raw milk samples and ultra-high temperature treated (UHT) milk samples including skimmed milk and high calcium milk. The analytical results were in good agreement with that of the high performance liquid chromatography. The presented visualized microarray has showed its advantages such as high-throughput, specificity, sensitivity and cost-effective for analysis of various milk samples. |
| first_indexed | 2024-12-12T14:00:48Z |
| format | Article |
| id | doaj.art-31bae4e139e6426a961a17949e1dc75f |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-12-12T14:00:48Z |
| publishDate | 2017-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-31bae4e139e6426a961a17949e1dc75f2022-12-22T00:22:21ZengBMCBMC Biotechnology1472-67502017-09-011711910.1186/s12896-017-0387-9Simultaneous detection of α-Lactoalbumin, β-Lactoglobulin and Lactoferrin in milk by Visualized MicroarrayZhoumin Li0Fang Wen1Zhonghui Li2Nan Zheng3Jindou Jiang4Danke Xu5State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing UniversityMinistry of Agriculture-Key Laboratory of Quality & Safety Control for Milk and Dairy Products, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing UniversityMinistry of Agriculture-Key Laboratory of Quality & Safety Control for Milk and Dairy Products, Institute of Animal Science, Chinese Academy of Agricultural SciencesMinistry of Agriculture Dairy Quality Supervision and Testing CenterState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing UniversityAbstract Background α-Lactalbumin (a-LA), β-lactoglobulin (β-LG) and lactoferrin (LF) are of high nutritional value which have made ingredients of choice in the formulation of modern foods and beverages. There remains an urgent need to develop novel biosensing methods for quantification featuring reduced cost, improved sensitivity, selectivity and more rapid response, especially for simultaneous detection of multiple whey proteins. Results A novel visualized microarray method was developed for the determination of a-LA, β-LG and LF in milk samples without the need for complex or time-consuming pre-treatment steps. The measurement principle was based on the competitive immunological reaction and silver enhancement technique. In this case, a visible array dots as the detectable signals were further amplified and developed by the silver enhancement reagents. The microarray could be assayed by the microarray scanner. The detection limits (S/N = 3) were estimated to be 40 ng/mL (α-LA), 50 ng/mL (β-LG), 30 ng/mL (LF) (n = 6). Conclusions The method could be used to simultaneously analyze the whey protein contents of various raw milk samples and ultra-high temperature treated (UHT) milk samples including skimmed milk and high calcium milk. The analytical results were in good agreement with that of the high performance liquid chromatography. The presented visualized microarray has showed its advantages such as high-throughput, specificity, sensitivity and cost-effective for analysis of various milk samples.http://link.springer.com/article/10.1186/s12896-017-0387-9Visualized microarrayα-Lactoalbuminβ-LactoglobulinLactoferrin |
| spellingShingle | Zhoumin Li Fang Wen Zhonghui Li Nan Zheng Jindou Jiang Danke Xu Simultaneous detection of α-Lactoalbumin, β-Lactoglobulin and Lactoferrin in milk by Visualized Microarray BMC Biotechnology Visualized microarray α-Lactoalbumin β-Lactoglobulin Lactoferrin |
| title | Simultaneous detection of α-Lactoalbumin, β-Lactoglobulin and Lactoferrin in milk by Visualized Microarray |
| title_full | Simultaneous detection of α-Lactoalbumin, β-Lactoglobulin and Lactoferrin in milk by Visualized Microarray |
| title_fullStr | Simultaneous detection of α-Lactoalbumin, β-Lactoglobulin and Lactoferrin in milk by Visualized Microarray |
| title_full_unstemmed | Simultaneous detection of α-Lactoalbumin, β-Lactoglobulin and Lactoferrin in milk by Visualized Microarray |
| title_short | Simultaneous detection of α-Lactoalbumin, β-Lactoglobulin and Lactoferrin in milk by Visualized Microarray |
| title_sort | simultaneous detection of α lactoalbumin β lactoglobulin and lactoferrin in milk by visualized microarray |
| topic | Visualized microarray α-Lactoalbumin β-Lactoglobulin Lactoferrin |
| url | http://link.springer.com/article/10.1186/s12896-017-0387-9 |
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