Antidepressant Effects of Pharmacopuncture on Behavior and Brain-Derived Neurotrophic Factor (BDNF) Expression in Chronic Stress Model of Mice
Objectives: The present study aimed to investigate the antidepressant effect of the traditional Korean medical pharmacopuncture, Liver Qi Depression (HJ11), in a mouse model of depression induced by exposure to chronic immobilization stress (CIS). Methods: Mice were subjected to 2 hours of immobiliz...
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| Format: | Article |
| Language: | English |
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Medical Association of Pharmacopuncture Institute
2017-12-01
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| Series: | Journal of Acupuncture & Meridian Studies |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2005290117301139 |
| _version_ | 1818657536876740608 |
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| author | Yunna Kim Hwa-Young Lee Seung-Hun Cho |
| author_facet | Yunna Kim Hwa-Young Lee Seung-Hun Cho |
| author_sort | Yunna Kim |
| collection | DOAJ |
| description | Objectives: The present study aimed to investigate the antidepressant effect of the traditional Korean medical pharmacopuncture, Liver Qi Depression (HJ11), in a mouse model of depression induced by exposure to chronic immobilization stress (CIS).
Methods: Mice were subjected to 2 hours of immobilization stress daily for 14 days. They were also injected with distilled water (DW) (CIS + DW) or HJ11 at the acupoints HT7, SP6, and GV20 (CIS + HJ11) an hour before stress. The positive control group (CIS + paroxetine) was intraperitoneally injected with paroxetine (10 mg/kg, 14 days). The tail suspension test and the forced swimming test were performed to assess depression-like behaviors. Western blotting was also conducted to seek the change in brain.
Results: CIS + DW mice showed significantly longer immobile times in the tail suspension test and forced swimming test than sham mice that did not go through daily restraint. Immobility of CIS + HJ11 and that of CIS + paroxetine mice was significantly decreased compared with immobility of CIS + DW mice. Immunoblotting showed that HJ11 increased the expression of brain-derived neurotrophic factor both in the hippocampus and the amygdala.
Conclusion: HJ11 improves depressive-like behaviors in the stress-induced mouse model of depression, and the results indicate that the neuroprotective effect of HJ11, identified by brain-derived neurotrophic factor expression, may play a critical role in its antidepressant effect. |
| first_indexed | 2024-12-17T03:43:03Z |
| format | Article |
| id | doaj.art-31bea2c775594c9d8066bb0e52ede12d |
| institution | Directory Open Access Journal |
| issn | 2005-2901 |
| language | English |
| last_indexed | 2024-12-17T03:43:03Z |
| publishDate | 2017-12-01 |
| publisher | Medical Association of Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Acupuncture & Meridian Studies |
| spelling | doaj.art-31bea2c775594c9d8066bb0e52ede12d2022-12-21T22:04:57ZengMedical Association of Pharmacopuncture InstituteJournal of Acupuncture & Meridian Studies2005-29012017-12-0110640240810.1016/j.jams.2017.08.007Antidepressant Effects of Pharmacopuncture on Behavior and Brain-Derived Neurotrophic Factor (BDNF) Expression in Chronic Stress Model of MiceYunna Kim0Hwa-Young Lee1Seung-Hun Cho2Department of Clinical Korean Medicine, Graduate School, Kyung Hee University, Seoul, South KoreaCollege of Korean Medicine, Kyung Hee University, Seoul, South KoreaCollege of Korean Medicine, Kyung Hee University, Seoul, South KoreaObjectives: The present study aimed to investigate the antidepressant effect of the traditional Korean medical pharmacopuncture, Liver Qi Depression (HJ11), in a mouse model of depression induced by exposure to chronic immobilization stress (CIS). Methods: Mice were subjected to 2 hours of immobilization stress daily for 14 days. They were also injected with distilled water (DW) (CIS + DW) or HJ11 at the acupoints HT7, SP6, and GV20 (CIS + HJ11) an hour before stress. The positive control group (CIS + paroxetine) was intraperitoneally injected with paroxetine (10 mg/kg, 14 days). The tail suspension test and the forced swimming test were performed to assess depression-like behaviors. Western blotting was also conducted to seek the change in brain. Results: CIS + DW mice showed significantly longer immobile times in the tail suspension test and forced swimming test than sham mice that did not go through daily restraint. Immobility of CIS + HJ11 and that of CIS + paroxetine mice was significantly decreased compared with immobility of CIS + DW mice. Immunoblotting showed that HJ11 increased the expression of brain-derived neurotrophic factor both in the hippocampus and the amygdala. Conclusion: HJ11 improves depressive-like behaviors in the stress-induced mouse model of depression, and the results indicate that the neuroprotective effect of HJ11, identified by brain-derived neurotrophic factor expression, may play a critical role in its antidepressant effect.http://www.sciencedirect.com/science/article/pii/S2005290117301139amygdalabrain-derived neurotrophic factorchronic immobilization stressdepressionhippocampuspharmacopuncture |
| spellingShingle | Yunna Kim Hwa-Young Lee Seung-Hun Cho Antidepressant Effects of Pharmacopuncture on Behavior and Brain-Derived Neurotrophic Factor (BDNF) Expression in Chronic Stress Model of Mice Journal of Acupuncture & Meridian Studies amygdala brain-derived neurotrophic factor chronic immobilization stress depression hippocampus pharmacopuncture |
| title | Antidepressant Effects of Pharmacopuncture on Behavior and Brain-Derived Neurotrophic Factor (BDNF) Expression in Chronic Stress Model of Mice |
| title_full | Antidepressant Effects of Pharmacopuncture on Behavior and Brain-Derived Neurotrophic Factor (BDNF) Expression in Chronic Stress Model of Mice |
| title_fullStr | Antidepressant Effects of Pharmacopuncture on Behavior and Brain-Derived Neurotrophic Factor (BDNF) Expression in Chronic Stress Model of Mice |
| title_full_unstemmed | Antidepressant Effects of Pharmacopuncture on Behavior and Brain-Derived Neurotrophic Factor (BDNF) Expression in Chronic Stress Model of Mice |
| title_short | Antidepressant Effects of Pharmacopuncture on Behavior and Brain-Derived Neurotrophic Factor (BDNF) Expression in Chronic Stress Model of Mice |
| title_sort | antidepressant effects of pharmacopuncture on behavior and brain derived neurotrophic factor bdnf expression in chronic stress model of mice |
| topic | amygdala brain-derived neurotrophic factor chronic immobilization stress depression hippocampus pharmacopuncture |
| url | http://www.sciencedirect.com/science/article/pii/S2005290117301139 |
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