Implication of plant growth-promoting rhizobacteria of Bacillus spp. as biocontrol agents against wilt disease caused by Fusarium oxysporum Schlecht. in Vicia faba L.
Out of seven Fusarium spp. isolated from infected faba bean roots, two Fusarium oxysporum were selected and showed faba bean-wilt disease severity with percentages of 68% and 47% under greenhouse conditions. The F. oxysporum showed the highest wilt disease was selected to complete the current study....
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2021-12-01
|
| Series: | Biomolecular Concepts |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/bmc-2021-0020 |
| _version_ | 1818487884504629248 |
|---|---|
| author | El-Sersawy Mostafa Mohamed Hassan Saad El-Din El-Ghamry Abbas A. El-Gwad Amr Mahmoud Abd Fouda Amr |
| author_facet | El-Sersawy Mostafa Mohamed Hassan Saad El-Din El-Ghamry Abbas A. El-Gwad Amr Mahmoud Abd Fouda Amr |
| author_sort | El-Sersawy Mostafa Mohamed |
| collection | DOAJ |
| description | Out of seven Fusarium spp. isolated from infected faba bean roots, two Fusarium oxysporum were selected and showed faba bean-wilt disease severity with percentages of 68% and 47% under greenhouse conditions. The F. oxysporum showed the highest wilt disease was selected to complete the current study. Three rhizobacterial strains were isolated and identified as Bacillus velezensis Vb1, B. paramycoides Vb3, and B. paramycoides Vb6. These strains showed the highest in-vitro antagonistic activity by the dual-culture method against selected F. oxysporum with inhibition percentages of 59±0.2, 46±0.3, and 52±0.3% for Vb1, Vb3, and Vb6, respectively. These rhizobacterial strains exhibit varied activity for nitrogen-fixing and phosphate-solubilizing. Moreover, these strains showed positive results for ammonia, HCN, and siderophores production. The phytohormones production (indole-3-acetic acid, ABA, benzyl, kinten, ziaten, and GA3) and secretion of various lytic enzymes were recorded by these strains with varying degrees. Under greenhouse conditions, the rhizobacterial strains Vb1, Vb3, Vb6, and their consortium can protect faba bean from wilt caused by F. oxysporum with percentages of 70, 60, 65, and 82%, respectively. Under field conditions, the inoculation with the rhizobacterial consortium (Vb1+Vb3+Vb6) significantly increases the growth performance of the F. oxysporum-infected faba bean plant and recorded the highest wilt protection (83.3%). |
| first_indexed | 2024-12-10T16:44:06Z |
| format | Article |
| id | doaj.art-31bed3c50dd740178d1d23faef3b2307 |
| institution | Directory Open Access Journal |
| issn | 1868-5021 1868-503X |
| language | English |
| last_indexed | 2024-12-10T16:44:06Z |
| publishDate | 2021-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Biomolecular Concepts |
| spelling | doaj.art-31bed3c50dd740178d1d23faef3b23072022-12-22T01:41:07ZengDe GruyterBiomolecular Concepts1868-50211868-503X2021-12-0112119721410.1515/bmc-2021-0020Implication of plant growth-promoting rhizobacteria of Bacillus spp. as biocontrol agents against wilt disease caused by Fusarium oxysporum Schlecht. in Vicia faba L.El-Sersawy Mostafa Mohamed0Hassan Saad El-Din1El-Ghamry Abbas A.2El-Gwad Amr Mahmoud Abd3Fouda Amr4Soil Fertility and Microbiology Department, Desert Research Center,El-Mataria, Cairo, EgyptBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo11884, EgyptBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo11884, EgyptSoil Fertility and Microbiology Department, Desert Research Center,El-Mataria, Cairo, EgyptBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo11884, EgyptOut of seven Fusarium spp. isolated from infected faba bean roots, two Fusarium oxysporum were selected and showed faba bean-wilt disease severity with percentages of 68% and 47% under greenhouse conditions. The F. oxysporum showed the highest wilt disease was selected to complete the current study. Three rhizobacterial strains were isolated and identified as Bacillus velezensis Vb1, B. paramycoides Vb3, and B. paramycoides Vb6. These strains showed the highest in-vitro antagonistic activity by the dual-culture method against selected F. oxysporum with inhibition percentages of 59±0.2, 46±0.3, and 52±0.3% for Vb1, Vb3, and Vb6, respectively. These rhizobacterial strains exhibit varied activity for nitrogen-fixing and phosphate-solubilizing. Moreover, these strains showed positive results for ammonia, HCN, and siderophores production. The phytohormones production (indole-3-acetic acid, ABA, benzyl, kinten, ziaten, and GA3) and secretion of various lytic enzymes were recorded by these strains with varying degrees. Under greenhouse conditions, the rhizobacterial strains Vb1, Vb3, Vb6, and their consortium can protect faba bean from wilt caused by F. oxysporum with percentages of 70, 60, 65, and 82%, respectively. Under field conditions, the inoculation with the rhizobacterial consortium (Vb1+Vb3+Vb6) significantly increases the growth performance of the F. oxysporum-infected faba bean plant and recorded the highest wilt protection (83.3%).https://doi.org/10.1515/bmc-2021-0020fusarium wiltfusarium oxysporumplant growth-promotingvicia fabagreenhouse experimentfield experiment |
| spellingShingle | El-Sersawy Mostafa Mohamed Hassan Saad El-Din El-Ghamry Abbas A. El-Gwad Amr Mahmoud Abd Fouda Amr Implication of plant growth-promoting rhizobacteria of Bacillus spp. as biocontrol agents against wilt disease caused by Fusarium oxysporum Schlecht. in Vicia faba L. Biomolecular Concepts fusarium wilt fusarium oxysporum plant growth-promoting vicia faba greenhouse experiment field experiment |
| title | Implication of plant growth-promoting rhizobacteria of Bacillus spp. as biocontrol agents against wilt disease caused by Fusarium oxysporum Schlecht. in Vicia faba L. |
| title_full | Implication of plant growth-promoting rhizobacteria of Bacillus spp. as biocontrol agents against wilt disease caused by Fusarium oxysporum Schlecht. in Vicia faba L. |
| title_fullStr | Implication of plant growth-promoting rhizobacteria of Bacillus spp. as biocontrol agents against wilt disease caused by Fusarium oxysporum Schlecht. in Vicia faba L. |
| title_full_unstemmed | Implication of plant growth-promoting rhizobacteria of Bacillus spp. as biocontrol agents against wilt disease caused by Fusarium oxysporum Schlecht. in Vicia faba L. |
| title_short | Implication of plant growth-promoting rhizobacteria of Bacillus spp. as biocontrol agents against wilt disease caused by Fusarium oxysporum Schlecht. in Vicia faba L. |
| title_sort | implication of plant growth promoting rhizobacteria of bacillus spp as biocontrol agents against wilt disease caused by fusarium oxysporum schlecht in vicia faba l |
| topic | fusarium wilt fusarium oxysporum plant growth-promoting vicia faba greenhouse experiment field experiment |
| url | https://doi.org/10.1515/bmc-2021-0020 |
| work_keys_str_mv | AT elsersawymostafamohamed implicationofplantgrowthpromotingrhizobacteriaofbacillussppasbiocontrolagentsagainstwiltdiseasecausedbyfusariumoxysporumschlechtinviciafabal AT hassansaadeldin implicationofplantgrowthpromotingrhizobacteriaofbacillussppasbiocontrolagentsagainstwiltdiseasecausedbyfusariumoxysporumschlechtinviciafabal AT elghamryabbasa implicationofplantgrowthpromotingrhizobacteriaofbacillussppasbiocontrolagentsagainstwiltdiseasecausedbyfusariumoxysporumschlechtinviciafabal AT elgwadamrmahmoudabd implicationofplantgrowthpromotingrhizobacteriaofbacillussppasbiocontrolagentsagainstwiltdiseasecausedbyfusariumoxysporumschlechtinviciafabal AT foudaamr implicationofplantgrowthpromotingrhizobacteriaofbacillussppasbiocontrolagentsagainstwiltdiseasecausedbyfusariumoxysporumschlechtinviciafabal |