Genistein suppresses prostate cancer growth through inhibition of oncogenic microRNA-151.
Genistein has been shown to suppress the growth of several cancers through modulation of various pathways. However, the effects of genistein on the regulation of oncogenic microRNA-151 (miR-151) have not been reported. In this study, we investigated whether genistein could alter the expression of on...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3426544?pdf=render |
| _version_ | 1811199897626476544 |
|---|---|
| author | Takeshi Chiyomaru Soichiro Yamamura Mohd Saif Zaman Shahana Majid Guoren Deng Varahram Shahryari Sharanjot Saini Hiroshi Hirata Koji Ueno Inik Chang Yuichiro Tanaka Z Laura Tabatabai Hideki Enokida Masayuki Nakagawa Rajvir Dahiya |
| author_facet | Takeshi Chiyomaru Soichiro Yamamura Mohd Saif Zaman Shahana Majid Guoren Deng Varahram Shahryari Sharanjot Saini Hiroshi Hirata Koji Ueno Inik Chang Yuichiro Tanaka Z Laura Tabatabai Hideki Enokida Masayuki Nakagawa Rajvir Dahiya |
| author_sort | Takeshi Chiyomaru |
| collection | DOAJ |
| description | Genistein has been shown to suppress the growth of several cancers through modulation of various pathways. However, the effects of genistein on the regulation of oncogenic microRNA-151 (miR-151) have not been reported. In this study, we investigated whether genistein could alter the expression of oncogenic miR-151 and its target genes that are involved in the progression and metastasis of prostate cancer (PCa). Real-time RT-PCR showed that the expression of miR-151 was higher in PC3 and DU145 cells compared with RWPE-1 cells. Treatment of PC3 and DU145 cells with 25 µM genistein down-regulated the expression of miR-151 compared with vehicle control. Inhibition of miR-151 in PCa cells by genistein significantly inhibited cell migration and invasion. In-silico analysis showed that several genes (CASZ1, IL1RAPL1, SOX17, N4BP1 and ARHGDIA) suggested to have tumor suppressive functions were target genes of miR-151. Luciferase reporter assays indicated that miR-151 directly binds to specific sites on the 3'UTR of target genes. Quantitative real-time PCR analysis showed that the mRNA expression levels of the five target genes in PC3 and DU145 were markedly changed with miR-151 mimics and inhibitor. Kaplan-Meier curves and log-rank tests revealed that high expression levels of miR-151 had an adverse effect on survival rate. This study suggests that genistein mediated suppression of oncogenic miRNAs can be an important dietary therapeutic strategy for the treatment of PCa. |
| first_indexed | 2024-04-12T01:55:43Z |
| format | Article |
| id | doaj.art-31c0776254c1447fa50a3d5284da4cfe |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T01:55:43Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-31c0776254c1447fa50a3d5284da4cfe2022-12-22T03:52:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4381210.1371/journal.pone.0043812Genistein suppresses prostate cancer growth through inhibition of oncogenic microRNA-151.Takeshi ChiyomaruSoichiro YamamuraMohd Saif ZamanShahana MajidGuoren DengVarahram ShahryariSharanjot SainiHiroshi HirataKoji UenoInik ChangYuichiro TanakaZ Laura TabatabaiHideki EnokidaMasayuki NakagawaRajvir DahiyaGenistein has been shown to suppress the growth of several cancers through modulation of various pathways. However, the effects of genistein on the regulation of oncogenic microRNA-151 (miR-151) have not been reported. In this study, we investigated whether genistein could alter the expression of oncogenic miR-151 and its target genes that are involved in the progression and metastasis of prostate cancer (PCa). Real-time RT-PCR showed that the expression of miR-151 was higher in PC3 and DU145 cells compared with RWPE-1 cells. Treatment of PC3 and DU145 cells with 25 µM genistein down-regulated the expression of miR-151 compared with vehicle control. Inhibition of miR-151 in PCa cells by genistein significantly inhibited cell migration and invasion. In-silico analysis showed that several genes (CASZ1, IL1RAPL1, SOX17, N4BP1 and ARHGDIA) suggested to have tumor suppressive functions were target genes of miR-151. Luciferase reporter assays indicated that miR-151 directly binds to specific sites on the 3'UTR of target genes. Quantitative real-time PCR analysis showed that the mRNA expression levels of the five target genes in PC3 and DU145 were markedly changed with miR-151 mimics and inhibitor. Kaplan-Meier curves and log-rank tests revealed that high expression levels of miR-151 had an adverse effect on survival rate. This study suggests that genistein mediated suppression of oncogenic miRNAs can be an important dietary therapeutic strategy for the treatment of PCa.http://europepmc.org/articles/PMC3426544?pdf=render |
| spellingShingle | Takeshi Chiyomaru Soichiro Yamamura Mohd Saif Zaman Shahana Majid Guoren Deng Varahram Shahryari Sharanjot Saini Hiroshi Hirata Koji Ueno Inik Chang Yuichiro Tanaka Z Laura Tabatabai Hideki Enokida Masayuki Nakagawa Rajvir Dahiya Genistein suppresses prostate cancer growth through inhibition of oncogenic microRNA-151. PLoS ONE |
| title | Genistein suppresses prostate cancer growth through inhibition of oncogenic microRNA-151. |
| title_full | Genistein suppresses prostate cancer growth through inhibition of oncogenic microRNA-151. |
| title_fullStr | Genistein suppresses prostate cancer growth through inhibition of oncogenic microRNA-151. |
| title_full_unstemmed | Genistein suppresses prostate cancer growth through inhibition of oncogenic microRNA-151. |
| title_short | Genistein suppresses prostate cancer growth through inhibition of oncogenic microRNA-151. |
| title_sort | genistein suppresses prostate cancer growth through inhibition of oncogenic microrna 151 |
| url | http://europepmc.org/articles/PMC3426544?pdf=render |
| work_keys_str_mv | AT takeshichiyomaru genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 AT soichiroyamamura genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 AT mohdsaifzaman genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 AT shahanamajid genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 AT guorendeng genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 AT varahramshahryari genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 AT sharanjotsaini genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 AT hiroshihirata genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 AT kojiueno genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 AT inikchang genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 AT yuichirotanaka genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 AT zlauratabatabai genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 AT hidekienokida genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 AT masayukinakagawa genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 AT rajvirdahiya genisteinsuppressesprostatecancergrowththroughinhibitionofoncogenicmicrorna151 |