Genetic variability and identification of the intermediate snail hosts of Schistosoma mansoni
Studies based on shell or reproductive organ morphology and genetic considerations suggest extensive intraspecific variation in Biomphalaria snails. The high variability at the morphological and genetic levels, as well as the small size of some specimens and similarities between species complicate t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1998-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000700014 |
| _version_ | 1797718346036150272 |
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| author | Teofânia HDA Vidigal Emmanuel Dias Neto Linus Spatz Diana N Nunes Edina R Pires Andrew JG Simpson Omar S Carvalho |
| author_facet | Teofânia HDA Vidigal Emmanuel Dias Neto Linus Spatz Diana N Nunes Edina R Pires Andrew JG Simpson Omar S Carvalho |
| author_sort | Teofânia HDA Vidigal |
| collection | DOAJ |
| description | Studies based on shell or reproductive organ morphology and genetic considerations suggest extensive intraspecific variation in Biomphalaria snails. The high variability at the morphological and genetic levels, as well as the small size of some specimens and similarities between species complicate the correct identification of these snails. Here we review our work using methods based on polymerase chain reaction (PCR) amplification for analysis of genetic variation and identification of Biomphalaria snails from Brazil, Argentina, Uruguay and Paraguay. Arbitrarily primed-PCR revealed that the genome of B. glabrata exihibits a remarkable degree of intraespecific polymorphism. Low stringency-PCR using primers for 18S rRNA permited the identification of B. glabrata, B. tenagophila and B. occidentalis. The study of individuals obtained from geographically distinct populations exhibits significant intraspecific DNA polymorphism, however specimens from the same species, exhibit some species specific LSPs. We also showed that PCR-restriction fragment of length polymorphism of the internal transcribed spacer region of Biomphalaria rDNA, using DdeI permits the differentiation of the three intermediate hosts of Schistosoma mansoni. The molecular biological techniques used in our studies are very useful for the generation of new knowledge concerning the systematics and population genetics of Biomphalaria snails. |
| first_indexed | 2024-03-12T08:49:41Z |
| format | Article |
| id | doaj.art-31c5a3b2e9fc410fa6dbc017d386b477 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:49:41Z |
| publishDate | 1998-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-31c5a3b2e9fc410fa6dbc017d386b4772023-09-02T16:21:40ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601998-01-019310311010.1590/S0074-02761998000700014Genetic variability and identification of the intermediate snail hosts of Schistosoma mansoniTeofânia HDA VidigalEmmanuel Dias NetoLinus SpatzDiana N NunesEdina R PiresAndrew JG SimpsonOmar S CarvalhoStudies based on shell or reproductive organ morphology and genetic considerations suggest extensive intraspecific variation in Biomphalaria snails. The high variability at the morphological and genetic levels, as well as the small size of some specimens and similarities between species complicate the correct identification of these snails. Here we review our work using methods based on polymerase chain reaction (PCR) amplification for analysis of genetic variation and identification of Biomphalaria snails from Brazil, Argentina, Uruguay and Paraguay. Arbitrarily primed-PCR revealed that the genome of B. glabrata exihibits a remarkable degree of intraespecific polymorphism. Low stringency-PCR using primers for 18S rRNA permited the identification of B. glabrata, B. tenagophila and B. occidentalis. The study of individuals obtained from geographically distinct populations exhibits significant intraspecific DNA polymorphism, however specimens from the same species, exhibit some species specific LSPs. We also showed that PCR-restriction fragment of length polymorphism of the internal transcribed spacer region of Biomphalaria rDNA, using DdeI permits the differentiation of the three intermediate hosts of Schistosoma mansoni. The molecular biological techniques used in our studies are very useful for the generation of new knowledge concerning the systematics and population genetics of Biomphalaria snails.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000700014Biomphalariasnailsgenetic variabilityidentificationpolymerase chain reaction |
| spellingShingle | Teofânia HDA Vidigal Emmanuel Dias Neto Linus Spatz Diana N Nunes Edina R Pires Andrew JG Simpson Omar S Carvalho Genetic variability and identification of the intermediate snail hosts of Schistosoma mansoni Memorias do Instituto Oswaldo Cruz Biomphalaria snails genetic variability identification polymerase chain reaction |
| title | Genetic variability and identification of the intermediate snail hosts of Schistosoma mansoni |
| title_full | Genetic variability and identification of the intermediate snail hosts of Schistosoma mansoni |
| title_fullStr | Genetic variability and identification of the intermediate snail hosts of Schistosoma mansoni |
| title_full_unstemmed | Genetic variability and identification of the intermediate snail hosts of Schistosoma mansoni |
| title_short | Genetic variability and identification of the intermediate snail hosts of Schistosoma mansoni |
| title_sort | genetic variability and identification of the intermediate snail hosts of schistosoma mansoni |
| topic | Biomphalaria snails genetic variability identification polymerase chain reaction |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000700014 |
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