Isolation and Characterization of Listeria monocytogenes Phage vB_LmoH_P61, a Phage With Biocontrol Potential on Different Food Matrices
The high mortality rate associated with Listeria monocytogenes as well as its ability to adapt to the harsh conditions employed in food processing have ensured that this pathogen has become a significant concern in the ready-to-eat food industry. Lytic bacteriophages are viruses that hijack the meta...
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Sustainable Food Systems |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsufs.2020.521645/full |
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| author | Edel Stone Edel Stone Antoine Lhomet Antoine Lhomet Horst Neve Irene R. Grant Katrina Campbell Olivia McAuliffe Olivia McAuliffe |
| author_facet | Edel Stone Edel Stone Antoine Lhomet Antoine Lhomet Horst Neve Irene R. Grant Katrina Campbell Olivia McAuliffe Olivia McAuliffe |
| author_sort | Edel Stone |
| collection | DOAJ |
| description | The high mortality rate associated with Listeria monocytogenes as well as its ability to adapt to the harsh conditions employed in food processing have ensured that this pathogen has become a significant concern in the ready-to-eat food industry. Lytic bacteriophages are viruses that hijack the metabolic mechanisms of their bacterial host as a means to grow and replicate, subsequently leading to host cell death due to lysis. This study reports the biological and genomic characterization of L. monocytogenes phage vB_LmoH_P61 (P61) and its potential application for the reduction of L. monocytogenes in artificially contaminated foods. Phage P61 is a virulent bacteriophage belonging to the family Herelleviridae and has a genome size of 136,485bp. The lytic spectrum of phage P61 was investigated and it was shown to infect serotypes 1/2a, 1/2b, 1/2c, 4b, 4e, and 6a. Treatment of artificially contaminated milk stored at 8 and 12°C with phage P61 resulted in a significant reduction in L. monocytogenes numbers over the product shelf life. Similarly, phage P61 reduced L. monocytogenes numbers on artificially contaminated baby spinach stored at 8, 12, and 25°C. The research findings indicate that biocontrol of L. monocytogenes with phage P61 may offer a safe and environmentally friendly approach for the reduction of L. monocytogenes numbers in certain ready-to-eat foods. |
| first_indexed | 2024-12-11T19:04:09Z |
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| id | doaj.art-31c65ecf98e145df801f3a329946e296 |
| institution | Directory Open Access Journal |
| issn | 2571-581X |
| language | English |
| last_indexed | 2024-12-11T19:04:09Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sustainable Food Systems |
| spelling | doaj.art-31c65ecf98e145df801f3a329946e2962022-12-22T00:53:57ZengFrontiers Media S.A.Frontiers in Sustainable Food Systems2571-581X2020-11-01410.3389/fsufs.2020.521645521645Isolation and Characterization of Listeria monocytogenes Phage vB_LmoH_P61, a Phage With Biocontrol Potential on Different Food MatricesEdel Stone0Edel Stone1Antoine Lhomet2Antoine Lhomet3Horst Neve4Irene R. Grant5Katrina Campbell6Olivia McAuliffe7Olivia McAuliffe8Teagasc Food Research Centre, Moorepark, Cork, IrelandInstitute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, United KingdomTeagasc Food Research Centre, Moorepark, Cork, IrelandIUT Dijon-Auxerre, University of Burgundy, Dijon, FranceDepartment of Microbiology and Biotechnology, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Kiel, GermanyInstitute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, United KingdomInstitute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, United KingdomTeagasc Food Research Centre, Moorepark, Cork, IrelandVistaMilk SFI Research Centre, Cork, IrelandThe high mortality rate associated with Listeria monocytogenes as well as its ability to adapt to the harsh conditions employed in food processing have ensured that this pathogen has become a significant concern in the ready-to-eat food industry. Lytic bacteriophages are viruses that hijack the metabolic mechanisms of their bacterial host as a means to grow and replicate, subsequently leading to host cell death due to lysis. This study reports the biological and genomic characterization of L. monocytogenes phage vB_LmoH_P61 (P61) and its potential application for the reduction of L. monocytogenes in artificially contaminated foods. Phage P61 is a virulent bacteriophage belonging to the family Herelleviridae and has a genome size of 136,485bp. The lytic spectrum of phage P61 was investigated and it was shown to infect serotypes 1/2a, 1/2b, 1/2c, 4b, 4e, and 6a. Treatment of artificially contaminated milk stored at 8 and 12°C with phage P61 resulted in a significant reduction in L. monocytogenes numbers over the product shelf life. Similarly, phage P61 reduced L. monocytogenes numbers on artificially contaminated baby spinach stored at 8, 12, and 25°C. The research findings indicate that biocontrol of L. monocytogenes with phage P61 may offer a safe and environmentally friendly approach for the reduction of L. monocytogenes numbers in certain ready-to-eat foods.https://www.frontiersin.org/articles/10.3389/fsufs.2020.521645/fullbacteriophageListeria monocytogenesbiocontrolbroad-host rangefood safety |
| spellingShingle | Edel Stone Edel Stone Antoine Lhomet Antoine Lhomet Horst Neve Irene R. Grant Katrina Campbell Olivia McAuliffe Olivia McAuliffe Isolation and Characterization of Listeria monocytogenes Phage vB_LmoH_P61, a Phage With Biocontrol Potential on Different Food Matrices Frontiers in Sustainable Food Systems bacteriophage Listeria monocytogenes biocontrol broad-host range food safety |
| title | Isolation and Characterization of Listeria monocytogenes Phage vB_LmoH_P61, a Phage With Biocontrol Potential on Different Food Matrices |
| title_full | Isolation and Characterization of Listeria monocytogenes Phage vB_LmoH_P61, a Phage With Biocontrol Potential on Different Food Matrices |
| title_fullStr | Isolation and Characterization of Listeria monocytogenes Phage vB_LmoH_P61, a Phage With Biocontrol Potential on Different Food Matrices |
| title_full_unstemmed | Isolation and Characterization of Listeria monocytogenes Phage vB_LmoH_P61, a Phage With Biocontrol Potential on Different Food Matrices |
| title_short | Isolation and Characterization of Listeria monocytogenes Phage vB_LmoH_P61, a Phage With Biocontrol Potential on Different Food Matrices |
| title_sort | isolation and characterization of listeria monocytogenes phage vb lmoh p61 a phage with biocontrol potential on different food matrices |
| topic | bacteriophage Listeria monocytogenes biocontrol broad-host range food safety |
| url | https://www.frontiersin.org/articles/10.3389/fsufs.2020.521645/full |
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