In Vivo Evaluation of the Effects of B-Doped Strontium Apatite Nanoparticles Produced by Hydrothermal Method on Bone Repair
In the present study, the structural, morphological, and in vivo biocompatibility of un-doped and boron (B)-doped strontium apatite (SrAp) nanoparticles were investigated. Biomaterials were fabricated using the hydrothermal process. The structural and morphological characterizations of the fabricate...
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MDPI AG
2022-07-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/13/3/110 |
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| author | Faruk Oztekin Turan Gurgenc Serkan Dundar Ibrahim Hanifi Ozercan Tuba Talo Yildirim Mehmet Eskibaglar Erhan Cahit Ozcan Cevher Kursat Macit |
| author_facet | Faruk Oztekin Turan Gurgenc Serkan Dundar Ibrahim Hanifi Ozercan Tuba Talo Yildirim Mehmet Eskibaglar Erhan Cahit Ozcan Cevher Kursat Macit |
| author_sort | Faruk Oztekin |
| collection | DOAJ |
| description | In the present study, the structural, morphological, and in vivo biocompatibility of un-doped and boron (B)-doped strontium apatite (SrAp) nanoparticles were investigated. Biomaterials were fabricated using the hydrothermal process. The structural and morphological characterizations of the fabricated nanoparticles were performed by XRD, FT-IR, FE-SEM, and EDX. Their biocompatibility was investigated by placing them in defects in rat tibiae in vivo. The un-doped and B-doped SrAp nanoparticles were successfully fabricated. The produced nanoparticles were in the shape of nano-rods, and the dimensions of the nano-rods decreased as the B ratio increased. It was observed that the structural and morphological properties of strontium apatite nanoparticles were affected by the contribution of B. A stoichiometric Sr/P ratio of 1.67 was reached in the 5% B-doped sample (1.68). The average crystallite sizes were 34.94 nm, 39.70 nm, 44.93 nm, and 48.23 nm in un-doped, 1% B-doped, 5% B-doped, and 10% B-doped samples, respectively. The results of the in vivo experiment revealed that the new bone formation and osteoblast density were higher in the groups with SrAp nanoparticles doped with different concentrations of B than in the control group, in which the open defects were untreated. It was observed that this biocompatibility and the new bone formation were especially elevated in the B groups, which added high levels of strontium were added. The osteoblast density was higher in the group in which the strontium element was placed in the opened bone defect compared with the control group. However, although new bone formation was slightly higher in the strontium group than in the control group, the difference was not statistically significant. Furthermore, the strontium group had the highest amount of fibrotic tissue formation. The produced nanoparticles can be used in dental and orthopedic applications as biomaterials. |
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| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-09T23:34:53Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
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| series | Journal of Functional Biomaterials |
| spelling | doaj.art-31c70558453049da8dca84fb362aa5b32023-11-23T17:03:13ZengMDPI AGJournal of Functional Biomaterials2079-49832022-07-0113311010.3390/jfb13030110In Vivo Evaluation of the Effects of B-Doped Strontium Apatite Nanoparticles Produced by Hydrothermal Method on Bone RepairFaruk Oztekin0Turan Gurgenc1Serkan Dundar2Ibrahim Hanifi Ozercan3Tuba Talo Yildirim4Mehmet Eskibaglar5Erhan Cahit Ozcan6Cevher Kursat Macit7Department of Endodontics, Faculty of Dentistry, Firat University, Elazig 23100, TurkeyFaculty of Technology, Firat University, Elazig 23100, TurkeyDepartment of Periodontology, Faculty of Dentistry, Firat University, Elazig 23100, TurkeyDepartment of Pathology, Faculty of Medicine, Firat University, Elazig 23100, TurkeyDepartment of Periodontology, Faculty of Dentistry, Firat University, Elazig 23100, TurkeyDepartment of Endodontics, Faculty of Dentistry, Firat University, Elazig 23100, TurkeyDepartment of Esthetic, Plastic and Reconstructive Surgery, Faculty of Medicine, Firat University, Elazig 23100, TurkeyFaculty of Engineering, Firat University, Elazig 23100, TurkeyIn the present study, the structural, morphological, and in vivo biocompatibility of un-doped and boron (B)-doped strontium apatite (SrAp) nanoparticles were investigated. Biomaterials were fabricated using the hydrothermal process. The structural and morphological characterizations of the fabricated nanoparticles were performed by XRD, FT-IR, FE-SEM, and EDX. Their biocompatibility was investigated by placing them in defects in rat tibiae in vivo. The un-doped and B-doped SrAp nanoparticles were successfully fabricated. The produced nanoparticles were in the shape of nano-rods, and the dimensions of the nano-rods decreased as the B ratio increased. It was observed that the structural and morphological properties of strontium apatite nanoparticles were affected by the contribution of B. A stoichiometric Sr/P ratio of 1.67 was reached in the 5% B-doped sample (1.68). The average crystallite sizes were 34.94 nm, 39.70 nm, 44.93 nm, and 48.23 nm in un-doped, 1% B-doped, 5% B-doped, and 10% B-doped samples, respectively. The results of the in vivo experiment revealed that the new bone formation and osteoblast density were higher in the groups with SrAp nanoparticles doped with different concentrations of B than in the control group, in which the open defects were untreated. It was observed that this biocompatibility and the new bone formation were especially elevated in the B groups, which added high levels of strontium were added. The osteoblast density was higher in the group in which the strontium element was placed in the opened bone defect compared with the control group. However, although new bone formation was slightly higher in the strontium group than in the control group, the difference was not statistically significant. Furthermore, the strontium group had the highest amount of fibrotic tissue formation. The produced nanoparticles can be used in dental and orthopedic applications as biomaterials.https://www.mdpi.com/2079-4983/13/3/110endodontic surgerybone repairosteoblaststrontium apatitenanomaterial |
| spellingShingle | Faruk Oztekin Turan Gurgenc Serkan Dundar Ibrahim Hanifi Ozercan Tuba Talo Yildirim Mehmet Eskibaglar Erhan Cahit Ozcan Cevher Kursat Macit In Vivo Evaluation of the Effects of B-Doped Strontium Apatite Nanoparticles Produced by Hydrothermal Method on Bone Repair Journal of Functional Biomaterials endodontic surgery bone repair osteoblast strontium apatite nanomaterial |
| title | In Vivo Evaluation of the Effects of B-Doped Strontium Apatite Nanoparticles Produced by Hydrothermal Method on Bone Repair |
| title_full | In Vivo Evaluation of the Effects of B-Doped Strontium Apatite Nanoparticles Produced by Hydrothermal Method on Bone Repair |
| title_fullStr | In Vivo Evaluation of the Effects of B-Doped Strontium Apatite Nanoparticles Produced by Hydrothermal Method on Bone Repair |
| title_full_unstemmed | In Vivo Evaluation of the Effects of B-Doped Strontium Apatite Nanoparticles Produced by Hydrothermal Method on Bone Repair |
| title_short | In Vivo Evaluation of the Effects of B-Doped Strontium Apatite Nanoparticles Produced by Hydrothermal Method on Bone Repair |
| title_sort | in vivo evaluation of the effects of b doped strontium apatite nanoparticles produced by hydrothermal method on bone repair |
| topic | endodontic surgery bone repair osteoblast strontium apatite nanomaterial |
| url | https://www.mdpi.com/2079-4983/13/3/110 |
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