Methanol soluble fraction of fruits of Annona muricata possesses significant antidiarrheal activities
Medicinal plants are the major sources of traditional treatment of disease in Indian subcontinent due to abundant presence of plants and vast side effects of synthetic drug. The present study was subjected to observe in vitro thrombolytic, antibacterial, and in vivo antidiarrheal activities of metha...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844019367714 |
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| author | Nahida Afroz Md. Ahsanul Hoq Sharmin Jahan Md. Mainul Islam Firoz Ahmed A.F.M. Shahid-Ud-Daula Md. Hasanuzzaman |
| author_facet | Nahida Afroz Md. Ahsanul Hoq Sharmin Jahan Md. Mainul Islam Firoz Ahmed A.F.M. Shahid-Ud-Daula Md. Hasanuzzaman |
| author_sort | Nahida Afroz |
| collection | DOAJ |
| description | Medicinal plants are the major sources of traditional treatment of disease in Indian subcontinent due to abundant presence of plants and vast side effects of synthetic drug. The present study was subjected to observe in vitro thrombolytic, antibacterial, and in vivo antidiarrheal activities of methanol soluble fraction of fruits of Annona muricata. In thrombolytic activity assay, various concentrations (2 ─ 10 mg/ml) of methanol soluble fraction was used and dose dependently less potent activity was found. The maximum clot lysis 18.33% (p* < 0.05) was achieved at 10 mg/ml of methanolic fruit extract, whereas standard drug streptokinase showed 55.50% (p*** < 0.001) clot lysis. In antibacterial assay, disc diffusion method was used comprising two gram positive (S. aureus and Micrococcus luteus) and two gram negative (E. coli and P. aeruginosa) bacteria. None of four (0.25, 0.5, 1, and 5 mg/disc) concentration of fruit extract showed antibacterial potentiality, whereas standard amikacin (3 mg/disc) revealed strong antibacterial activities (=~ 23 ─ 24 mm of MIC). To evaluate antidiarrheal activity, castor oil induced diarrhea was created in Swiss albino mice and different doses (100, 200, and 400 mg/kgbw) of fruit extract was introduced post orally. All of three different doses of fruit extract showed significant (p < 0.05 ─ 0.001) antidiarrheal activities. Notably, the percent inhibition of diarrhea by methanolic extract of fruits of Annona muricata was found to be 58.38% at a dose of 400 mg/kgbw. The effect of vehicle saline (10 ml/kgbw) was considered as control and loperamide (5 mg/kgbw) as standard that provided 67.01% inhibition of diarrhea. The results suggest that, the fruits of Annona muricata possess potent antidiarrheal properties, providing scientific basis of using the plant parts in the treatment of diarrheal disease. |
| first_indexed | 2024-12-13T12:37:12Z |
| format | Article |
| id | doaj.art-31c9296c32284b61a126347e198d36fc |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-13T12:37:12Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj.art-31c9296c32284b61a126347e198d36fc2022-12-21T23:45:48ZengElsevierHeliyon2405-84402020-01-0161e03112Methanol soluble fraction of fruits of Annona muricata possesses significant antidiarrheal activitiesNahida Afroz0Md. Ahsanul Hoq1Sharmin Jahan2Md. Mainul Islam3Firoz Ahmed4A.F.M. Shahid-Ud-Daula5Md. Hasanuzzaman6Department of Pharmacy, Faculty of Science, Noakhali Science and Technology University, Noakhali, 3814, BangladeshDepartment of Pharmacy, Faculty of Science, Noakhali Science and Technology University, Noakhali, 3814, BangladeshDepartment of Pharmacy, Faculty of Science, Noakhali Science and Technology University, Noakhali, 3814, BangladeshDepartment of Pharmacy, Faculty of Science, Noakhali Science and Technology University, Noakhali, 3814, BangladeshDepartment of Microbiology, Faculty of Science, Noakhali Science and Technology University, Noakhali, 3814, BangladeshDepartment of Pharmacy, Faculty of Science, Noakhali Science and Technology University, Noakhali, 3814, BangladeshDepartment of Pharmacy, Faculty of Science, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh; Corresponding author.Medicinal plants are the major sources of traditional treatment of disease in Indian subcontinent due to abundant presence of plants and vast side effects of synthetic drug. The present study was subjected to observe in vitro thrombolytic, antibacterial, and in vivo antidiarrheal activities of methanol soluble fraction of fruits of Annona muricata. In thrombolytic activity assay, various concentrations (2 ─ 10 mg/ml) of methanol soluble fraction was used and dose dependently less potent activity was found. The maximum clot lysis 18.33% (p* < 0.05) was achieved at 10 mg/ml of methanolic fruit extract, whereas standard drug streptokinase showed 55.50% (p*** < 0.001) clot lysis. In antibacterial assay, disc diffusion method was used comprising two gram positive (S. aureus and Micrococcus luteus) and two gram negative (E. coli and P. aeruginosa) bacteria. None of four (0.25, 0.5, 1, and 5 mg/disc) concentration of fruit extract showed antibacterial potentiality, whereas standard amikacin (3 mg/disc) revealed strong antibacterial activities (=~ 23 ─ 24 mm of MIC). To evaluate antidiarrheal activity, castor oil induced diarrhea was created in Swiss albino mice and different doses (100, 200, and 400 mg/kgbw) of fruit extract was introduced post orally. All of three different doses of fruit extract showed significant (p < 0.05 ─ 0.001) antidiarrheal activities. Notably, the percent inhibition of diarrhea by methanolic extract of fruits of Annona muricata was found to be 58.38% at a dose of 400 mg/kgbw. The effect of vehicle saline (10 ml/kgbw) was considered as control and loperamide (5 mg/kgbw) as standard that provided 67.01% inhibition of diarrhea. The results suggest that, the fruits of Annona muricata possess potent antidiarrheal properties, providing scientific basis of using the plant parts in the treatment of diarrheal disease.http://www.sciencedirect.com/science/article/pii/S2405844019367714BiochemistryThrombolyticAnnona muricataAntidiarrhealMethanol |
| spellingShingle | Nahida Afroz Md. Ahsanul Hoq Sharmin Jahan Md. Mainul Islam Firoz Ahmed A.F.M. Shahid-Ud-Daula Md. Hasanuzzaman Methanol soluble fraction of fruits of Annona muricata possesses significant antidiarrheal activities Heliyon Biochemistry Thrombolytic Annona muricata Antidiarrheal Methanol |
| title | Methanol soluble fraction of fruits of Annona muricata possesses significant antidiarrheal activities |
| title_full | Methanol soluble fraction of fruits of Annona muricata possesses significant antidiarrheal activities |
| title_fullStr | Methanol soluble fraction of fruits of Annona muricata possesses significant antidiarrheal activities |
| title_full_unstemmed | Methanol soluble fraction of fruits of Annona muricata possesses significant antidiarrheal activities |
| title_short | Methanol soluble fraction of fruits of Annona muricata possesses significant antidiarrheal activities |
| title_sort | methanol soluble fraction of fruits of annona muricata possesses significant antidiarrheal activities |
| topic | Biochemistry Thrombolytic Annona muricata Antidiarrheal Methanol |
| url | http://www.sciencedirect.com/science/article/pii/S2405844019367714 |
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