Neuroprotective Activities of <i>Crossyne flava</i> Bulbs and Amaryllidaceae Alkaloids: Implications for Parkinson’s Disease
Parkinson’s disease (PD) is one of the most common neurodegenerative diseases and affects approximately 6.3 million people worldwide. To date, the treatment of PD remains a challenge, as available treatment options are known to be associated with serious side effects; hence, the search for new treat...
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MDPI AG
2021-06-01
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| Series: | Molecules |
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| author | Sylvester I. Omoruyi Abobaker S. Ibrakaw Okobi E. Ekpo James S. Boatwright Christopher N. Cupido Ahmed A. Hussein |
| author_facet | Sylvester I. Omoruyi Abobaker S. Ibrakaw Okobi E. Ekpo James S. Boatwright Christopher N. Cupido Ahmed A. Hussein |
| author_sort | Sylvester I. Omoruyi |
| collection | DOAJ |
| description | Parkinson’s disease (PD) is one of the most common neurodegenerative diseases and affects approximately 6.3 million people worldwide. To date, the treatment of PD remains a challenge, as available treatment options are known to be associated with serious side effects; hence, the search for new treatment strategies is critical. Extracts from the Amaryllidaceae plant family as well as their alkaloids have been reported to have neuroprotective potentials. This study, therefore, investigated the biological activities of <i>Crossyne flava</i> and its isolated alkaloids in an in vitro MPP<sup>+</sup> (1-methyl-4-phenylpyridinium) PD model using SH-SY5Y cells. The effects of the total extract as well as the four compounds isolated from <i>Crossyne flava</i> (i.e., pancratinine B (<b>1</b>), bufanidrine (<b>2</b>), buphanisine (<b>3</b>), and epibuphanisine (<b>4</b>)) were evaluated for cell viability, neuroprotection, levels of reactive oxygen species (ROS), adenosine triphosphate activity (ATP), and caspase 3/7 activity in SH-SY5Y cells. The results obtained showed that pre-treatment with both the extract and the isolated compounds was effective in protecting the SH-SY5Y cells from MPP<sup>+</sup>-induced neurotoxicity and inhibited ROS generation, ATP depletion as well as apoptosis induction in the SH-SY5Y cells. The results of this study show that the Amaryllidaceae plant family may be a source of novel compounds for the treatment of neurodegenerative diseases, which validates the reported traditional uses. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T09:56:32Z |
| publishDate | 2021-06-01 |
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| series | Molecules |
| spelling | doaj.art-31ca562402d84e5d8d17b94b2724fed02023-11-22T02:19:01ZengMDPI AGMolecules1420-30492021-06-012613399010.3390/molecules26133990Neuroprotective Activities of <i>Crossyne flava</i> Bulbs and Amaryllidaceae Alkaloids: Implications for Parkinson’s DiseaseSylvester I. Omoruyi0Abobaker S. Ibrakaw1Okobi E. Ekpo2James S. Boatwright3Christopher N. Cupido4Ahmed A. Hussein5Department of Chemistry, Cape Peninsula University of Technology, Symphony Road, Bellville 7535, South AfricaDepartment of Biodiversity and Conservation Biology, University of the Western Cape, Cape Town, Robert Sobukwe Road, Bellville 7535, South AfricaDepartment of Anatomy and Cellular Biology, College of Medicine and Health Sciences, Khalifa University, Abu Dhabi P.O. Box 127788, United Arab EmiratesDepartment of Biodiversity and Conservation Biology, University of the Western Cape, Cape Town, Robert Sobukwe Road, Bellville 7535, South AfricaDepartment of Botany, University of Fort Hare, Private Bag X1314, Alice 5700, South AfricaDepartment of Chemistry, Cape Peninsula University of Technology, Symphony Road, Bellville 7535, South AfricaParkinson’s disease (PD) is one of the most common neurodegenerative diseases and affects approximately 6.3 million people worldwide. To date, the treatment of PD remains a challenge, as available treatment options are known to be associated with serious side effects; hence, the search for new treatment strategies is critical. Extracts from the Amaryllidaceae plant family as well as their alkaloids have been reported to have neuroprotective potentials. This study, therefore, investigated the biological activities of <i>Crossyne flava</i> and its isolated alkaloids in an in vitro MPP<sup>+</sup> (1-methyl-4-phenylpyridinium) PD model using SH-SY5Y cells. The effects of the total extract as well as the four compounds isolated from <i>Crossyne flava</i> (i.e., pancratinine B (<b>1</b>), bufanidrine (<b>2</b>), buphanisine (<b>3</b>), and epibuphanisine (<b>4</b>)) were evaluated for cell viability, neuroprotection, levels of reactive oxygen species (ROS), adenosine triphosphate activity (ATP), and caspase 3/7 activity in SH-SY5Y cells. The results obtained showed that pre-treatment with both the extract and the isolated compounds was effective in protecting the SH-SY5Y cells from MPP<sup>+</sup>-induced neurotoxicity and inhibited ROS generation, ATP depletion as well as apoptosis induction in the SH-SY5Y cells. The results of this study show that the Amaryllidaceae plant family may be a source of novel compounds for the treatment of neurodegenerative diseases, which validates the reported traditional uses.https://www.mdpi.com/1420-3049/26/13/3990Amaryllidaceaealkaloids<i>Crossyne flava</i>Parkinson’s disease1-methyl-4-phenylpyridinium (MPP<sup>+</sup>)neuroprotection |
| spellingShingle | Sylvester I. Omoruyi Abobaker S. Ibrakaw Okobi E. Ekpo James S. Boatwright Christopher N. Cupido Ahmed A. Hussein Neuroprotective Activities of <i>Crossyne flava</i> Bulbs and Amaryllidaceae Alkaloids: Implications for Parkinson’s Disease Molecules Amaryllidaceae alkaloids <i>Crossyne flava</i> Parkinson’s disease 1-methyl-4-phenylpyridinium (MPP<sup>+</sup>) neuroprotection |
| title | Neuroprotective Activities of <i>Crossyne flava</i> Bulbs and Amaryllidaceae Alkaloids: Implications for Parkinson’s Disease |
| title_full | Neuroprotective Activities of <i>Crossyne flava</i> Bulbs and Amaryllidaceae Alkaloids: Implications for Parkinson’s Disease |
| title_fullStr | Neuroprotective Activities of <i>Crossyne flava</i> Bulbs and Amaryllidaceae Alkaloids: Implications for Parkinson’s Disease |
| title_full_unstemmed | Neuroprotective Activities of <i>Crossyne flava</i> Bulbs and Amaryllidaceae Alkaloids: Implications for Parkinson’s Disease |
| title_short | Neuroprotective Activities of <i>Crossyne flava</i> Bulbs and Amaryllidaceae Alkaloids: Implications for Parkinson’s Disease |
| title_sort | neuroprotective activities of i crossyne flava i bulbs and amaryllidaceae alkaloids implications for parkinson s disease |
| topic | Amaryllidaceae alkaloids <i>Crossyne flava</i> Parkinson’s disease 1-methyl-4-phenylpyridinium (MPP<sup>+</sup>) neuroprotection |
| url | https://www.mdpi.com/1420-3049/26/13/3990 |
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