Polystyrene nanoplastics promote the apoptosis in Caco-2 cells induced by okadaic acid more than microplastics
Microplastics (MPs) are widespread in the environment and can be ingested through food, water, and air, posing a threat to human health. In addition, MPs can have a potential combined effect with other toxic compounds. Polystyrene (PS) has been shown to enhance the cytotoxicity of okadaic acid (OA)....
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322012155 |
| _version_ | 1797961263889776640 |
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| author | Linhong Yan Zihua Yu Peichun Lin Shijie Qiu Liuying He Zijie Wu Lihua Ma Yanggao Gu Lei He Zhenqing Dai Chunxia Zhou Pengzhi Hong Chengyong Li |
| author_facet | Linhong Yan Zihua Yu Peichun Lin Shijie Qiu Liuying He Zijie Wu Lihua Ma Yanggao Gu Lei He Zhenqing Dai Chunxia Zhou Pengzhi Hong Chengyong Li |
| author_sort | Linhong Yan |
| collection | DOAJ |
| description | Microplastics (MPs) are widespread in the environment and can be ingested through food, water, and air, posing a threat to human health. In addition, MPs can have a potential combined effect with other toxic compounds. Polystyrene (PS) has been shown to enhance the cytotoxicity of okadaic acid (OA). However, it remains unclear whether this enhancement effect is related to the size of PS particles. In this study, we investigated the mechanism of the combined effect of PS microplastics (PS-MPs) or PS nanoplastics (PS-NPs) and OA on Caco-2 cells. The results indicated that PS-NPs enhanced the cytotoxicity of OA and induced endoplasmic reticulum (ER) stress-mediated apoptosis in Caco-2 cells, compared to PS-MPs. Specifically, PS-NPs and OA cause more severe oxidative stress, lactate dehydrogenase (LDH) release, and mitochondrial membrane depolarization. Furthermore, it induced intracellular calcium overload through store-operated channels (SOCs) and activated the PERK/ATF-4/CHOP pathway to cause ER stress. ER stress promoted mitochondrial damage and finally activated the caspase family to induce apoptosis. This study provided an indirect basis for the assessment of the combined toxicity of MPs or NPs with OA. |
| first_indexed | 2024-04-11T00:56:17Z |
| format | Article |
| id | doaj.art-31cb85e9e29d459881f6cf17e443d008 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-11T00:56:17Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-31cb85e9e29d459881f6cf17e443d0082023-01-05T04:30:26ZengElsevierEcotoxicology and Environmental Safety0147-65132023-01-01249114375Polystyrene nanoplastics promote the apoptosis in Caco-2 cells induced by okadaic acid more than microplasticsLinhong Yan0Zihua Yu1Peichun Lin2Shijie Qiu3Liuying He4Zijie Wu5Lihua Ma6Yanggao Gu7Lei He8Zhenqing Dai9Chunxia Zhou10Pengzhi Hong11Chengyong Li12College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang 524088, PR ChinaCollege of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang 524088, PR ChinaSchool of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, PR ChinaSchool of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, PR ChinaSchool of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, PR ChinaCollege of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang 524088, PR ChinaCollege of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang 524088, PR ChinaSchool of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, PR ChinaSchool of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, PR ChinaShenzhen Institute of Guangdong Ocean University, Shenzhen 518108, PR China; Corresponding author.College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang 524088, PR ChinaCollege of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang 524088, PR ChinaSchool of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, PR China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518108, PR China; Corresponding author at: School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, PR China.Microplastics (MPs) are widespread in the environment and can be ingested through food, water, and air, posing a threat to human health. In addition, MPs can have a potential combined effect with other toxic compounds. Polystyrene (PS) has been shown to enhance the cytotoxicity of okadaic acid (OA). However, it remains unclear whether this enhancement effect is related to the size of PS particles. In this study, we investigated the mechanism of the combined effect of PS microplastics (PS-MPs) or PS nanoplastics (PS-NPs) and OA on Caco-2 cells. The results indicated that PS-NPs enhanced the cytotoxicity of OA and induced endoplasmic reticulum (ER) stress-mediated apoptosis in Caco-2 cells, compared to PS-MPs. Specifically, PS-NPs and OA cause more severe oxidative stress, lactate dehydrogenase (LDH) release, and mitochondrial membrane depolarization. Furthermore, it induced intracellular calcium overload through store-operated channels (SOCs) and activated the PERK/ATF-4/CHOP pathway to cause ER stress. ER stress promoted mitochondrial damage and finally activated the caspase family to induce apoptosis. This study provided an indirect basis for the assessment of the combined toxicity of MPs or NPs with OA.http://www.sciencedirect.com/science/article/pii/S0147651322012155NanoplasticsMicroplasticsOkadaic acidCaco-2 cellsEndoplasmic reticulum stressApoptosis |
| spellingShingle | Linhong Yan Zihua Yu Peichun Lin Shijie Qiu Liuying He Zijie Wu Lihua Ma Yanggao Gu Lei He Zhenqing Dai Chunxia Zhou Pengzhi Hong Chengyong Li Polystyrene nanoplastics promote the apoptosis in Caco-2 cells induced by okadaic acid more than microplastics Ecotoxicology and Environmental Safety Nanoplastics Microplastics Okadaic acid Caco-2 cells Endoplasmic reticulum stress Apoptosis |
| title | Polystyrene nanoplastics promote the apoptosis in Caco-2 cells induced by okadaic acid more than microplastics |
| title_full | Polystyrene nanoplastics promote the apoptosis in Caco-2 cells induced by okadaic acid more than microplastics |
| title_fullStr | Polystyrene nanoplastics promote the apoptosis in Caco-2 cells induced by okadaic acid more than microplastics |
| title_full_unstemmed | Polystyrene nanoplastics promote the apoptosis in Caco-2 cells induced by okadaic acid more than microplastics |
| title_short | Polystyrene nanoplastics promote the apoptosis in Caco-2 cells induced by okadaic acid more than microplastics |
| title_sort | polystyrene nanoplastics promote the apoptosis in caco 2 cells induced by okadaic acid more than microplastics |
| topic | Nanoplastics Microplastics Okadaic acid Caco-2 cells Endoplasmic reticulum stress Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322012155 |
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