Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica
We entrapped lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica, and then applied entrapped lipase to the synthesis of biodiesel with soybean oil or waste cooking oil as a feedstock. The effects of reaction temperature, substrate molar ratio (methanol/oil) and n-hexane cont...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-04-01
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| Series: | Energies |
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| Online Access: | http://www.mdpi.com/1996-1073/6/4/2052 |
| _version_ | 1828138853652758528 |
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| author | Chi-Yang Yu Wei-Ting Lee Bing-Hong Tsai Shiow-Ling Lee Chia-Chi Lee I-Ching Kuan |
| author_facet | Chi-Yang Yu Wei-Ting Lee Bing-Hong Tsai Shiow-Ling Lee Chia-Chi Lee I-Ching Kuan |
| author_sort | Chi-Yang Yu |
| collection | DOAJ |
| description | We entrapped lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica, and then applied entrapped lipase to the synthesis of biodiesel with soybean oil or waste cooking oil as a feedstock. The effects of reaction temperature, substrate molar ratio (methanol/oil) and n-hexane content (w/w of oil) were evaluated using response surface methodology (RSM) combined with Box-Behnken design. The optimal reaction conditions for soybean oil were 43.6 °C, substrate molar ratio of 4.3%, and 75% n-hexane. The predicted and experimental values of biodiesel conversion were 79% and 76%, respectively. The optimal reaction conditions for waste cooking oil were 43.3 °C, substrate molar ratio of 5%, and 38% n-hexane. The predicted and experimental values of conversion were 68% and 67%, respectively. The conversion efficiency remained the same even after 1-month storage of entrapped lipase at 4 °C or room temperature. |
| first_indexed | 2024-04-11T18:43:57Z |
| format | Article |
| id | doaj.art-31cb8de22c164e5085b84dcb68fc6d7c |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-04-11T18:43:57Z |
| publishDate | 2013-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-31cb8de22c164e5085b84dcb68fc6d7c2022-12-22T04:08:54ZengMDPI AGEnergies1996-10732013-04-01642052206410.3390/en6042052Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic SilicaChi-Yang YuWei-Ting LeeBing-Hong TsaiShiow-Ling LeeChia-Chi LeeI-Ching KuanWe entrapped lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica, and then applied entrapped lipase to the synthesis of biodiesel with soybean oil or waste cooking oil as a feedstock. The effects of reaction temperature, substrate molar ratio (methanol/oil) and n-hexane content (w/w of oil) were evaluated using response surface methodology (RSM) combined with Box-Behnken design. The optimal reaction conditions for soybean oil were 43.6 °C, substrate molar ratio of 4.3%, and 75% n-hexane. The predicted and experimental values of biodiesel conversion were 79% and 76%, respectively. The optimal reaction conditions for waste cooking oil were 43.3 °C, substrate molar ratio of 5%, and 38% n-hexane. The predicted and experimental values of conversion were 68% and 67%, respectively. The conversion efficiency remained the same even after 1-month storage of entrapped lipase at 4 °C or room temperature.http://www.mdpi.com/1996-1073/6/4/2052biodiesellipasebiomimetic silicaresponse surface methodology |
| spellingShingle | Chi-Yang Yu Wei-Ting Lee Bing-Hong Tsai Shiow-Ling Lee Chia-Chi Lee I-Ching Kuan Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica Energies biodiesel lipase biomimetic silica response surface methodology |
| title | Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica |
| title_full | Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica |
| title_fullStr | Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica |
| title_full_unstemmed | Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica |
| title_short | Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica |
| title_sort | optimizing the production of biodiesel using lipase entrapped in biomimetic silica |
| topic | biodiesel lipase biomimetic silica response surface methodology |
| url | http://www.mdpi.com/1996-1073/6/4/2052 |
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